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pLX-TRV2

Plasmid

#180516

Purpose

Agrobacterium tumefaciens-based expression of TRV2 including a PEBV heterologous promoter and a polylinker

Depositor

Jose-Antonio Daros

Article

Aragones et al Biotechnol J. 2022 Mar 24:e2100504. doi:

Insert

Tobacco rattle virus RNA2 with a PEBV heterologous promoter and a polylinker

Expression

Plant

Promoter

35S CaMV

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_HIF1A_WT

Plasmid

#82129

Purpose

Gateway Donor vector containing HIF1A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HIF1A (HIF1A Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-Exo70

Plasmid

#53761

Purpose

pEGFP-C3 plasmid that expresses Exo70

Depositor

Channing Der

Article

Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

Insert

Exo70 (EXOC7 Human)

Tags

GFP

Expression

Mammalian

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-J chain

Plasmid

#145146

Purpose

Mammalian expression plasmid for J chain of polymeric antibody classes

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

immunoglobulin J chain (JCHAIN Human)

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pBS185 CMV-Cre

Plasmid

#11916

Purpose

Mammalian expression of Cre recombinase driven by the human cytomegalovirus IE promoter

Depositor

Brian Sauer

Article

Sauer et al New Biol. 1990 May . 2(5):441-9.

Insert

cre (cre bacteriophage P1)

Use

Cre/Lox

Expression

Mammalian

Promoter

CMV IE

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pCRISPR-RFP entry

Plasmid

#192278

Purpose

Entry vector for cloning MmCas12m spacers. RFP flanked by MmCas12m CRISPR repeats can be digested out with BbsI.

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

RFP flanked by type V-M CRISPR repeat sequences

Use

CRISPR

Expression

Bacterial

Promoter

PJ23119

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

Plasmid

#118154

Purpose

Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Jorge Ferrer

Insert

KRAB-dCas9-P2A-BlastR

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Mutation

See depositor comments below

Promoter

EF1a core and U6

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1P CISD2_2

Plasmid

#160776

Purpose

Suppress CISD2

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shCISD2_2

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P CISD2_1

Plasmid

#160775

Purpose

Suppress CISD2

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shCISD2_1

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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plenti-CAG-gate-FLAG-IRES-GFP

Plasmid

#107398

Purpose

Lentiviral gateway destination vector

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Use

Lentiviral; Gateway destination

Tags

FLAG

Expression

Mammalian

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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