Addgene

We narrowed to 8,073 results for: gnal

Showing: 1501 - 1520 of 8073 results

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NbV5-LgBit

Plasmid

#201475

Purpose

NanoBit detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-LgBit

Use

Tags

LgBit

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pVITRO1-mCardinal-CAXX

Plasmid

#184451

Purpose

Plasma membrane-targeted mCardinal expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

mCardinal-CAAX

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pPUR-hU6-sgRNA-Sirius-8XMS2

Plasmid

#121942

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-8XMS2

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Mutation

Promoter

human U6 promoter

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-APEX2-GFP-3xFLAG-PuroR

Plasmid

#221238

Purpose

CRISPaint gene tagging

Depositor

Jonathan Kagan

Article

Fisch et al Nature. 2024 Jul;631(8021):635-644. doi:

Insert

APEX2-GFP-3xFLAG

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Peredox

Plasmid

#73807

Purpose

Expresses the Peredox fluorescent sensor protein (for NADH/NAD ratio) in mammalian cells (and can be packaged as AAV)

Depositor

Gary Yellen

Article

Mongeon et al Antioxid Redox Signal. 2016 Mar 18.

Insert

Peredox

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pCI HA NEDD4 DD

Plasmid

#26999

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

NEDD4 (NEDD4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

C867A

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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opto-RhoGEF-mCherry_pcDNA3.1

Plasmid

#164473

Purpose

Expresses opto-RhoGEF (BcLOV4-GGGSx2-ARHGEF11dh-GGGSx2-mCherry) in a pcDNA3.1 backbone.

Depositor

Brian Chow

Article

Berlew et al Adv Biol. 2021 Sep;5(9):e2100810. doi: 1

Insert

opto-RhoGEF

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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NbV5-eGFP

Plasmid

#201473

Purpose

Indirect microscopy/imaging of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-eGFP

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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MPZ(P0)promoter pGL3-TATA

Plasmid

#21259

Depositor

Jerry Crabtree

Article

Kao et al Science. 2009 Jan 30. 323(5914):651-4.

Insert

myelin protein zero promoter (Mpz Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2009

Availability

Academic Institutions and Nonprofits only

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V245 pCEP-4HA B56alpha

Plasmid

#14532

Depositor

David Virshup

Article

Seeling et al Science. 1999 Mar 26. 283(5410):2089-91.

Insert

PPP2R5A B56 alpha regulatory subunit of PP2A (PPP2R5A Human)

Use

Tags

4 HA

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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TagBFP2-PIP4K2B

Plasmid

#202728

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mTagBFP2:PIP4K2B (PIP4K2B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PKA-SPARK

Plasmid

#106920

Purpose

Express PKA-SPARK, an activity reporter of protein kinase A in mammalian cells.

Depositor

Xiaokun Shu

Article

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Insert

PKA-SPARK

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pEF.myc.ER-E2-Crimson

Plasmid

#38770

Purpose

In vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)

Depositor

Benjamin Glick

Article

Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.

Insert

E2-Crimson

Use

Tags

ER retention signal (KDEL), ER signal sequence (M…

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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TagBFP2- PIP4K2BD278K

Plasmid

#202729

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mTagBFP2:PIP4K2B (D278K) (PIP4K2B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ERK-SPARK

Plasmid

#106921

Purpose

Express ERK-SPARK, an activity reporter of ERK in mammalian cells.

Depositor

Xiaokun Shu

Article

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Insert

ERK-SPARK

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-supervillin

Plasmid

#13040

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2006

Availability

Academic Institutions and Nonprofits only

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human syndecan 2 wt

Plasmid

#64970

Purpose

Constitutive expression in mammalian cells

Depositor

Enric Espel

Article

Rovira-Clave et al Cell Signal. 2014 Jul;26(7):1489-99. doi

Insert

syndecan 2 (SDC2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 5, 2015

Availability

Academic Institutions and Nonprofits only
pRK7-FLAG-TSC1

Plasmid

#8995

Depositor

John Blenis

Article

Tee et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

Insert

TSC1 (TSC1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 11, 2005

Availability

Academic Institutions and Nonprofits only

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pelBHisTEV

Plasmid

#157741

Purpose

empty vector; TEV-protease-removable N-term PelB signal sequence +His10; potential C-term His6; T7lac promoter; Kan-R; avoids added residues in final protein since cloning uses BseRI restriction sites

Depositor

Debra Hansen

Type

Empty backbone

Use

Tags

PelB signal sequence + His10-tag + TEV protease c…

Expression

Bacterial

Mutation

Promoter

T7lac

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pC1-CMV-DAAO-NES

Plasmid

#141107

Purpose

Expresses DAAO with nuclear export signal in mammalian cells

Depositor

Vsevolod Belousov

Article

Bogdanova et al Curr Protoc Chem Biol. 2017 Jun 19;9(2):

Insert

DAAO-NES

Use

Tags

Nuclear export signal

Expression

Mammalian

Mutation

deleted DAAO amino acids 367-368

Promoter

CMV

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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