We narrowed to 5,032 results for: plasmid dna

1. LT1_75_Donor_Tag_RPL13A-mTurq2

   Plasmid

   #194415

   Purpose

   Plasmid for genome integration of mTurquoise2 CDS to tag native RPL13A for biosensors RibPro and UPRpro

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

   Insert

   mTurquoise2 CDS
   

   Use

   Synthetic Biology; Used as donor dna in genome in…

   Tags

   Expression

   Yeast

   Mutation

   NA

   Promoter

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHC_DYOLacI-R

   Plasmid

   #102922

   Purpose

   pUC-19 derivative reporter plasmid for the screening of Transcription Factors (TFs) carrying the DNA-binding domain of LacI

   Depositor

   George Church

   Article

   Juarez et al Nat Commun. 2018 Aug 6;9(1):3101. doi: 1

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSP569

   Plasmid

   #139499

   Purpose

   Repair DNA plasmid to insert PP7 stem loops in the endogenous STL1 locus after double strand break in S. cerevisiae

   Depositor

   Serge Pelet

   Article

   Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

   Insert

   pSTL1 24xPP7sl STL1 100 - 600
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   WT

   Promoter

   pSTL1

   Available since

   June 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBluescript alpha-SSeCKS

   Plasmid

   #61295

   Purpose

   contains rat alpha SSeCKS cDNA

   Depositor

   Irwin Gelman

   Insert

   alpha SSeCKS (Akap12 Rat)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   cDNA with 1kb of 3' UTR and 150bp of 5'…

   Promoter

   T7

   Available since

   Dec. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

5. BirA(R118G)-HA destination vector

   Plasmid

   #53581

   Purpose

   To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID)

   Depositor

   Karl Kramer

   Type

   Empty backbone

   Use

   Tags

   BirA(R118G) and HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. POLQA

   Plasmid

   #74645

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   POLQA (POLQ Human)
   

   Use

   Baculovirus

   Tags

   His-6-TEV

   Expression

   Mutation

   Promoter

   PH

   Available since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. HA-Clover-HIF-1alpha Wildtype

   Plasmid

   #163365

   Purpose

   Express HA- and Clover-tagged HIF-1alpha wildtype plasmid

   Depositor

   Violaine Sée

   Insert

   Hypoxia Inducible Factor 1 alpha (HIF1A Human)
   

   Use

   Tags

   HA- and Clover-tagged

   Expression

   Mammalian

   Mutation

   Wildtype

   Promoter

   CMV Promoter

   Available since

   Jan. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SIN40C.SFFV.MCS.IRES.GFP

   Plasmid

   #169280

   Purpose

   Constitutive overexpression of cDNA with GFP as a reporter fluorescent protein

   Depositor

   Dirk Heckl

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SIN40C.SFFV.MCS.IRES.dTomato

   Plasmid

   #169279

   Purpose

   Constitutive overexpression of cDNA with dTomato as a reporter fluorescent protein

   Depositor

   Dirk Heckl

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pOME240P

    Plasmid

    #182602

    Purpose

    T15

    Depositor

    Dirk Dittmer

    Insert

    T15
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMflT-o2

    Plasmid

    #101310

    Purpose

    Contains: oriC2 fragment of M. florum L1 replication origin (dnaA/dnaN intergenic region), colE1 replication origin, recoded tetM resistance gene.

    Depositor

    Sébastien Rodrigue

    Article

    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

    Insert

    oriC2 of M. florum strain L1 (dnaA/dnaN intergenic region)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mBeta4/pCNA3.1

    Plasmid

    #197316

    Purpose

    Plasmid expressing an antigen used to generate an antibody that targets the BK beta4 potassium channel subunit

    Depositor

    Robert Brenner

    Insert

    Slo B4 (Kcnmb4 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pgLAP1

    Plasmid

    #19702

    Depositor

    Peter Jackson

    Type

    Empty backbone

    Use

    Tags

    EGFP, S peptide, and TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pgLAP2

    Plasmid

    #19703

    Depositor

    Peter Jackson

    Type

    Empty backbone

    Use

    Tags

    Flag, S peptide, and TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. SIN40C.TRE.MCS.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

    Plasmid

    #169283

    Purpose

    Doxycycline-inducible overexpression of cDNA with dTomato as a reporter fluorescent protein

    Depositor

    Dirk Heckl

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. cTRE-LSL-GFP-IRES-Cas9-sgCR8

    Plasmid

    #135668

    Purpose

    Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus control-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange

    Depositor

    Florian Karreth

    Insert

    sgCR8
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. cTRE-LSL-GFP-IRES-Cas9-sgPtenX1.1

    Plasmid

    #135669

    Purpose

    Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus Pten-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange

    Depositor

    Florian Karreth

    Insert

    sgPten
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCEFL

    Plasmid

    #38121

    Depositor

    JS Gutkind

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1

    Available since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pInt-ERCreER

    Plasmid

    #190040

    Purpose

    This is an integration donor plasmid to insert a DNA fragment for doxycycline inducible ERT2-Cre-ERT2 at the AAVS1 locus. It works with an AAVS1 targeting CRISPR/Cas9 construct (Addgene #72833).

    Depositor

    Kayoko Tanaka

    Article

    Hindul et al Biol Open. 2022 Feb 15;11(2). pii: 27408

    Insert

    ERT2-Cre-ERT2 flanked by AAVS1
    

    Use

    CRISPR and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRN3P_T3_ABE8e_IVT

    Plasmid

    #201676

    Purpose

    Plasmid to be used as DNA template for in vitro RNA transcription of the ABE8e base editor (A to G) by T3 RNA polymerase. The plasmid contains optimised 5'UTR and 3'UTR to improve protein expression.

    Depositor

    Timo Otonkoski

    Article

    Jalil et al Am J Hum Genet. 2024 Apr 4;111(4):714-72

    Insert

    ecTadA(8e)-nSpCas9
    

    Use

    CRISPR; Vector for in-vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T3 promoter

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. KO101: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-Dnmt3a3L

    Plasmid

    #121833

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    x-dCas9(3.7)/Dnmt3a-3L (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCRISPR-Cas9/CD4-TK2

    Plasmid

    #104397

    Purpose

    The designed sgRNA cloned into this plasmid directs the specific DNA cleavage exerted by Cas9 nuclease in a region of exon 5 of the human TK2 gene. The plasmid also includes the Cas9 nuclease and CD4.

    Depositor

    Jeronimo Blanco

    Article

    Meca-Cortes et al Mol Ther Nucleic Acids. 2017 Sep 15;8:39

    Insert

    TK2 (TK2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. IP803: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-Dnmt3a3L

    Plasmid

    #121826

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Sa-dCas9/Dnmt3a-3L (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PVALB mAb_BCN14.1.1A2_HC_Rat IgG2a

    Plasmid

    #182894

    Purpose

    Mammalian expression of PVALB rat mAb HC gene

    Depositor

    Heng Zhu

    Insert

    anti-PVALB (human) heavy chain (PVALB Rat)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    No

    Promoter

    CMV

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Slack Slo2.2 max1-ECFP

    Plasmid

    #197318

    Purpose

    Plasmid expressing an antigen used to generate an antibody that targets the Slack K+ channel subunit

    Depositor

    Christopher Lingle

    Insert

    KCNT1/Slo2.2/Slack K+ channel (Kcnt1 Mouse)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Nemato

    Plasmid

    #24006

    Depositor

    Lawrence Cohen

    Insert

    VSFP
    

    Use

    Tags

    Cerulean and Citruline

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pOME0294M

    Plasmid

    #182623

    Purpose

    KSHV anti-RTA_3Kb_Reverse

    Depositor

    Dirk Dittmer

    Insert

    KSHV anti-RTA_3Kb_Reverse
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion at 2853

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. GFP-noPS

    Plasmid

    #177943

    Purpose

    Transient mammalian expression of eGFP. Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Luc-noPS

    Plasmid

    #177940

    Purpose

    Transient mammalian expression of Luc2 (firefly luciferase). Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    Luc2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. L-SACSIN-74

    Plasmid

    #166526

    Purpose

    scFv of a human scaffold targeting Sacsin, DnaJ homolog subfamily C member 29. Antigen coverage aa 84-339 of 4579

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, Sortag, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pOME0295M

    Plasmid

    #182624

    Purpose

    KSHV Reverse Sequence Negative control Kaposinclouster_RevCompl-KapATGdel_Syn

    Depositor

    Dirk Dittmer

    Insert

    KSHV Kaposincluster_RevCompl_KapATGdel_SYN_rev
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PVALB mAb_BCN14.1.1A2_LC_Rat Ig Kappa

    Plasmid

    #182895

    Purpose

    Mammalian expression of PVALB rat mAb LC gene

    Depositor

    Heng Zhu

    Insert

    anti-PVALB (human) light chain (PVALB Rat)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    No

    Promoter

    CMV

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pdCas9-DNMT3A-PuroR (ANV)_v2

    Plasmid

    #74408

    Purpose

    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A Synthetic, S. pyogenes, Human)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E756A inactiva…

    Promoter

    CBh

    Available since

    April 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pTBL1269 pLenti-CHYRON17-pEF1a(short)-Luc2-P2A-tdTomato-T2A-TdT

    Plasmid

    #163643

    Purpose

    Makes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, tdTomato, and human TdT.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6-CHYRON17 hgRNA
    Luc2-P2A-tdTomato-T2A-human TdT (DNTT Human)
    

    Use

    Lentiviral

    Tags

    Luc2-P2A-tdTomato-T2A

    Expression

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    EFS and pU6

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. CDKN1A (p21) targeting gRNA

    Plasmid

    #215319

    Purpose

    Expresses gRNA targeting CDKN1A (p21) and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. mAzGreen-Cyclin D3-NeoR

    Plasmid

    #215061

    Purpose

    Expresses mAzGreen-Cyclin D3 in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCND3 (CCND3 Human)
    

    Use

    Tags

    mAzGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. mAzGreen-Cyclin D2-NeoR

    Plasmid

    #215062

    Purpose

    Expresses mAzGreen-Cyclin D2 in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCND2 (CCND2 Human)
    

    Use

    Tags

    mAzGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. mVenus-Cyclin D1-NeoR

    Plasmid

    #215068

    Purpose

    Expresses mVenus-Cyclin D1 in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. mAzGreen-Cyclin D1-NeoR

    Plasmid

    #215060

    Purpose

    Expresses mAzGreen-Cyclin D1 in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Tags

    mAzGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. cTRE-PtenWT

    Plasmid

    #135663

    Purpose

    Introduce Dox-inducible (TRE promoter) wildtype Pten cDNA into (ES) cells by recombination-mediated cassette exchange

    Depositor

    Florian Karreth

    Insert

    Pten (Pten Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge