Addgene

We narrowed to 22,251 results for: Sele

Showing: 2281 - 2310 of 22251 results

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pJAK184.tetR.PT5-3.dCas9

Plasmid

#232482

Purpose

optimized ahTet inducible dCas9 for insertion into C. difficile genome

Depositor

James Collins

Article

Chua et al Microbiol Resour Announc. 2025 Apr 28:e0

Insert

Catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

TetR-PT5-3

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT221: pR6K-Donor(ZooTsy)_H1:12_E1:135_E2:20_H2:12

Plasmid

#200874

Purpose

ZooTsy donor harboring KanR cargo.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT401: pHelper(AjTn6022)_QF-separated_TniQ-K361D

Plasmid

#200861

Purpose

Inducible expression of AjTn6022 proteins.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022

Use

Tags

Expression

Bacterial

Mutation

TniQ-K361D

Promoter

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT400: pHelper(AjTn6022)_QF-separated_TniQ-K348D

Plasmid

#200860

Purpose

Inducible expression of AjTn6022 proteins.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022

Use

Tags

Expression

Bacterial

Mutation

TniQ-K348D

Promoter

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP103: pTarget(CyCAST)

Plasmid

#200849

Purpose

Target plasmid containing CyPSP1 and attachement site for CyCAST.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Inserts

PAM for CyCAST + CyPSP1
tRNA-Leu

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP270: pHelper(CyCAST)_Shuffled-spacer_ΔTnsD

Plasmid

#200845

Purpose

Inducible expression of CyCAST proteins (except TnsD) with control crRNA for CyPSP1.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

CyCAST

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP124: pDonor(CyCAST)_0.5kb-cargo

Plasmid

#200848

Purpose

CyCAST donor harboring non-functional cargo.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

CyCAST

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP267: pHelper(CyCAST)_PSP1_ΔTniQ

Plasmid

#200842

Purpose

Inducible expression of CyCAST proteins (except TniQ) with crRNA targeting CyPSP1.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

CyCAST

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSIP269: pHelper(CyCAST)_PSP1_ΔTnsD

Plasmid

#200844

Purpose

Inducible expression of CyCAST proteins (except TnsD) with crRNA targeting CyPSP1.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

CyCAST

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSIP116: pHelper(CyCAST)_PSP1

Plasmid

#200840

Purpose

Inducible expression of CyCAST proteins with crRNA targeting CyPSP1.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

CyCAST

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSIP122: pHelper(CyCAST)_Shuffled-spacer

Plasmid

#200841

Purpose

Inducible expression of CyCAST proteins with control crRNA for CyPSP1.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

CyCAST

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-7xCGCGCG

Plasmid

#233625

Purpose

Expression of reporter gene with 7xCGCGCG motifs

Depositor

Shintaro Iwasaki

Article

Saito et al Nat Commun. 2024 Sep 2;15(1):7418. doi:

Insert

7xCGCGCG

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-7xUCUCUC

Plasmid

#233623

Purpose

Expression of reporter gene with 7xUCUCUC motifs

Depositor

Shintaro Iwasaki

Article

Saito et al Nat Commun. 2024 Sep 2;15(1):7418. doi:

Insert

7xUCUCUC

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-7xUGUGUG

Plasmid

#233624

Purpose

Expression of reporter gene with 7xUGUGUG motifs

Depositor

Shintaro Iwasaki

Article

Saito et al Nat Commun. 2024 Sep 2;15(1):7418. doi:

Insert

7xUGUGUG

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pGFP-AUA-IRES-mCherry

Plasmid

#222116

Purpose

Start codon reporter with mutated start codon

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Tags

Expression

Mutation

AUA start codon

Promoter

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP-AGC-IRES-mCherry

Plasmid

#222119

Purpose

Start codon reporter with mutated start codon

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Tags

Expression

Mutation

AGC start codon

Promoter

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGFP-CUG-IRES-mCherry

Plasmid

#222110

Purpose

Start codon reporter with mutated start codon

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Tags

Expression

Mutation

CUG start codon

Promoter

Available since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGFP-ACG-IRES-mCherry

Plasmid

#222113

Purpose

Start codon reporter with mutated start codon

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Tags

Expression

Mutation

ACG start codon

Promoter

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGFP-UUG-IRES-mCherry

Plasmid

#222112

Purpose

Start codon reporter with mutated start codon

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Tags

Expression

Mutation

UUG start codon

Promoter

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGFP-AUU-IRES-mCherry

Plasmid

#222118

Purpose

Start codon reporter with mutated start codon

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Tags

Expression

Mutation

AUU start codon

Promoter

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGFP-AGG-IRES-mCherry

Plasmid

#222115

Purpose

Start codon reporter with mutated start codon

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Tags

Expression

Mutation

AGG start codon

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGFP-AUC-IRES-mCherry

Plasmid

#222117

Purpose

Start codon reporter with mutated start codon

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

GFP-IRES-mCherry

Use

Lentiviral

Tags

Expression

Mutation

AUC start codon

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBa.KIF13B

Plasmid

#224413

Purpose

Mammalian expression of Kif 13b

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

Kif 13b (Kif13b Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Chicken Beta actin

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pBa.KIF13A

Plasmid

#224408

Purpose

Mammalian expression of Kif 13a

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

Kif 13a (Kif13a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Chicken Beta actin

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pBa.Mark2-3myc

Plasmid

#224416

Purpose

Mammalian expression of MARK2

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

MARK2 (MARK2 Human)

Use

Tags

3myc

Expression

Mammalian

Mutation

Promoter

Chicken Beta actin

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pSD118A

Plasmid

#218140

Purpose

Expresses AviTag-6xHis-MBP-TEV-SD0(ZF2-3) for protein purification from E. coli

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD0_ZF2-3

Use

Tags

AviTag-6xHis-MBP-TEV-(GGGGS)2

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pSD068F

Plasmid

#218137

Purpose

Lentiviral vector expressing evolved degron SD36 fused to eGFP with mCherry control.

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD36-eGFP-IRES2-mCherry

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSD057C

Plasmid

#218136

Purpose

For hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to evolved ZF degron SD36.

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD36

Use

Tags

rpoZ

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSD113A

Plasmid

#218133

Purpose

For hybrid circuit activation. Expresses E. coli RNAP omega subunit fused to evolved degron SD56

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD56

Use

Tags

rpoZ

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJM154

Plasmid

#218121

Purpose

Hybrid circuit displaying FKBP12 bait and driving expression of luxAB

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

FKBP12
luxAB

Use

Luciferase

Tags

434cI_RR69

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge