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We narrowed to 5,895 results for: SPI

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Showing: 1481 - 1500 of 5895 results

  2. pCAGEN-ActinVhh-sfGFP-P2A-HA-tdiRFP-caax (JDW 1532)

    Plasmid
    #242606
    Purpose
    A CAGGS driven expression vector containing an actin nanobody fused to sfGFP to label actin followed by a HA tagged tdiRFP fused to a caax tag to label the cell membrane.
    Depositor
    Joshua Wythe
    Article
    Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043
    Insert
    Actin Vhh-sfGFP-P2A-HA-tdiRFP-caax
    Expression
    Mammalian
    Promoter
    CAGGS
    Available Since
    Dec. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242606-plasmid-map-sequence-id-495400

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  9. pTol2-Crestin-zFUCCI-IRES-EGFP-caax (JDW 1516)

    Plasmid
    #242601
    Purpose
    A Tol2 expression vector containing the Crestin enhancer driving FUCCI to label cell cycle in neural crest cells followed by an IRES-EGFP-caax to label all neural crest cells green.
    Depositor
    Joshua Wythe
    Article
    Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043
    Insert
    mCerulean-zGEM-P2A-mCherry-zCdt1
    Use
    Zebrafish expression
    Promoter
    Crestin enhancer
    Available Since
    April 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    242601-plasmid-map-sequence-id-505637

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  10. pME-FastFucci-P2A-H2A-tdiRFP (JDW 1399)

    Plasmid
    #242557
    Purpose
    Gateway middle entry clone containing mammalian FastFUCCI reporter followed by H2A tdiRFP reporter; For visualizing cell cycle state. tdiRFP reporter labels all cells regardless of cell cycle state
    Depositor
    Joshua Wythe
    Article
    Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043
    Insert
    mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)
    Use
    Gateway Cloning
    Available Since
    Jan. 5, 2026
    Availability
    Academic Institutions and Nonprofits only
    242557-plasmid-map-sequence-id-495449

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  17. p5E-Crestin-pro (JDW 1320)

    Plasmid
    #229830
    Purpose
    A gateway compatible 5' entry clone containing the crestin gene followed by the mus musculus c Fos minimal promoter and then a rabbit beta globin intron to drive neural crest-specific gene expression
    Depositor
    Joshua Wythe
    Article
    Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043
    Insert
    Crestin promoter, c-fos minimal promoter, and b-globin intron
    Use
    Gateway Cloning
    Available Since
    Feb. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    229830-plasmid-map-sequence-id-458191

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  18. AP2u2-CMV-S_protein

    Plasmid
    #226456
    Purpose
    Mammalian expression of S protein of SARS-CoV2 under CMV promoter, tagged with 6His and released extracellular environment after expression for protein purification
    Depositor
    Urartu Seker
    Article
    Ozcelik et al ACS Pharmacol Transl Sci. 2024 Mar 21;7(
    Insert
    Spike Protein
    Tags
    6xHis Tag, Foldon Domain, and Thrombin Cleavage S…
    Expression
    Mammalian
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    226456-plasmid-map-sequence-id-458388

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  20. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

    Plasmid
    #182653
    Purpose
    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    mutant eukaryotic release factor 1
    Tags
    HA tag and nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    E55D and Y306A/Y384F (AF) double mutant of Methan…
    Promoter
    CMV and U6
    Available Since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    182653-plasmid-map-sequence-id-363026

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Showing: 1481 - 1500 of 5895 results