We narrowed to 5,304 results for: pcDNA3.1(+)

1. pcDNA3.1(+)/UUG-nLuc-3XFLAG

   Plasmid

   #127302

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from UUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1-Y1214F-VEGFR2-mCherry

   Plasmid

   #139609

   Purpose

   Expresses a mutant (Y1214F) VEGFR2-mCherry fusion protein in mammalian cells

   Depositor

   Michael Welsh

   Article

   Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

   Insert

   KDR (KDR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Blue opsin mcherry ---- pcDNA3.1

   Plasmid

   #62932

   Purpose

   Blue opsin tagged with mcherry on the C terminus

   Depositor

   Narasimhan Gautam

   Article

   Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

   Insert

   blue opsin
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1+ GEMIN6 myc HIS

   Plasmid

   #71659

   Purpose

   Mammalian expression of GEMIN6

   Depositor

   Gideon Dreyfuss

   Insert

   GEMIN6 (GEMIN6 Human)
   

   Use

   Tags

   HIS and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pCDNA3.1 hSCN5AdeltaSIV GFP Nter

   Plasmid

   #145770

   Purpose

   expresses human Nav1.5 lacking the SIV motif and tagged with GFP at its N-terminus in mammalian cells

   Depositor

   Hugues Abriel

   Article

   Petitprez et al Circ Res. 2011 Feb 4;108(3):294-304. doi

   Insert

   sodium voltage-gated channel alpha subunit 5 (SCN5A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Stop codon at Serine 2013

   Promoter

   CMV

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-V5H6-mPCSK3 D153A

   Plasmid

   #122681

   Purpose

   Expresses mutated mouse PCSK3 in mammalian cells (aspartic acid at position 153 mutated to alanine via site directed mutagenesis)

   Depositor

   Liming Pei

   Article

   Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

   Insert

   Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Aspartic Acid 153 to Alanine

   Promoter

   Available Since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LARG mtq sspB in pcDNA3.1

   Plasmid

   #90460

   Purpose

   RhoA mediated cell migration

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

   Inserts

   LARG DH-PH (aa 766–1138) (ARHGEF12 Human)
   mTq
   sspB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R73Q and deletion a.a. (234-239)

   Promoter

   Available Since

   June 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1-Tras light chain

   Plasmid

   #216294

   Purpose

   Expresses anti-HER2 Tras Light chain in mammalian cells. To be paired with Tras heavy chain to form the anti-HER2 Tras Fab

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   Tras light chain
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available Since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-Pert Light chain

   Plasmid

   #216310

   Purpose

   Expresses anti-HER2 Pert Light chain in mammalian cells. To be paired with Pert heavy chain to form the anti-HER2 Pert Fab

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   Pert Light chain
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available Since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1 human cofilin S3A

    Plasmid

    #50854

    Purpose

    expresses constituvely active cofilin in mammalian cells

    Depositor

    James Bamburg

    Article

    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

    Insert

    cofilin 1 S3A (CFL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    constitutively active, S3A

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pCDNA3.1(+)-myc-SNAP-GP135

    Plasmid

    #50376

    Purpose

    Express myc and SNAP-tagged GP135 in mammmalian cells

    Depositor

    Reika Watanabe

    Article

    Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

    Insert

    SNAP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.1(+)_Strep-Lrrk2-Myc

    Plasmid

    #161582

    Purpose

    expression of full-length mouse Lrrk2 with C-terminal Myc tag

    Depositor

    Maik Hintze

    Insert

    Lrrk2 (Lrrk2 Mouse)
    

    Use

    Tags

    Myc tag and StrepII

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1 6A CARD8 CARD-FLAG WT

    Plasmid

    #164020

    Purpose

    Mammalian expression vector encoding human CARD8 CARD with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-Ost4-GFP-MTn

    Plasmid

    #154351

    Purpose

    Mammalian expression of MTn

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    MTn
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-CMV-mCherry-hPMCA2x/b

    Plasmid

    #111571

    Purpose

    Expresses a plasma membrane calcium pump hPMCA2x/b

    Depositor

    Baljit Khakh

    Article

    Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

    Insert

    Plasma membrane calcium pump 2, variant x/b (ATP2B2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Sa-dCas9-NLS-3xFLAG/pcDNA3.1

    Plasmid

    #98041

    Purpose

    Expresses Sa-dCas9-NLS-3xFLAG in mammalian cells for enChIP analysis to purify specific genomic regions of interest

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

    Insert

    Sa-dCas9-NLS-3xFLAG
    

    Use

    CRISPR

    Tags

    3xFLAG tag and NLS (nuclear localization signal)

    Expression

    Mammalian

    Mutation

    D10A + H557A

    Promoter

    CMV

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FLAG-OTUB1 T134R-pcDNA3.1

    Plasmid

    #118211

    Purpose

    Expresses FLAG-OTUB1 T134R mutant in mammalian cells

    Depositor

    Cynthia Wolberger

    Article

    Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

    Insert

    OTUB1 (OTUB1 Human)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    changed threonine 134 to arginine

    Promoter

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Rab8-T22N(Flag) in pcDNA3.1neo

    Plasmid

    #46784

    Purpose

    expression of T22N Rab8a in mammalian cells

    Depositor

    Terry Hébert

    Article

    Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

    Insert

    RAB8A T22N (RAB8A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T22N dominant negative

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.1-Mito-AcGFP-MTn

    Plasmid

    #154350

    Purpose

    Mammalian expression of MTn

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    MTn
    

    Use

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1(+)-ExRai-AKAR-NES

    Plasmid

    #118407

    Purpose

    Cytosol-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-AKAR-NES
    

    Use

    Tags

    Nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge