We narrowed to 9,250 results for: CAG

1. pPyCAG-NE-mKate2-IRES-Hph

   Plasmid

   #183616

   Purpose

   Mammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered mKate2 (NLS-mKate2-EmerinTMD). Confers hygromycin B resistance.

   Depositor

   Sally Lowell

   Article

   Blin et al PLoS Biol. 2019 Aug 9;17(8):e3000388. do

   Insert

   mKate2
   

   Tags

   Human EMD transmembrane domain and NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-PB1-AG(Y63L)-LOV2

   Plasmid

   #178876

   Purpose

   Mammalian expression of PB1-AzamiGreen(Y63L)-LOV2

   Depositor

   Shinya Tsukiji

   Article

   Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

   Insert

   PB1-AzamiGreen(Y63L)-LOV2
   

   Expression

   Mammalian

   Mutation

   AzamiGreen: Y63L

   Promoter

   CAG

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-attBr-ZsGreen-attP

   Plasmid

   #51265

   Purpose

   Bxb1-responsive fluorescent reporter plasmid containing reverse complementary ZsGreen flanked by Bxb1 attB/P recognition sites in head-to-head orientation

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   attBr-ZsGreen-attP
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CAG-soCoChR-GFP

   Plasmid

   #107710

   Purpose

   A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. CAG promoter

   Depositor

   Edward Boyden

   Article

   Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

   Insert

   soCoChR-GFP
   

   Use

   AAV

   Tags

   EGFP and KA2

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-MBP-ATG9-P834A

   Plasmid

   #188082

   Purpose

   Expresses human ATG9-P834A mutant in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG9 (ATG9A Human)
   

   Tags

   MBP tag

   Expression

   Mammalian

   Mutation

   P834A

   Promoter

   CMV

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-RaichuEV-Rac1(T17N)

   Plasmid

   #164902

   Purpose

   Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation T17N in the Rac1 domain

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   RaichuEV-Rac1 (RAC1 Human)
   

   Expression

   Mammalian

   Mutation

   T17N mutation in the Rac1 domain which make the s…

   Available Since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. GLI1_pLENTI-CAG-IRES-GFP

   Plasmid

   #176992

   Purpose

   Mammalian lentiviral expression vector encoding GLI1

   Depositor

   Roger Reeves

   Article

   Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

   Insert

   GLI1 (GLI1 Human)
   

   Use

   Lentiviral

   Promoter

   CAG

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pf CAG mc2 IRES blast

   Plasmid

   #206235

   Purpose

   For expressing cloned exons as circular RNA.

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pf CAG mc2 IRES puro

   Plasmid

   #206233

   Purpose

   For expressing cloned exons as circular RNA.

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB CAG-sfGFP-iCre-ERT2

    Plasmid

    #137856

    Purpose

    CAG-sfGFP-iCre-ERT2 piggyBac transposon

    Depositor

    Mario Capecchi

    Insert

    sfGFP-iCre-ERT2
    

    Use

    Cre/Lox; Piggybac

    Expression

    Mammalian

    Available Since

    Nov. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB CAG-F-bpA-3xSV40pA-F-td-sfGFP

    Plasmid

    #133573

    Purpose

    CAG tdGFP FLP/FRT reporter piggyBac transposon (bpA 3x SV40pA stop)

    Depositor

    Mario Capecchi

    Insert

    Tandem dimer superfolder GFP
    

    Use

    Flp/frt

    Expression

    Mammalian

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-Bgh-GFP-Farpl

    Plasmid

    #115501

    Purpose

    expression of rat Farp1

    Depositor

    Thomas Biederer

    Article

    Cheadle et al J Cell Biol. 2012 Dec 10;199(6):985-1001

    Insert

    Farp1 (Farp1 Rat)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    chicken beta-actin

    Available Since

    Dec. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. CAG.3xHalo-GFP-SPC25

    Plasmid

    #176206

    Purpose

    Kinetochore anchor for TMP-Halo dimerization system

    Depositor

    Michael Lampson

    Article

    Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

    Insert

    3xHalo-GFP-SPC25
    

    Expression

    Mammalian

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKLD-CAG-DYSF-CFL

    Plasmid

    #131457

    Purpose

    Encodes Dysferlin protein (Therapeutic gene) along with Follistatin protein to cure LGMD2B along with luciferase enzyme for in vivo live imaging

    Depositor

    Michele Calos

    Article

    Guha et al Mol Ther Methods Clin Dev. 2019 Oct 14;1

    Insert

    DYSF and Follistatin (DYSF, FST Human)
    

    Expression

    Bacterial and Mammalian

    Mutation

    R15C (in DYSF) and Q2579H (in CFL)

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAGGS-SARS2-S-FKO (NC-flag)

    Plasmid

    #159363

    Purpose

    Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at N- and C-termini.

    Depositor

    Hyeryun Choe, Michael Farzan

    Article

    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

    Insert

    SARS-CoV-2 S protein (S SARS-CoV-2)
    

    Tags

    Flag and Flag

    Expression

    Mammalian

    Mutation

    Furin-cleavage site mutation

    Promoter

    CMV

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. CAG-Brn2-DBD-EnR

    Plasmid

    #19714

    Depositor

    Connie Cepko

    Article

    Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

    Insert

    Fly engrailed 36240 (Pou3f2, en Fly, Mouse)
    

    Expression

    Mammalian

    Mutation

    mouse Brn2 DNA binding domain-Drosophila engraile…

    Available Since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pCAGGS-SARS2-S-G614 (NC-flag)

    Plasmid

    #156419

    Purpose

    Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

    Depositor

    Hyeryun Choe, Michael Farzan

    Article

    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

    Insert

    SARS-CoV-2 S protein (S SARS CoV2)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D614G

    Promoter

    CMV

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB CAG-L-bpA-2xSV40pA-L-td-sfGFP

    Plasmid

    #133576

    Purpose

    CAG tdGFP cre/lox reporter piggyBac transposon (bpA 2x SV40pA stop)

    Depositor

    Mario Capecchi

    Insert

    Tandem dimer superfolder GFP
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS-Flag-hsDicer (E1564A)

    Plasmid

    #41587

    Depositor

    Phil Sharp

    Article

    Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

    Insert

    hsDicer (DICER1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    E1564A

    Promoter

    CMV

    Available Since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pCAG-LbCas12a-Plus-mCherry

    Plasmid

    #191230

    Purpose

    To express the LbCas12a nuclease variant "LbCas12a-Plus" that enhanced the editing activity and specificity

    Depositor

    Zhili Rong

    Article

    Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

    Insert

    LbCas12a-Plus-P2A-mCherry
    

    Use

    CRISPR

    Mutation

    D156R, R883K, R887A

    Available Since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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