We narrowed to 7,668 results for: CCH

1. BS-ZJLEU-PTEF1-HFL1-W371A-GFP

   Plasmid

   #207019

   Purpose

   Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   HFL1
   

   Tags

   GFP

   Expression

   Yeast

   Mutation

   W371A

   Promoter

   pTEF1

   Available Since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. ClhN-P1K-3VSV-Atg8-MET15

   Plasmid

   #207055

   Purpose

   Expression of 3VSV-Atg8. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   ATG8
   

   Tags

   3xVSV

   Expression

   Yeast

   Promoter

   pATG8

   Available Since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD-P19-EGFP

   Plasmid

   #196609

   Purpose

   siRNA vector expressing TMV P19 and a 720-long EGFP sequence as inverted repeat

   Depositor

   Franco Lucchini

   Article

   Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

   Insert

   p19 + EGFP inverted repeat sequence
   

   Tags

   6x His tag

   Expression

   Bacterial

   Promoter

   AraC

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAD-P19-NP

   Plasmid

   #196608

   Purpose

   siRNA vector expressing TMV P19 and a 425bp-long NP sequence as inverted repeat

   Depositor

   Franco Lucchini

   Article

   Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

   Insert

   p19 + Influenza virus NP inverted repeat
   

   Tags

   6x His tag

   Expression

   Bacterial

   Available Since

   May 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. psiCHECK-NP

   Plasmid

   #196607

   Purpose

   Dual-Luciferase Reporter vector carrying a 425bp-long Influenza virus NP sequence in forward direction

   Depositor

   Franco Lucchini

   Article

   Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

   Insert

   nucleocapsid protein (NP) gene, partial
   

   Expression

   Mammalian

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-WIPI2d (FRRG/AAAA)-TS

   Plasmid

   #190037

   Purpose

   Expression of wipi2d mutant

   Depositor

   James Hurley

   Article

   Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

   Insert

   WIPI2d (WIPI2 Human)
   

   Tags

   TEV-2xStrep tag

   Expression

   Mammalian

   Mutation

   223FRRG to AAAA

   Promoter

   CMV

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIALD2S

   Plasmid

   #185865

   Purpose

   Knocking out ALD6 and expressing anacetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP ) in Saccharomyces cerevisiae

   Depositor

   Claudia Vickers

   Article

   Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

   Insert

   ALD6 (-125, 40)> PADH1> Lr.pduP> TPDC1-ALD6 (1054, 1749)
   

   Expression

   Yeast

   Mutation

   None

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET29b-AviTag-mEGFP-dspB(E184Q W330Y)-6xHis

   Plasmid

   #176575

   Purpose

   Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

   Insert

   Dispersin B
   

   Tags

   Avitag, Hexahistidine tag, and mEGFP

   Expression

   Bacterial

   Mutation

   E184Q W330Y

   Promoter

   T7

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCSN071

   Plasmid

   #166729

   Purpose

   Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae

   Depositor

   Rene Verwaal

   Article

   Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

   Insert

   dCas12a-Mxi1-NLS
   

   Use

   CRISPR

   Tags

   Mxi1 and SV40 NLS

   Expression

   Yeast

   Mutation

   E925A

   Promoter

   S. cerevisiae TEF1

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCSN072

    Plasmid

    #166730

    Purpose

    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae

    Depositor

    Rene Verwaal

    Article

    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

    Insert

    dCas12a-NLS-Mxi1-NLS
    

    Use

    CRISPR

    Tags

    Mxi1 and SV40 NLS

    Expression

    Yeast

    Mutation

    E925A

    Promoter

    S. cerevisiae TEF1

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Dom34 E23K/F218A

    Plasmid

    #19106

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Protein DOM34 (DOM34 Budding Yeast)
    

    Tags

    C tag

    Expression

    Bacterial

    Mutation

    E23K/F218A

    Available Since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pLIB-HIS-TEV-Cyclin-B

    Plasmid

    #177012

    Purpose

    Generate baculovirus for insect cell expression of Cyclin-B with N-terminal Polyhistidine-TEV

    Depositor

    Andrea Musacchio

    Article

    Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

    Insert

    Cyclin B1 (CCNB1 Human)
    

    Tags

    Polyhistidine-TEV

    Expression

    Insect

    Mutation

    codon-optimised for insect cell expression

    Available Since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. HBM-Luc

    Plasmid

    #35155

    Depositor

    Linda Penn

    Article

    Facchini et al Mol Cell Biol. 1997 Jan;17(1):100-14.

    Insert

    Myc promoter (MYC Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Contains 2.5 kb of the c-myc minimal promoter

    Promoter

    c-myc

    Available Since

    Oct. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-4T1_ATG4B_BamHI_NotI

    Plasmid

    #190862

    Purpose

    Plasmid for the expression and purification of of ATG4B. Internal reference: SMC1392

    Depositor

    Sascha Martens

    Article

    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    ATG4B (ATG4B Human)
    

    Tags

    GST, Thrombin Cleavage site

    Expression

    Bacterial

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUDE1093

    Plasmid

    #204227

    Purpose

    Episomal yeast plasmid expressing the Ercas12a

    Depositor

    Jean-Marc Daran

    Article

    Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d

    Insert

    Eubacterium rectale cas12a (Ercas12a)
    

    Expression

    Yeast

    Mutation

    codon optimized for S. cerevisiae

    Promoter

    Saccharomyces cerevisiae PGK1

    Available Since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCW-OVOL2-HA

    Plasmid

    #184737

    Purpose

    Doxycycline-inducible lentiviral expression of OVOL2

    Depositor

    Gloria Manzotti, Alessia Ciarrocchi

    Article

    Gugnoni et al J Exp Clin Cancer Res. 2022 Mar 25;41(1)

    Insert

    ovo like zinc finger 2 (OVOL2 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    Tet ON

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCSN061

    Plasmid

    #101725

    Purpose

    Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.

    Depositor

    Rene Verwaal

    Article

    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

    Inserts

    S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
    KanMX marker expression cassette.
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Promoter

    Heterologous TEF1 promoter from A. gossypii. and …

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCLX-MIFE-hM3Dq-PGK-mCherry

    Plasmid

    #114294

    Purpose

    Mifepristone-inducible expression of hM3Dq protein

    Depositor

    Patrick Salmon

    Article

    Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

    Insert

    hM3Dq
    

    Use

    Lentiviral

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. BS-ZJLEU-PTEF1-HFL1-W371A I375A D384A Y387A-GFP

    Plasmid

    #207025

    Purpose

    Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    W371A I375A D384A Y387A

    Promoter

    pTEF1

    Available Since

    June 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. PCUP1-GFP-Pho8-406

    Plasmid

    #207006

    Purpose

    Integration of GFP-Pho8 as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Marker for yeast vacuolar membrane.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    PHO8
    

    Tags

    GFP

    Expression

    Yeast

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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