We narrowed to 18,759 results for: nls
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Plasmid#202044PurposeEncodes a DNase-dead AsCpf1 fused to a 3x SV40 nuclear localization signal (NLS) and VP64 transactivation domain. 3' UTR has a MALAT1 triplex, dCas12a direct repeats (DR), and a crRNA cloning site.DepositorInsertddCas12a(E993A)-3xNLS-VP64
ExpressionMammalianAvailable sinceAug. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pL6puro-JeT-pmRevErbA-Electra2-NLS-Pest
Plasmid#240111PurposeA lentiviral (blue) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by synthtic JeT promoter.DepositorPublicationInsertmurine Nr1d1 promoter+Electra2-NLS-Pest-Reporter (Nr1d1 Mouse)
UseLentiviralMutationadded JeT PromoterAvailable sinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMDJ13 - pEXP[Peft-3 | tagRFP-T (NLS) | tbb-2 UTR]
Plasmid#159800PurposeExpression construct with ubiquitous expression to test fluorescence level on microscopeDepositorInsertpEXP[Peft-3 | tagRFP-T (NLS) | tbb-2 UTR]
ExpressionWormMutationNot applicableAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM232 - [Pmlc-1 | tagRFP-T (2x NLS)| cbr-tbb-2 UTR]
Plasmid#159898PurposeFluorescent co-injection marker to identify extrachromosomal arrays in C. elegansDepositorInsertPmlc-1 | tagRFP-T (2x NLS)| cbr-tbb-2 UTR
ExpressionWormAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
ptagBFP-TetR-NLS-VP16
Plasmid#103835PurposeTranscription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arraysDepositorAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSDest2-SpCas9-NLS-3XHA-NLS-Zif268
Plasmid#69219PurposeExpresses wild type SpCas9 fused to Zif268 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertZif268 (Egr1 Mouse)
UseCRISPRTagsNLS-3XHA-NLSExpressionMammalianAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCSD_SpCas9MT3-NLS-3xHA-NLS-SaCas9WT-2xNLS
Plasmid#107322PurposeExpresses SpCas9 (R1335K) fused to SaCas9 in mammalian cellsDepositorInsertSpCas9-SaCas9 fusion
UseCRISPRTagsNLSExpressionMammalianMutationSpCas9 (R1335K) is fused to SaCas9PromoterCMV IE93Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pW299-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-tagBFP-P2A-BlastR
Plasmid#189943PurposeLentiviral vector to co-express an spsgRNA with NLS-tagBFPDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
JAB2500
Plasmid#190379PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-BetIO::GFPint-NLS_ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_pro35S-BetIO::GFPint-NLS_ADHt
ExpressionPlantAvailable sinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWP172
Plasmid#198796PurposeCo-expression plasmid for the OLL neuronsDepositorInsertPseb-3-NLS-gp41-1-C-intein-cGAL(AD)
ExpressionWormAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWP170
Plasmid#198792PurposeCo-expression plasmid for the FLP, PVD neuronsDepositorInsertPY48G10A.6-NLS-gp41-1-C-intein-cGAL(AD)
ExpressionWormAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWP101
Plasmid#198791PurposeCo-expression plasmid for the FLP, PVD neurons or the PHC neuronsDepositorInsertPkcnl-4-NLS-cGAL(DBD)-gp41-1-N-intein
ExpressionWormAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
STEME-4
Plasmid#138140PurposeTargeted simultaneous C-to-T and A-to-G in rice protoplastsDepositorInsertwtTadA-TadA7.10-APOBEC3A-nCas9-2X(T2A-UGI-NLS)
ExpressionPlantAvailable sinceAug. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
STEME-3
Plasmid#138139PurposeTargeted simultaneous C-to-T and A-to-G in rice protoplastsDepositorInsertAPOBEC3A-wtTadA-TadA7.10-nCas9-2X(T2A-UGI-NLS)
ExpressionPlantAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1841
Plasmid#154338PurposePromoter reporterDepositorInsertSV40 NLS::mScarlet_I(dpi)::-tbb-2 3'UTR
ExpressionWormMutationSilent mutations to remove piRNA sitesAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT176_GalL_eA3A-BE3
Plasmid#145125PurposeExpressing base editor eA3A-BE3 in yeast cellsDepositorInsertA3A(N57G)-BE3
UseCRISPRExpressionYeastMutationA3A(N57G); spCas9(D10A)PromoterGalLAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT174_GalL_A3A-Y130F-BE3
Plasmid#145123PurposeExpressing base editor A3A-Y130F-BE3 in yeast cellsDepositorInsertA3A(Y130F)-BE3
UseCRISPRExpressionYeastMutationA3A(Y130F); spCas9(D10A)PromoterGalLAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT173_GalL_A3A-R128A-BE3
Plasmid#145122PurposeExpressing base editor A3A-R128A-BE3 in yeast cellsDepositorInsertA3A(R128A)-BE3
UseCRISPRExpressionYeastMutationA3A(R128A); spCas9(D10A)PromoterGalLAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT113_GalL_cCDA1-xBE3
Plasmid#145079PurposeExpressing base editor cCDA1-xBE3 in yeast cellsDepositorInsertcCDA1-xBE3
UseCRISPRExpressionYeastMutationspCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
ARB287d
Plasmid#124018PurposeEncodes pEF1a driving expression of mAzamiGreen with a spacer 3' UTR and a mScarlet normalization in a PiggyBac destination vectorDepositorInsertPB_pEF1a-NLS-mAzamiGreen-spacer_pCAG-H2B::mScarlet-rglpA
ExpressionMammalianAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
ARB287e
Plasmid#124019PurposeEncodes pEF1a driving expression of mAzamiGreen with a multicistronic spacer 3' UTR and a mScarlet normalization in a PiggyBac destination vectorDepositorInsertPB_pEF1a-NLS-mAzamiGreen-spacermulticistronic_pCAG-H2B::mScarlet-rglpA
ExpressionMammalianAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
JPF0507
Plasmid#123998PurposeEncodes pPGK1 driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vectorDepositorInsertPB_pPGK1-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
ExpressionMammalianAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
JPF0508
Plasmid#123999PurposeEncodes pCAG driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vectorDepositorInsertPB_pCAG-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
ExpressionMammalianAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
JPF0509
Plasmid#124000PurposeEncodes pPGK1 driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vectorDepositorInsertPB_pSV40-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
ExpressionMammalianAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
JPF0513
Plasmid#124004PurposeEncodes pCMV-A driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vectorDepositorInsertPB_pCMV-A-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
ExpressionMammalianAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
JPF0521
Plasmid#124012PurposeEncodes crippled pPGK1 driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vectorDepositorInsertPB_pPGK1c-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
ExpressionMammalianAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
JPF0523
Plasmid#124014PurposeEncodes crippled pSFFV driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vectorDepositorInsertPB_pSFFVc-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
ExpressionMammalianAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
ARB287a
Plasmid#124015PurposeEncodes pEF1a driving expression of mAzamiGreen with a BgpPA 3' UTR and a mScarlet normalization in a PiggyBac destination vectorDepositorInsertPB_pEF1a-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
ExpressionMammalianAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
ARB287b
Plasmid#124016PurposeEncodes pEF1a driving expression of mAzamiGreen with a SV40 3' UTR and a mScarlet normalization in a PiggyBac destination vectorDepositorInsertPB_pEF1a-NLS-mAzamiGreen-SV40pA_pCAG-H2B::mScarlet-rglpA
ExpressionMammalianAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
ARB287c
Plasmid#124017PurposeEncodes pEF1a driving expression of mAzamiGreen with a RglPA 3' UTR and a mScarlet normalization in a PiggyBac destination vectorDepositorInsertPB_pEF1a-NLS-mAzamiGreen-rglpA_pCAG-H2B::mScarlet-rglpA
ExpressionMammalianAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-nSpRY(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES395)
Plasmid#185489PurposeCMV and T7 promoter expression plasmid for human codon usage nSpRY(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFPDepositorInserthuman codon usage nSpRY(H840A)-BPNLS-P2A-eGFP
UseIn vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-eGFPExpressionMammalianMutationH840A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N13…PromoterCMV and T7Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-nSpRY(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES636)
Plasmid#185491PurposeCMV and T7 promoter expression plasmid for human codon usage nSpRY(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman codon usage nSpRY(D10A)-BPNLS-P2A-eGFP
UseIn vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-eGFPExpressionMammalianMutationD10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterCMV and T7Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pME-nls-sfGFP
Plasmid#132973Purposefull-length of nuclear localized sfGFP with stop codon in pmE Gateway vectorDepositorInsertnuclear localized super folder GFP
UseGateway vectorTags6x myc and SV40 NLSAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-NLS-dCas13-NLS-eNAT10
Plasmid#238300PurposeExpresses nuclear version of the programmable RNA acetylation system.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdPspCas13b fused to eNAT10 (NAT10 Human, Prevotella sp. P5-125)
UseCRISPRTagsSV40 BPNLS and SV40 BPNLS (C terminal of dPspCas1…ExpressionMammalianMutationH133A for catalytically inactive mutant, deletion…Available sinceJune 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN nls-BFP-FLAG-WPRE-bgh-pA (JDW 452)
Plasmid#229827PurposeA CAGGS driven nls BFP with a FLAG tag followed by a WPRE-bGH polyA for stable transcriptsDepositorInsert3xnls-TagBFP2-MCS-FLAG
ExpressionMammalianAvailable sinceMay 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-myc NES-mCE NLS-Flag
Plasmid#82468PurposeExpresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzymeDepositorAvailable sinceSept. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-ArgiNLS-mGreenLantern
Plasmid#220596PurposeConstitutive expression of a single-cell discriminating version of mGreenLantern fluorescent protein.DepositorInsertmGreenLantern
UseAAVTagsArgiNLSExpressionMammalianPromoterEF1aAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG>nls-Cas9-nls-2A-Citrine-EcoRI/NotI
Plasmid#169097PurposeCas9 and Citrine expression in transfected cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9-2A-Citrine
ExpressionMammalianAvailable sinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-NLS-3xFlag
Plasmid#132402Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralTagsflagExpressionMammalianMutationH133A; H1059AAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE-IRES-puro-NLS-dAdmCas13d-EGFP-NLS-3xFlag
Plasmid#132412Purposeoverexpression in human cellsDepositorInsertdAdmCas13b
UseLentiviralTagsflagExpressionMammalianMutationR269A; H274A; R837A; H842AAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only