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Addgene

We narrowed to 8,839 results for: Pol

Showing: 1521 - 1540 of 8839 results
  1. pSN-A112C-1A32

    Plasmid
    #191241
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.
    Depositor
    Insert
    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Use
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Feb. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. pSN-A112C-1A31

    Plasmid
    #191242
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.
    Depositor
    Insert
    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Use
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. pSN-A112C-CP8B1

    Plasmid
    #191240
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.
    Depositor
    Insert
    nuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Use
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q-P70C

    Plasmid
    #114629
    Purpose
    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P70C cysteine mutations and a 49 polyQ repeat
    Depositor
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    A2C, P70C; 49 polyQ repeat
    Promoter
    Available since
    Oct. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  5. pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q-A60C

    Plasmid
    #114628
    Purpose
    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 49 polyQ repeat
    Depositor
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    A2C, A60C; 49 polyQ repeat
    Promoter
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
  6. pTWIN1-His6-Ssp-Htt2-90(A2C)-23Q-P80C

    Plasmid
    #114614
    Purpose
    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 23 polyQ repeat
    Depositor
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    A2C, P80C; 23 polyQ repeat
    Promoter
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
  7. pTWIN1-His6-Ssp-Htt2-90(A2C)-43Q-P70C

    Plasmid
    #114624
    Purpose
    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P70C cysteine mutations and a 43 polyQ repeat
    Depositor
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    A2C, P70C; 43 polyQ repeat
    Promoter
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
  8. pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q-P90C

    Plasmid
    #114630
    Purpose
    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 49 polyQ repeat
    Depositor
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    A2C, P90C; 49 polyQ repeat
    Promoter
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
  9. pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-P90C

    Plasmid
    #114610
    Purpose
    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 15 polyQ repeat
    Depositor
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    A2C, P90C; 15 polyQ repeat
    Promoter
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
  10. pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-P70C

    Plasmid
    #114609
    Purpose
    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P70C cysteine mutations and a 15 polyQ repeat
    Depositor
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    A2C, P70C; 15 polyQ repeat
    Promoter
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
  11. pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-P90C

    Plasmid
    #114620
    Purpose
    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P90C cysteine mutations and a 37 polyQ repeat
    Depositor
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    A2C, P90C; 37 polyQ repeat
    Promoter
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 1521 - 1540 of 8839 results