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Plasmid#102357Purposeexpresses Rat Parkin with residues 86-130 deletedDepositorInsertParkin (Prkn Rat)
UseTagsGSTExpressionBacterialMutationdeleted residues 86 to 130PromotertacAvailable sinceNov. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-PGAL1-GFP
Plasmid#41614DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutationPromoterAvailable sinceDec. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCI-globin_del5UTR_WT-xrRNA-4H
Plasmid#108366PurposeExpresses wild type beta-globin reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertWild type beta-globin reporter with MVE xrRNA and 4H probe binding sites
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
NG0886 pCI-TPI-WT-4H
Plasmid#65801PurposeNMD reporter mRNADepositorInsertTPI (TPI1 Human)
UseTags4H (4 copies of short beta-globin 3'UTR for …ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)_mtZFN(-)2G
Plasmid#62799PurposemtZFN2G architecture bearing (-) FokI domainDepositorInsertmtZFN(-)2G
UseTagsInternal FLAG tag immediately after F1B mitochond…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)_mtZFN(+)2G
Plasmid#62798PurposemtZFN2G architecture bearing (+) FokI domainDepositorInsertmtZFN(+)2G
UseTagsInternal HA tag immediately after F1B mitochondri…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
plexm-mouse LRRC33
Plasmid#111601PurposeExpress mouse LRRC33 in 293T cellsDepositorInsertmouse LRRC33 (Nrros Mouse)
UseTagsFLAGExpressionMammalianMutationStarts at aa 18PromoterAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMV-LEU2
Plasmid#65334Purposestandard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker LEU2 which could be released and ligated to series of TUsDepositorInsertLEU2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-hSt3Cas9 (pXK-1721)
Plasmid#208828PurposeCRISPR/hSt3Cas9 expression vector for Animal gene editing, harboring the humanized St3Cas9 gene and the corresponding optimized SP1.gRNA. Key Construct: mU6- SP1.gRNA-CMV-hSt3Cas9.DepositorTypeEmpty backboneUseCRISPR; Stcas9TagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA5/FRT/TO-globin_del5UTR_WT-xrRNA-4H
Plasmid#108375PurposeFRT integration of wild type beta-globin reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertWild type beta-globin reporter with MVE xrRNA and 4H probe binding sites
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-His3MX6-PGAL1-3HA
Plasmid#41610DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTags3xHA, A. gossypii translation elongation factor 1…ExpressionMutationPromoterAvailable sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
Sm-Myocardin Omni-pShuttle
Plasmid#122122PurposeExpression of the smooth muscle version of myocardinDepositorInsertMyocardin (Myocd Mouse)
UseTagsOmniExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGWB21_TaNAM-A1
Plasmid#124572PurposeExpresses full-length TaNAM-A1 with an N-terminal 10x Myc tag under the 35s promoter.DepositorInsertTaNAM-A1
UseTags10xMycExpressionPlantMutationPromoter35sAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin 219-465
Plasmid#45972DepositorInsertParkin 219-465 (Prkn Rat)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Dele…PromotertacAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCI-TPI_WT-xrRNA-4H
Plasmid#108368PurposeExpresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertWild type TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_10A
Plasmid#91207PurposeDirect Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: noneDepositorInsert35S:AtCas9 + AtU6:gRNA
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCI-globin_del5UTR_PTC39-xrRNA-4H
Plasmid#108367PurposeExpresses beta-globin reporter with premature termination codon (PTC39); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertPTC39 beta-globin reporter with MVE xrRNA and 4H probe binding sites
UseTagsExpressionMammalianMutationPTC39PromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-globin_del5UTR_PTC39-xrRNA-4H
Plasmid#108376PurposeFRT integration of beta-globin reporter with premature termination codon (PTC39); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertPTC39 beta-globin reporter with MVE xrRNA and 4H probe binding sites
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin W403A-C431S
Plasmid#45977DepositorInsertParkin W403A-C431S (Prkn Rat)
UseTagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Doub…PromotertacAvailable sinceSept. 5, 2013AvailabilityAcademic Institutions and Nonprofits only