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EC71100

Plasmid

#154068

Purpose

Level 0 Golden Gate vector, pHvHSP17

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

pHvHSP17

Use

Standard cloning vector used by geneart for synth…

Mutation

All BsaI, Esp3I and BpiI restriction sites were r…

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX4T-HEPN

Plasmid

#75296

Purpose

Expresses Sacsin HEPN domain (4441-4579) in fusion with GST (bacterial)

Depositor

Kalle Gehring

Article

Kozlov et al J Biol Chem. 2011 Jun 10;286(23):20407-1

Insert

Human Sacsin 4441-4579

Tags

GST-Tag

Expression

Bacterial

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pXTK066 [Con-Pro_nptII-Broad_PartType8]

Plasmid

#229303

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains Constitutive-Promoter_nptII-Broad_PartType8

Depositor

Michael Springer

Article

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Insert

nptII Promoter (Broad)

Expression

Bacterial

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Chronos-mT-Sapphire-NRN

Plasmid

#149692

Purpose

For packaging and expression of Chronos-mT-Sapphire featuring a neuritin (NRN) 3' UTR in neurons via AAV

Depositor

Robert Malenka

Article

Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

Insert

Chronos

Use

AAV

Tags

mT-Sapphire

Expression

Mammalian

Promoter

hSyn

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-PGAL1-GFP

Plasmid

#41614

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Available Since

Dec. 11, 2012

Availability

Academic Institutions and Nonprofits only

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pCI-MS2V5-PABPC1 MLLEmut

Plasmid

#65808

Purpose

expression of PABPC1-MS2 fusion. Mutation of C-terminal MLLE domain.

Depositor

Niels Gehring

Article

Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

Insert

PABPC1 (PABPC2 Human)

Tags

MS2 stem loops and V5

Expression

Mammalian

Mutation

MLLEmut

Promoter

CMV

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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EC71022

Plasmid

#159608

Purpose

Level 0.5 Golden Gate Vector, loxP-mCHERRY-HDEL-t35s-loxP

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

loxP-mCherry-HDEL-t35S-loxP

Use

Cre/Lox and Synthetic Biology

Mutation

All BsaI, Esp3I and BPiI restriction sites were r…

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P3nmt1-3HA

Plasmid

#39283

Depositor

Jurg Bahler, John Pringle

Article

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

nmt1 promoter (nmt1 Fission Yeast)

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Mutation

-1163 to +6 of nmt1 locus

Available Since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChrimsonR-LSSmOrange-NRN

Plasmid

#149693

Purpose

For packaging and expression of ChrimsonR-LSSmOrange featuring a neuritin (NRN) 3' UTR in neurons via AAV

Depositor

Robert Malenka

Article

Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

Insert

ChrimsonR

Use

AAV

Tags

LSSmOrange

Expression

Mammalian

Promoter

hSyn

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin 141-465

Plasmid

#45971

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin 141-465 (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Dele…

Promoter

tac

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only

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NG0886 pCI-TPI-WT-4H

Plasmid

#65801

Purpose

NMD reporter mRNA

Depositor

Niels Gehring

Article

Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

Insert

TPI (TPI1 Human)

Tags

4H (4 copies of short beta-globin 3'UTR for …

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)_mtZFN(-)2G

Plasmid

#62799

Purpose

mtZFN2G architecture bearing (-) FokI domain

Depositor

Michal Minczuk

Article

Gammage et al EMBO Mol Med. 2014 Apr;6(4):458-66. doi:

Insert

mtZFN(-)2G

Tags

Internal FLAG tag immediately after F1B mitochond…

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)_mtZFN(+)2G

Plasmid

#62798

Purpose

mtZFN2G architecture bearing (+) FokI domain

Depositor

Michal Minczuk

Article

Gammage et al EMBO Mol Med. 2014 Apr;6(4):458-66. doi:

Insert

mtZFN(+)2G

Tags

Internal HA tag immediately after F1B mitochondri…

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-PGAL1-GST

Plasmid

#41611

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pMV-HIS3

Plasmid

#65333

Purpose

standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker HIS3 which could be released and ligated to series of TUs

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

HIS3

Use

Synthetic Biology

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pPA016 (delta 86-130 Parkin)

Plasmid

#102357

Purpose

expresses Rat Parkin with residues 86-130 deleted

Depositor

Kalle Gehring

Article

Sauve et al EMBO J. 2015 Oct 14;34(20):2492-505. doi

Insert

Parkin (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

deleted residues 86 to 130

Promoter

tac

Available Since

Nov. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-His3MX6-PGAL1-3HA

Plasmid

#41610

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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plexm-mouse LRRC33

Plasmid

#111601

Purpose

Express mouse LRRC33 in 293T cells

Depositor

Timothy Springer

Article

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

mouse LRRC33 (Nrros Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Starts at aa 18

Available Since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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Sm-Myocardin Omni-pShuttle

Plasmid

#122122

Purpose

Expression of the smooth muscle version of myocardin

Depositor

Paul Herring

Article

Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

Insert

Myocardin (Myocd Mouse)

Tags

Omni

Expression

Mammalian

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-globin_del5UTR_WT-xrRNA-4H

Plasmid

#108375

Purpose

FRT integration of wild type beta-globin reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

Depositor

Niels Gehring

Article

Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

Insert

Wild type beta-globin reporter with MVE xrRNA and 4H probe binding sites

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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