We narrowed to 6,570 results for: tac

1. pAAV-hSyn-mCherry-Rorb sesRNA-2a-msFlag-2a-tTA2-WPRE

   Plasmid

   #239029

   Purpose

   Expression of mCherry, sesRNA in mammalian cells, with msFlag and tTA2 as efRNA.

   Depositor

   Josh Huang

   Article

   Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

   Insert

   sesRNA for mouse Rorb (Rorb Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-mCherry-Satb2 sesRNA-2a-msFlag-2a-tTA-WPRE

   Plasmid

   #239028

   Purpose

   Expression of mCherry, sesRNA in mammalian cells, with msFlag and tTA2 as efRNA.

   Depositor

   Josh Huang

   Article

   Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

   Insert

   sesRNA for mouse Satb2 (Satb2 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. Adeno FT

   Plasmid

   #101824

   Purpose

   Adenovirus for the expression of gRNAs targeting intron 17 of murine Fgfr3 and intron 6 of murine Tacc3

   Depositor

   Andrea Ventura

   Article

   Cook et al Nat Commun. 2017 Jul 11;8:15987. doi: 10

   Insert

   U6_sgRNA(Fgfr3)_U6_sgRNA(Tacc3)_CBh_FLAG-Cas9 (Fgfr3, Tacc3 Mouse)
   

   Use

   Adenoviral

   Tags

   FLAG-Cas9

   Expression

   Mutation

   Promoter

   U6 and CBh

   Available since

   Nov. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

4. pEMS1686

   Plasmid

   #29291

   Purpose

   Expression of the reporter gene was not detected in the brain and eye using LacZ.

   Depositor

   Elizabeth Simpson

   Article

   Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

   Insert

   Ple214 (TAC1 Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   Intron-LacZ-NLS

   Expression

   Mutation

   Promoter

   Available since

   June 10, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pGEX Emt-SH2

   Plasmid

   #46424

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Emt (ITK Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 223-346

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pERM2-nhpSer

   Plasmid

   #201922

   Purpose

   Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.

   Depositor

   Richard Cooley, Ryan Mehl

   Article

   Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

   Inserts

   SepRS(2)
   Sep-tRNA v2.0
   SerB
   EF-Sep
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   E412P, E414F, T417K, P495M, I496W, F529S and H67R…

   Promoter

   GlnS (constitutive), OXB20 (constitutive), lpp (c…

   Available since

   June 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX6P2-MDM2 (17-125)

   Plasmid

   #62063

   Purpose

   Expression of human MDM2 (17-125) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

   Insert

   MDM2 (MDM2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   contains residues 17-125

   Promoter

   tac

   Available since

   Feb. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX6P1‐hsRNASEH2BCA

   Plasmid

   #108692

   Purpose

   For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzyme

   Depositor

   Martin Reijns, Andrew Jackson

   Article

   Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

   Inserts

   RNASEH2B (RNASEH2B Human)
   RNASEH2C (RNASEH2C Human)
   RNASEH2A (RNASEH2A Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Shine Dalgarno sequence upstream of start codon a…

   Promoter

   tac

   Available since

   April 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-6p1-GST-ArcΔCTD

   Plasmid

   #119878

   Purpose

   Bacterial Expression construct for the production and purification of Arc missing C-terminal Domain

   Depositor

   Jason Shepherd

   Article

   Pastuzyn et al Cell. 2018 Mar 22;173(1):275. doi: 10.10

   Insert

   Activity Regulated Cytoskeletal Protein (Arc Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Deletion of nt 832-1110

   Promoter

   tac

   Available since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX6p1-Rbck1

    Plasmid

    #63135

    Purpose

    Expression of human RBCK1/HOIL-1L

    Depositor

    Titia Sixma

    Article

    Smit et al EMBO J. 2012 Oct 3;31(19):3833-44. doi:

    Insert

    HOIL-1L (RBCK1 Synthetic, Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Synthetic based on human protein sequence

    Promoter

    tac

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pGEX Src-SH2

    Plasmid

    #46510

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Src (SRC Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 142-252

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX6P1-3xMBT_D355N

    Plasmid

    #46988

    Purpose

    Inducible expression of 3xMBT (D355N) from L3MBTL1 as a GST fusion protein in E.coli

    Depositor

    Or Gozani

    Article

    Moore et al Mol Cell. 2013 May 9;50(3):444-56. doi:

    Insert

    Triple MBT domain from human L3MBTL1 (L3MBTL1 Human)
    

    Use

    Tags

    GST and PreScission cleavage site

    Expression

    Bacterial

    Mutation

    Mutation aspartic acid 355 to asparagine (D355N);…

    Promoter

    tac

    Available since

    Sept. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX6P2-p53 (1-90) (72R)

    Plasmid

    #62081

    Purpose

    Expression of human p53 (TAD residues 1-90) (72R) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains TAD/PP residues 1-90, 72R

    Promoter

    tac

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pGEX-6P-1-INTS3-FL

    Plasmid

    #128415

    Purpose

    Express INTS3 full-length in E. coli with a GST tag (N-terminal)

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

    Insert

    INTS3 (INTS3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    full-length

    Promoter

    tac promoter

    Available since

    Aug. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX6P1‐hsRNASEH2BCA(D34A/D169A)

    Plasmid

    #108693

    Purpose

    For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A (with D34A and D169A catalytic site mutations) for purification of the RNase H2 trimeric enzyme

    Depositor

    Martin Reijns, Andrew Jackson

    Article

    Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

    Inserts

    RNASEH2B (RNASEH2B Human)
    RNASEH2C (RNASEH2C Human)
    RNASEH2A-D34A/D169A
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Shine Dalgarno sequence upstream of start codon, …

    Promoter

    tac

    Available since

    April 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX4T1_GST-PLproCS-MBP

    Plasmid

    #169195

    Purpose

    To express a PLpro substrate (GST-PLproCS-MBP) in E. coli

    Depositor

    John Diffley

    Article

    Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

    Insert

    GST-PLproCS-MBP
    

    Use

    Tags

    GST-MBP fusion (PLpro-cleavable) and His6 (intern…

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX PLCg1(NC)-SH2

    Plasmid

    #46471

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    PLCg1(NC) (PLCG1 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 542-759

    Promoter

    Tac

    Available since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGex-4T-2_LC3A

    Plasmid

    #73946

    Purpose

    For recombinant expression of human LC3A/MAP1LC3A in E. coli

    Depositor

    Dieter Willbold

    Article

    Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

    Insert

    LC3A (MAP1LC3A Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pGEX FynA-SH2

    Plasmid

    #46429

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    FynA (FYN Human)
    

    Use

    Tags

    AviTag, GST, and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 142-251

    Promoter

    Tac

    Available since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX Grb2-SH2

    Plasmid

    #46440

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Grb2 (GRB2 Human)
    

    Use

    Tags

    GST and Thrombin site

    Expression

    Bacterial

    Mutation

    contains amino acids 58-159

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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