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pJ291_WT_med

Plasmid

#128856

Purpose

Medium copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pDM_noro_S6_lacZA

Plasmid

#118817

Purpose

T7 RNAP-driven expression of norovirus sense orientation toehold switches (S6) with a lacZ alpha subunit reporter.

Depositor

Alexander Green

Article

Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

Insert

Norovirus toehold switch S6

Expression

Bacterial

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pVH234-Tier1-PmPGK1-tTA

Plasmid

#169574

Purpose

Tier-1 vector encoding PmPGK1-driven tTA expression (PmPGK1-tTA-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled transactivator

Expression

Mammalian

Promoter

PPGK

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-mPlexinD1

Plasmid

#16424

Depositor

Jonathan Epstein

Article

Gitler et al Dev Cell. 2004 Jul . 7(1):107-16.

Insert

Plexin D1 (Plxnd1 Mouse)

Use

In situ template

Mutation

None

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only
pCMV-FKBP-mTagBFP2-MCS

Plasmid

#178864

Purpose

Mammalian expression of a protein of interest fused to the C-terminus of FKBP-mTagBFP2

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

FKBP-mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pNM3649

Plasmid

#154110

Purpose

miniMos dual component RMCE landing site vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

loxP::SEC::loxP::rpl-28p::FRT::GFP::his-58::unc-54 3'::FRT3

Use

Minimos

Promoter

not applicable

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pKS2311

Plasmid

#37835

Depositor

Gary Silverman

Article

Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

Insert

Pnhx-2cpl-1W32A;Y35A::YFP (cpl-1 Nematode)

Tags

YFP

Expression

Worm

Mutation

W32A;Y35A

Promoter

nhx-2

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pPdiso4E-ISH

Plasmid

#61701

Purpose

Plum eIF(iso)4E ihp-RNA construct

Depositor

Lining Tian

Article

Wang et al PLoS One. 2013;8(1):e50627. doi: 10.1371

Insert

PdeIF(iso)4E

Use

RNAi

Promoter

35S

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-K-I-CAAX

Plasmid

#41642

Purpose

Catalytically active control, intersectin Dbl homology (DH) domain fused to wild-type Dronpa

Depositor

Michael Lin

Article

Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

Insert

Dronpa145K-IntersectinDH-CAAX

Tags

CAAX box

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pRS426-GFP-Num1-PH

Plasmid

#71374

Purpose

For analysis of PH domain localization in yeast

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

Num1-PH

Tags

EGFP

Expression

Yeast

Mutation

Pleckstrin homology (PH) domain; aa2563-2692

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pJEC578

Plasmid

#174366

Purpose

dCas9 for modular CRISPRa. SYNZIP-dCas9. Expresses dCas9 with a SYNZIP18 domain fused to the N terminus.

Depositor

James Chappell

Article

Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

Insert

dCas9 fused to SYNZIP18

Expression

Bacterial

Promoter

J23150

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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CMVTCRb_candidate2

Plasmid

#164998

Purpose

Expression of candidate TCR #2 TCRbeta cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRB (TRB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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CMVTCRa_candidate2

Plasmid

#164997

Purpose

Expression of candidate TCR #2 TCRalpha cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRAV (TRA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-rbNHERF1

Plasmid

#31636

Depositor

Edward Weinman

Article

Voltz et al J Biol Chem. 2007 Nov 16. 282(46):33879-

Insert

solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)

Tags

eGFP

Expression

Mammalian

Promoter

cmv

Available Since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti pTERT sgRNA2(MS2) mCherry

Plasmid

#171055

Purpose

Targeting of Vp64-dCas9 activation system to hTERT promoter

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

pTERT sgRNA2(MS2) mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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mEos3.2-MCPB2-C-18

Plasmid

#57473

Purpose

Localization: Actin Capping Protein , Excitation: 507 / 572, Emission: 516 / 580

Depositor

Michael Davidson

Insert

MCPB2 (Capzb Mouse)

Tags

mEos3.2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pHFX-cas3UD

Plasmid

#174385

Purpose

Plasmids for gene knockout were constructed based on suicide plasmid pHFX

Depositor

Jing Han

Article

Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

Insert

Cas3UD

Use

Archaeal gene knockout vector for haloferax medit…

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMPRMA38

Plasmid

#172937

Purpose

pQE30 encoding Wzz mutant i92

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i92 mutant gene

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pMPRMA45

Plasmid

#172934

Purpose

pQE30 encoding Wzz mutant i32

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i32 mutant gene

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pMPRMA48

Plasmid

#172941

Purpose

pQE30 encoding Wzz mutant i131

Depositor

Renato Morona

Article

Papadopoulos et al J Bacteriol. 2010 Jul;192(13):3385-93. d

Insert

Shigella flexneri wzz i131 mutant gene

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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