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LHP1048 - pFB-RSP5

Plasmid

#32434

Depositor

Linda Hicke

Insert

RSP5 (RSP5 Budding Yeast)

Expression

Insect

Promoter

P-P10 and P-Pol

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pFB1.HMBP.PrS.JARID2

Plasmid

#125166

Purpose

Expresses human JARID2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Publication

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

JARID2 (JARID2 Human)

Tags

His-MBP

Expression

Insect

Promoter

Polyhedrin

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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His-ZZ-TEV-SNAPf-BICD2N(1-400)

Plasmid

#111857

Purpose

N-terminal SNAPf tag on BICD2N (1-400) for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.

Depositor

Andrew Carter

Publication

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

BICD2N(1-400)

Tags

His-tag, SNAPf-tag, TEV site, and ZZ

Expression

Insect

Mutation

Optimised for Sf9 expression

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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p416-TEF-membrane localization signal-tagBFP-wrmScarlet11-TEF terminator

Plasmid

#158585

Purpose

Expresses membrane localized tagBFP with wrmScarlet11 C-terminal fusion in S. cerevisiae

Depositor

Cynthia Kenyon

Publication

Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

Insert

membrane localization signal-tagBFP-wrmScarlet11

Tags

tagBFP

Expression

Yeast

Mutation

GGGS linker

Promoter

TEF

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Vim-SNAP-tag2

Plasmid

#226518

Purpose

Mamalian cell expression of intermediate filaments (vimentin) fused to SNAP-tag2

Depositor

Kai Johnsson

Publication

Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

Insert

Vimentin-SNAP-tag2

Expression

Mammalian

Promoter

CMV

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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ClCry4 Y319D

Plasmid

#160649

Purpose

Expression of protein for various experiments including finding interaction partners

Depositor

Joseph Takahashi

Publication

Zoltowski et al Proc Natl Acad Sci U S A. 2019 Sep 24;11

Insert

Clcry4 PHR Y319D

Tags

Hexa his

Expression

Bacterial

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Cox8a(4x)-SNAP-tag2-T2A-EGFP

Plasmid

#226515

Purpose

Mamalian cell expression of SNAP-tag2 in the mitochondrial matrix and cytosolic EGFP

Depositor

Kai Johnsson

Publication

Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

Insert

Cox8a(4x)-SNAP-tag2-T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pFBD-SpyTag-noro-VLP

Plasmid

#165989

Purpose

Expresses norovirus-like particles with SpyTags presented on their surface for convenient decoration of SpyCatcher fusion proteins

Depositor

Vesa Hytönen

Publication

Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

Insert

SpyTag-noro-VLP (ORF2 Synthetic, norovirus)

Tags

SpyTag

Expression

Insect

Promoter

Polyhedrin

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFB1.HMBP.PrS.EPOP

Plasmid

#125170

Purpose

Expresses human EPOP in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Publication

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

EPOP (EPOP Human)

Tags

His-MBP

Expression

Insect

Promoter

Polyhedrin

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pFB-Flag-MyoD

Plasmid

#25994

Depositor

Jeffrey Dilworth

Publication

Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

Insert

MyoD (Myod1 Mouse)

Use

Baculovirus transfer vector

Tags

Flag

Available since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
pFB-GTE-COI1

Plasmid

#29516

Depositor

Ning Zheng

Publication

Sheard et al Nature. 2010 Nov 18. 468(7322):400-5.

Insert

COI1 (COI1 Mustard Weed)

Tags

GST and TEV cleavage site

Expression

Insect

Mutation

Note that the first AA in TEV site is R, not E

Available since

May 26, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pFBD-Akt13C(S473D)

Plasmid

#86577

Purpose

Expresses cleavable human full-length Akt1(S473D) phosphomimetic

Depositor

Thomas Leonard, Ivan Yudushkin

Publication

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Tags

10xHis

Expression

Insect

Mutation

3C cleavage site inserted at pos. 130; S473D phos…

Available since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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HDR-HaloPOLQ

Plasmid

#211637

Purpose

halo donor

Depositor

Agnel Sfeir

Publication

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

Puro-LoxHaloTag

Tags

flag

Expression

Mammalian

Promoter

SV40

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pFL-EGFP-3xFlag-DEST

Plasmid

#134903

Purpose

Baculovirus co-expression of GFP and gateway cloned protein with 3x-N-terminal Flag tag

Depositor

Andrew Deans

Publication

Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

Type

Empty backbone

Tags

3x-Flag

Expression

Insect

Promoter

polyhedron

Available since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pBC2FG

Plasmid

#31281

Depositor

Aziz Sancar

Publication

Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

Insert

Cry2 (Cry2 Mouse)

Tags

Flag and His

Expression

Insect

Available since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only
p1177-X1-8

Plasmid

#38150

Depositor

David Waugh

Publication

Austin et al Protein Expr Purif. 2011 May;77(1):53-61

Insert

Carboxypeptidase A

Tags

HHHHHHRR

Expression

Insect

Promoter

polyhedron

Available since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pET41a iGluSnFR

Plasmid

#119828

Purpose

Production of recombinant iGluSnFR, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Publication

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR

Tags

GST

Expression

Bacterial

Promoter

T7

Available since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pFB-GluCl_cryst

Plasmid

#31488

Depositor

Eric Gouaux

Publication

Hibbs et al Nature. 2011 Jun 2. 474(7349):54-60.

Insert

C. elegans glutamate-gated chloride channel alpha, crystallized construct

Tags

8x-His

Expression

Insect

Available since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
PGRP-SA

Plasmid

#21055

Depositor

Johann Deisenhofer

Publication

Chang et al PLoS Biol. 2004 Sep . 2(9):E277.

Insert

PGRP-SA (PGRP-SA Fly)

Tags

His

Expression

Insect

Available since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
Halo-53BP1 HRD

Plasmid

#207087

Purpose

Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous 53BP1 locus.

Depositor

Jens Schmidt

Publication

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

HaloTag with internal PuroR cassette flanked by human 53BP1 locus sequences

Tags

HaloTag

Expression

Mammalian

Promoter

Endogenous

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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