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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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171,035 results

Showing: 7771 - 7780 of 171035 results

  3. pJR85

    Plasmid
    #140095
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA with cs1 in stem loop 2 of the constant region
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140095-plasmid-map-sequence-id-292013

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  7. MAT2B

    Plasmid
    #39080
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAT2B (MAT2B Human)
    Tags
    Tev His Flag
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  8. pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP

    Plasmid
    #85165
    Purpose
    Provides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.
    Depositor
    Robin Dowell
    Article
    Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71
    Insert
    EcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
    Tags
    AmilCP and RTX
    Expression
    Bacterial
    Promoter
    J23100 promoter
    Available Since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    85165-plasmid-map-sequence-id-354765

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Showing: 7771 - 7780 of 171035 results