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Plasmid#220446PurposeSplit intein version of Trojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorInsertCfaN-Gal4-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiT-GEM(2)
Plasmid#220448PurposeSplit intein version of Trojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorInsertCfaN-Gal4-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiT-GEM(1)
Plasmid#220447PurposeSplit intein version of Trojan-Gal4 Expression Module in Phase 1. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorInsertCfaN-Gal4-CfaC
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
INT_Cargo_T7_gfp
Plasmid#212136PurposeCargo plasmid for integrating PT7 expression cassette of green fluorescent protein in genome.Plasmid can be removed by incubating cells at 37 C.DepositorInsertgreen fluorescent protein
UseTagsExpressionBacterialMutationPromoterPT7Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
INT_Cargo_T7_CbFDH
Plasmid#212140PurposeCargo plasmid for integrating PT7 expression cassette of formate dehydrogenase in genome. Plasmid can be removed by incubating cells at 37 C.DepositorInsertformate dehydrogenase
UseTagsExpressionBacterialMutationPromoterPT7Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti-Cas9-sgHPRT1
Plasmid#196713PurposeCRISPR-KO. WT-SpCas9 and sgRNA targeting HPRT1. Editing-competent cells can be selected with 6-TGDepositorInsertCas9-T2A-BSD-U6-sgHPRT1
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSExpressionMutationPromoterEF1a/hU6Available sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
1056D
Plasmid#183132PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii bTub, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[bTub]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056E
Plasmid#183133PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii dsx, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[dsx]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056F
Plasmid#183134PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii tra, Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[tra]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
FKBP-EGFP-RanGAP
Plasmid#175236PurposeBacterial expression and purification, high affinity SUMOylation substrate that can recruited to FRB with rapamycinDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj12a
Plasmid#173141PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj12a (kcnj12a Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-kcnj16
Plasmid#173146PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj16a (kcnj16 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationStop codon removedPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRBad-C-wtBlc
Plasmid#168257PurposeC-fragment. This plasmid can be co-transformed with either Plasmid 168259: pET11a-N-wtBlc or Plasmid 168472: pET11a-N-DiB2 to obtain wtBlc‐split or DiB2‐split protein, correspondinglyDepositorInsertC-fragment of wtBlc
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
shCDK4(1)
Plasmid#73550PurposeshRNA(1) against CDK4DepositorInsertshRNA against CDK4
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
shCDK4(2)
Plasmid#73551PurposeshRNA(2) against CDK4DepositorInsertshRNA against CDK4
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
GST-ZNF668
Plasmid#45042DepositorInsertZNF668 (ZNF668 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUC57-LbNOX
Plasmid#75285PurposeThis plasmid contains human codon optimized sequence of LbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.DepositorInsertLbNOX
UseTagsC-terminal FLAG tag with linkerExpressionMutationHuman codon optimizedPromoterAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC57-mitoLbNOX
Plasmid#74448PurposeThis plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.DepositorInsertmitoLbNOX
UseTagsFlag tag with linker and mitochondrial targeting …ExpressionMutationHuman codon optimizedPromoterAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBA-mCherry-EGFP-PIM
Plasmid#111758PurposeLow level expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 additionDepositorInsert2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP
UseTagsExpressionMammalianMutationVal24Glu, Tyr80Cys, Ala95Thr mutations in second …Promoterchicken beta-actinAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCytERM_mScarlet_N1
Plasmid#85066PurposeIn vivo visualization of the ER (can be used for colocalization studies and the OSER assay)DepositorInsertCytERM
UseTagsmScarletExpressionMammalianMutationaa1-29PromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only