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pcDNA3_SARS-CoV-Spike[WIV1 RBD]

Plasmid

#190087

Purpose

express chimeric SARS-CoV spike with the WIV-1 receptor binding domain

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

SARS-CoV S (S Synthetic)

Use

Tags

Rhodopsin C9

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SARS-CoV-Spike[Khosta1 RBD]

Plasmid

#190082

Purpose

express chimeric SARS-CoV spike with the Khosta-1 receptor binding domain

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

SARS-CoV S (S Synthetic)

Use

Tags

Rhodopsin C9

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcdna3 T7 Jip1b(282-707)(C605S, C631S, C643S and C696T)

Plasmid

#52134

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b)(282-707)(M1-5) (Mapk8ip1 Mouse)

Use

Tags

T7

Expression

Mammalian

Mutation

deleted amino acid 1-281 and C605S, C631S, C643S …

Promoter

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-N-Flag-ASC1

Plasmid

#75134

Purpose

Expresses N-terminal Flag-tagged ASC1 in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

PYD and CARD domain containing (Pycard Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

F23S (please see depositor comments below)

Promoter

CMV

Available since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-AP1S3

Plasmid

#58292

Purpose

mammalian expression of AP1S3

Depositor

Francesca Capon

Article

Setta-Kaffetzi et al Am J Hum Genet. 2014 May 1;94(5):790-7.

Insert

AP1S3 (AP1S3 Human)

Use

Tags

6xHis and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3_ATP10B-G671R-N865K

Plasmid

#203699

Purpose

expresses ATP10B with the pathogenic mutations G671R and N865K

Depositor

Peter Vangheluwe

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Use

Tags

Expression

Mammalian

Mutation

G671R, N865K

Promoter

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag::UbvG08 P69L, L70V, I44A, deltaGG

Plasmid

#74940

Purpose

Ubiquitin variant with mutations L69P and V70L reverted to wild type (i53-DM mutant)

Depositor

Daniel Durocher

Article

Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d

Insert

Ubiquitin

Use

Tags

Flag

Expression

Mammalian

Mutation

UbvG08 with I44A, P69L, and L70V mutations and no…

Promoter

CMV

Available since

May 13, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-Lyso-mCherry-Rheb(C181S)

Plasmid

#192438

Purpose

Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

Lyso-mCherry-Rheb(C181S) (RHEB Human)

Use

Tags

Full-length LAMP1 and mCherry

Expression

Mammalian

Mutation

Rheb Cys 181 mutated to Ser; abolishes C-terminal…

Promoter

CMV

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PB1-3x-FLAG-B/BRISBANE/60/2008

Plasmid

#187918

Purpose

Expresses C terminal 3xFLAG tagged PB1 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

polymerase basic protein 1

Use

Tags

3xFLAG

Expression

Mutation

Promoter

CMV Promoter

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 NKX6-2-Venus S246A/T252A

Plasmid

#209180

Purpose

Mammalian expression of human NKX6-2 T252D phosphoresistant mutant

Depositor

Federico Herrera

Article

Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023

Insert

NKX6-2 (S246A/T252A) fused with Venus (NKX6-2 Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

NKX6-2 (S246A/T252D)

Promoter

CMV

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_535-888 USP7

Plasmid

#131248

Purpose

Mammalian expression of the USP7 UBL1-3 domains with an N-terminal Myc tag.

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

A mutant form of our pcDNA3.1-N-Myc_535-1102 USP7…

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_535-775 USP7

Plasmid

#131247

Purpose

Mammalian expression of the USP7 UBL1-2 domains with an N-terminal Myc tag.

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

A mutant form of our pcDNA3.1-N-Myc_535-1102 USP7…

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MYO10-HMM-Nanotrap

Plasmid

#87255

Purpose

Expression plasmid to perform NanoSPD 2.0 assays in mammalian cells.

Depositor

Thomas Friedman

Article

Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

Insert

MYO10 (Myo10 Mouse)

Use

Tags

GFP-nanotrap

Expression

Mammalian

Mutation

Myo10 is truncated to contain aa.1-941

Promoter

CMV

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AKAP79-(Ci/Ce)-Epac2-camps

Plasmid

#181956

Purpose

Genetically encoded FRET-based cAMP indicator fused to AKAP79.

Depositor

Jin Zhang

Article

Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

Insert

AKAP79-(Ci/Ce)-Epac2-camps (AKAP5 Human)

Use

Tags

AKAP79, Cerulean (CFP), Citrine (YFP), and HIS ta…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Xpress beta4 1355T

Plasmid

#16040

Depositor

Filippo Giancotti

Article

Nikolopoulos et al Mol Cell Biol. 2005 Jul . 25(14):6090-10

Insert

beta4 (ITGB4 Human)

Use

Tags

6xHis and Xpress

Expression

Mammalian

Mutation

1355T: amino acids 1-1355

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/TO-myc-BirA*-cCE

Plasmid

#112713

Purpose

For tetracycline-inducible expression of myc-BirA*-NES-mCE ΔNLS ("myc-BirA*-cCE") in mammalian cells. For BioID studies.

Depositor

Daniel Schoenberg

Article

Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

Insert

Rngtt (Rngtt Mouse)

Use

Tags

BirA* (BirA R118G), HIV Rev nuclear localization …

Expression

Mammalian

Mutation

Silent mutation at Arg 132 (CGT to CGC), deleted …

Promoter

CMV

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Myc-BirA*-RNF8

Plasmid

#221683

Purpose

Mammalian expression of Myc-tagged BirA*-RNF8 fusion protein for BioID

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

RNF8 (RNF8 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-CUL1 delta 610-615

Plasmid

#19941

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

Insert

cullin 1 delta 610-615 (CUL1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

CUL1 with a ROC1 binding mutant

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-REDD1 R133A/P139A (#626)

Plasmid

#99946

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

REDD1 (DDIT4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

R133A/P139A

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Zeo-BFP-P2A-otPKR-ML

Plasmid

#214365

Purpose

Expression of the Blue Fluorescent Protein - P2A - medium length protein kinase R from chinook salmon

Depositor

Bertrand Collet

Article

Chaumont et al J Immunol. 2024 Jul 26:ji2400076. doi: 1

Insert

BFP-P2A-otPKRML

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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