We narrowed to 5,328 results for: pcdna3.1

1. pcDNA3.1-hRIPK1-Flag-delDD

   Plasmid

   #112494

   Purpose

   Mammalian expression of the indicated Flag- hRIPK1 mutant. Please note Flag tag is N-terminal.

   Depositor

   Junying Yuan

   Insert

   RIPK1 (RIPK1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deletion of DD domain (AA584-671 deleted)

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)/GUG-nLuc-3XFLAG

   Plasmid

   #127301

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-2xFLAG-2xSTREP-CCND1

   Plasmid

   #172648

   Purpose

   Expresses 2xFLAG-2xSTREP-tagged cyclin D1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1/TO-myc-BirA*-NES

   Plasmid

   #112714

   Purpose

   For tetracycline-inducible expression of myc-BirA*-NES in mammalian cells. For BioID studies.

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

   Insert

   HIV Rev nuclear export signal (NES)
   

   Expression

   Mammalian

   Available Since

   June 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-N-Myc_DED/AAA USP7

   Plasmid

   #131253

   Purpose

   Mammalian expression of an N-terminally Myc-tagged USP7 mutant, with mutations in UBL2 (DE758AA_D764A)

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Contains point mutations that convert USP7 aspart…

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 CMV NAPA-A BGH

   Plasmid

   #92277

   Purpose

   FRET probe; extracellular membrane tethered eGFP

   Depositor

   Baljit Khakh

   Article

   Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

   Insert

   Neuron-astrocyte proximity assay - Astrocyte
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMJA219= pcDNA3.1V5-completeTRABV-CMVp

   Plasmid

   #116878

   Purpose

   mammalian expression vector containing TCR sequences

   Depositor

   Marcos Alcocer

   Article

   Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

   Insert

   complete TRAV10-TRBV25-CMVp rabbitpolyA (TRAV10, TRBV25-1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV Promoter

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-Y1214F-VEGFR2-mCherry

   Plasmid

   #139609

   Purpose

   Expresses a mutant (Y1214F) VEGFR2-mCherry fusion protein in mammalian cells

   Depositor

   Michael Welsh

   Article

   Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

   Insert

   KDR (KDR Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Blue opsin mcherry ---- pcDNA3.1

   Plasmid

   #62932

   Purpose

   Blue opsin tagged with mcherry on the C terminus

   Depositor

   Narasimhan Gautam

   Article

   Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

   Insert

   blue opsin
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+)/UUG-nLuc-3XFLAG

    Plasmid

    #127302

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from UUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1+ GEMIN6 myc HIS

    Plasmid

    #71659

    Purpose

    Mammalian expression of GEMIN6

    Depositor

    Gideon Dreyfuss

    Insert

    GEMIN6 (GEMIN6 Human)
    

    Tags

    HIS and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pCDNA3.1 hSCN5AdeltaSIV GFP Nter

    Plasmid

    #145770

    Purpose

    expresses human Nav1.5 lacking the SIV motif and tagged with GFP at its N-terminus in mammalian cells

    Depositor

    Hugues Abriel

    Article

    Petitprez et al Circ Res. 2011 Feb 4;108(3):294-304. doi

    Insert

    sodium voltage-gated channel alpha subunit 5 (SCN5A Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Stop codon at Serine 2013

    Promoter

    CMV

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Human NCKX4-AS-long (pcDNA3.1+)

    Plasmid

    #75211

    Purpose

    Mammalian expression of SLC24A4

    Depositor

    Jonathan Lytton

    Article

    Li et al J Biol Chem. 2002 Dec 13;277(50):48410-7

    Insert

    SLC24A4 (SLC24A4 Human)
    

    Expression

    Mammalian

    Mutation

    alternatively spliced exon present

    Promoter

    CMV

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1 human cofilin S3A

    Plasmid

    #50854

    Purpose

    expresses constituvely active cofilin in mammalian cells

    Depositor

    James Bamburg

    Article

    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

    Insert

    cofilin 1 S3A (CFL1 Human)
    

    Expression

    Mammalian

    Mutation

    constitutively active, S3A

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pCDNA3.1(+)-myc-SNAP-GP135

    Plasmid

    #50376

    Purpose

    Express myc and SNAP-tagged GP135 in mammmalian cells

    Depositor

    Reika Watanabe

    Article

    Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

    Insert

    SNAP
    

    Expression

    Mammalian

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1(+)_Strep-Lrrk2-Myc

    Plasmid

    #161582

    Purpose

    expression of full-length mouse Lrrk2 with C-terminal Myc tag

    Depositor

    Maik Hintze

    Insert

    Lrrk2 (Lrrk2 Mouse)
    

    Tags

    Myc tag and StrepII

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. LARG mtq sspB in pcDNA3.1

    Plasmid

    #90460

    Purpose

    RhoA mediated cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

    Inserts

    LARG DH-PH (aa 766–1138) (ARHGEF12 Human)
    mTq
    sspB
    

    Expression

    Mammalian

    Mutation

    R73Q and deletion a.a. (234-239)

    Available Since

    June 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-V5H6-mPCSK3 D153A

    Plasmid

    #122681

    Purpose

    Expresses mutated mouse PCSK3 in mammalian cells (aspartic acid at position 153 mutated to alanine via site directed mutagenesis)

    Depositor

    Liming Pei

    Article

    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

    Insert

    Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
    

    Expression

    Mammalian

    Mutation

    changed Aspartic Acid 153 to Alanine

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-Ost4-GFP-MTn

    Plasmid

    #154351

    Purpose

    Mammalian expression of MTn

    Depositor

    Wanzhong He

    Article

    Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

    Insert

    MTn
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Sa-dCas9-NLS-3xFLAG/pcDNA3.1

    Plasmid

    #98041

    Purpose

    Expresses Sa-dCas9-NLS-3xFLAG in mammalian cells for enChIP analysis to purify specific genomic regions of interest

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

    Insert

    Sa-dCas9-NLS-3xFLAG
    

    Use

    CRISPR

    Tags

    3xFLAG tag and NLS (nuclear localization signal)

    Expression

    Mammalian

    Mutation

    D10A + H557A

    Promoter

    CMV

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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