We narrowed to 6,740 results for: tac

1. pCDNA5/avitag-apex2-v5-fkbp8

   Plasmid

   #154460

   Purpose

   expresses avitag-apex2-v5-fkbp8/biochemical assay

   Depositor

   Hyun-Woo Rhee

   Article

   Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

   Insert

   avitag-apex2-v5-fkbp8 (FKBP8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-F, T7 promoter

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3/flag-pbira(1-g78)-sec61b

   Plasmid

   #154455

   Purpose

   expresses flag-pbira(1-g78)-sec61b/biochemical assay

   Depositor

   Hyun-Woo Rhee

   Article

   Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

   Insert

   flag-pbira(1-g78)-sec61b (SEC61B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLCHKO_TK1_HPRT1_Lb_4

   Plasmid

   #155062

   Purpose

   Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a (CHyMErA system)

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFlare9A-Clip2E9 Mutant

   Plasmid

   #209575

   Purpose

   minigene construct for Clip2 E9 alternative splicing, potential PTBP2 binding sites are deleted

   Depositor

   Sika Zheng

   Article

   Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

   Insert

   Clip2 (Clip2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   ACGCGTTTCTGAATTCTTCTAACTGCCCTCAAATGCACGGTGGCATGTG…

   Promoter

   CMV

   Available since

   March 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1 puro shCALM2-1

   Plasmid

   #193699

   Purpose

   Constitutive lentiviral expression of CALM2 shRNA

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   CALM2 (CALM2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-mCherry-Rorb sesRNA-2a-msFlag-2a-tTA2-WPRE

   Plasmid

   #239029

   Purpose

   Expression of mCherry, sesRNA in mammalian cells, with msFlag and tTA2 as efRNA.

   Depositor

   Josh Huang

   Article

   Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

   Insert

   sesRNA for mouse Rorb (Rorb Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-mCherry-Satb2 sesRNA-2a-msFlag-2a-tTA-WPRE

   Plasmid

   #239028

   Purpose

   Expression of mCherry, sesRNA in mammalian cells, with msFlag and tTA2 as efRNA.

   Depositor

   Josh Huang

   Article

   Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

   Insert

   sesRNA for mouse Satb2 (Satb2 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. Adeno FT

   Plasmid

   #101824

   Purpose

   Adenovirus for the expression of gRNAs targeting intron 17 of murine Fgfr3 and intron 6 of murine Tacc3

   Depositor

   Andrea Ventura

   Article

   Cook et al Nat Commun. 2017 Jul 11;8:15987. doi: 10

   Insert

   U6_sgRNA(Fgfr3)_U6_sgRNA(Tacc3)_CBh_FLAG-Cas9 (Fgfr3, Tacc3 Mouse)
   

   Use

   Adenoviral

   Tags

   FLAG-Cas9

   Expression

   Mutation

   Promoter

   U6 and CBh

   Available since

   Nov. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pGEX Emt-SH2

   Plasmid

   #46424

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Emt (ITK Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 223-346

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX SHIP1-SH2

    Plasmid

    #46492

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SHIP1 (INPP5D Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1-118

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX SHP-1(NC)-SH2

    Plasmid

    #46496

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SHP-1(NC) (PTPN6 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1-221

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX6P2-p53 (1-90) (72R)

    Plasmid

    #62081

    Purpose

    Expression of human p53 (TAD residues 1-90) (72R) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains TAD/PP residues 1-90, 72R

    Promoter

    tac

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

13. AFDN_PDZ_1

    Plasmid

    #103938

    Purpose

    Protein expression and purification of TRITHORAX PDZ domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    AFDN (AFDN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains AA983-1110 of AFDN

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX6P2-MDM2 (17-125)

    Plasmid

    #62063

    Purpose

    Expression of human MDM2 (17-125) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    MDM2 (MDM2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains residues 17-125

    Promoter

    tac

    Available since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX6P1‐hsRNASEH2BCA

    Plasmid

    #108692

    Purpose

    For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzyme

    Depositor

    Martin Reijns, Andrew Jackson

    Article

    Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

    Inserts

    RNASEH2B (RNASEH2B Human)
    RNASEH2C (RNASEH2C Human)
    RNASEH2A (RNASEH2A Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Shine Dalgarno sequence upstream of start codon a…

    Promoter

    tac

    Available since

    April 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pERM2-nhpSer

    Plasmid

    #201922

    Purpose

    Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.

    Depositor

    Richard Cooley, Ryan Mehl

    Article

    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

    Inserts

    SepRS(2)
    Sep-tRNA v2.0
    SerB
    EF-Sep
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    E412P, E414F, T417K, P495M, I496W, F529S and H67R…

    Promoter

    GlnS (constitutive), OXB20 (constitutive), lpp (c…

    Available since

    June 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX Src-SH2

    Plasmid

    #46510

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Src (SRC Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 142-252

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-6p1-GST-ArcΔCTD

    Plasmid

    #119878

    Purpose

    Bacterial Expression construct for the production and purification of Arc missing C-terminal Domain

    Depositor

    Jason Shepherd

    Article

    Pastuzyn et al Cell. 2018 Mar 22;173(1):275. doi: 10.10

    Insert

    Activity Regulated Cytoskeletal Protein (Arc Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Deletion of nt 832-1110

    Promoter

    tac

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX6P1-3xMBT_D355N

    Plasmid

    #46988

    Purpose

    Inducible expression of 3xMBT (D355N) from L3MBTL1 as a GST fusion protein in E.coli

    Depositor

    Or Gozani

    Article

    Moore et al Mol Cell. 2013 May 9;50(3):444-56. doi:

    Insert

    Triple MBT domain from human L3MBTL1 (L3MBTL1 Human)
    

    Use

    Tags

    GST and PreScission cleavage site

    Expression

    Bacterial

    Mutation

    Mutation aspartic acid 355 to asparagine (D355N);…

    Promoter

    tac

    Available since

    Sept. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX6p1-Rbck1

    Plasmid

    #63135

    Purpose

    Expression of human RBCK1/HOIL-1L

    Depositor

    Titia Sixma

    Article

    Smit et al EMBO J. 2012 Oct 3;31(19):3833-44. doi:

    Insert

    HOIL-1L (RBCK1 Synthetic, Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Synthetic based on human protein sequence

    Promoter

    tac

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only