-
Plasmid#194176PurposeExpresses first 1200 nt of lncRNA HIF1a-AS1 (unspliced)DepositorInsertHIF1a-AS1 (HIF1A-AS1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-PIK3CA H1047R-WPRE-UbC-Emerald
Plasmid#203798PurposeLentiviral vector expressing human PIK3CA H1047R under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsExpressionMammalianMutationH1047RPromoterAvailable sinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple Gaq
Plasmid#196058PurposeEncodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple GaZ
Plasmid#196053PurposeEncodes a G alpha subunit (GNAZ) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pME-QF2
Plasmid#83307Purposecodes for QF2 (truncated QF) in a gateway middle entry backbone.DepositorInsertQF
UseTagsExpressionMutationPromoterAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-mTagBFP2
Plasmid#87254PurposeLentiviral fluorescent reporter using the neuronal cell type-specific promoter of the CaM Kinase II alpha gene.DepositorInsertmTagBFP2
UseLentiviralTags6xHisExpressionMutationPromoterCaMKIIaAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK275
Plasmid#73363PurposeEncodes Crepis alpina delta-8 sphingolipid desaturase (SLD1)DepositorInsertdelta-8 sphingolipid desaturase
UseTagsExpressionYeastMutationmissing residues 1-6PromoterGAL1Available sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
RCAS IkB K->A
Plasmid#12408DepositorInsertIkB alpha K->A (Nfkbia Mouse)
UseRetroviralTagsExpressionMammalianMutationLysines 21, 22, and 67 are mutated to alanine. (T…PromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLenti4/V5-DEST-P85
Plasmid#40219DepositorInsertP85 alpha (PIK3R1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
-
-
-
pMS18
Plasmid#225681PurposeConfers puromycin resistance to Salpingoeca rosettaDepositorInsertpEFL-Pac-Actin_3'UTR
UseTagsExpressionMutationPromoterAvailable sinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJK369
Plasmid#73594PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitopeDepositorInsertdelta-12 fatty acid acetylenase
UseTagsFLAG tagExpressionYeastMutationPromoterGAL10Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK137
Plasmid#73313PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with c-myc tag and His6-tagDepositorInsertdelta-12 fatty acid acetylenase
UseTagsHis6 and c-mycExpressionYeastMutationsynthetic genePromoter5'-AOX1Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK108
Plasmid#73312PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2/synthetic)DepositorInsertdelta-12 fatty acid acetylenase
UseTagsExpressionBacterialMutationsynthetic genePromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKPY737
Plasmid#72673PurposeEncodes Thr412Gly-CePheRS Alpha Subunit (Gateway Entry Clone)DepositorInsertThr412Gly-CePheRS Alpha Subunit::C. elegans CEOP5428 Intercistronic Region::HA-GFP [From pPD95.75 (+118 to +1000)] in pDONR221
UseGateway entry cloneTagsExpressionMutationThr412Gly in CePheRS Alpha SubunitPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
4xnrUAS:GFP
Plasmid#61373Purpose4xnrUAS driving GFPDepositorInsert4xnr UAS:GFP
UseTagsExpressionMutationPromoterAvailable sinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn S129L
Plasmid#36062DepositorInsertalpha-synuclein S129L (SNCA Human)
UseTagsExpressionBacterialMutationS129LPromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn S129I
Plasmid#36061DepositorInsertalpha-synuclein S129I (SNCA Human)
UseTagsExpressionBacterialMutationS129IPromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only