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scAAV-TTR-mFgf15

Plasmid

#190594

Purpose

AAV construct containing Fgf15 cds under control of TTR promoter

Depositor

Roel Nusse

Article

Sarkar et al Elife. 2023 Jan 31;12:e82311. doi: 10.75

Insert

Fgf15 (Fgf15 Mouse)

Use

AAV

Expression

Mammalian

Promoter

Ttr

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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HOXB5 (human) FLAG-MSCV

Plasmid

#8532

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB5 (HOXB5 Human)

Use

Retroviral

Tags

FLAG and IRES-GFP

Expression

Mammalian

Mutation

contains improved ribosomal binding site and FLAG…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pOPINK-ALG13 (OTU, aa 216-353)

Plasmid

#61414

Purpose

Expresses human ALG13 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

ALG13 (Putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13) (ALG13 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-215 and aa 354-1137.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mPC1_4119-3HA

Plasmid

#108395

Purpose

Express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

Pkd1 (Pkd1 Mouse)

Tags

3HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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HOXB6 (human) EGFP-fusion

Plasmid

#8535

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB6 (HOXB6 Human)

Tags

EGFP

Expression

Mammalian

Mutation

contains eGFP fused to N-terminal of HOXB6 at AA…

Promoter

CMV

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-mPC1_4077-3HA

Plasmid

#108392

Purpose

Express cytoplasmic tail mouse PC1 from amino acid 4077 to 4293

Depositor

Gregory Germino

Article

Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

Insert

Pkd1 (Pkd1 Mouse)

Tags

3HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE-MCS(extended)-Puro (empty vector)

Plasmid

#172603

Purpose

Empty retroviral vector for the constitutive, near-physiological expression of a gene of interest and a puromycin resistance marker in mammalian cells.

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Mutation

The multiple cloning site (MCS) has been edited/e…

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 KBTBD4 WT

Plasmid

#184627

Purpose

Mammalian expression vector with N-terminal 3xFlag for KBTBD4 WT expression in mammalian cells

Depositor

Vincenzo D'Angiolella

Article

Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

Insert

N-terminal 3xFlag tagged KBTBD4 WT (KBTBD4 Human)

Expression

Mammalian

Mutation

no mutation

Promoter

CMV

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-ING2(pHD)

Plasmid

#21589

Depositor

Junying Yuan

Article

Gozani et al Cell. 2003 Jul 11. 114(1):99-111.

Insert

GFP-ING2(pHD) (Ing2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

contains Ing2 plant homeodomain (PHD) amino acids…

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only
pAAV-hsyn-Jaws-KGC-GFP-ER2

Plasmid

#65014

Purpose

AAV-mediated expression of Jaws under the human synapsin promoter

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Has Service

AAV Retrograde, AAV5, and AAV8

Insert

Jaws-KGC-GFP-ER2

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Mutation

K200R W214F

Promoter

human synapsin

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 c-SRC (WT)

Plasmid

#42202

Depositor

Robert Lefkowitz

Article

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

c-SRC (SRC Human)

Expression

Mammalian

Mutation

L176F mutation compared to reference sequence NM_…

Promoter

CMV

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 SIRT1-FL

Plasmid

#105670

Purpose

Mammalian expression construct of mouse SIRT1 full length isoform

Depositor

Ullas Kolthur-Seetharam

Article

Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

Insert

SIRT1 (Sirt1 Mouse)

Expression

Mammalian

Mutation

Synonymous base changes not affecting the protein…

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-Syn-Jaws-KGC-tdTomato-ER2

Plasmid

#153535

Purpose

AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the Syn promoter.

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-KGC-tdTomato-ER2

Use

AAV

Tags

ER2, KGC, and tdTomato

Expression

Mammalian

Mutation

K200R W214F

Promoter

Syn

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-CaMKII-Jaws-KGC-tdTomato-ER2

Plasmid

#153534

Purpose

AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CaMKII promoter.

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws-KGC-tdTomato-ER2

Use

AAV

Tags

ER2, KGC, and tdTomato

Expression

Mammalian

Mutation

K200R W214F

Promoter

CaMKII

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe-Puro-Flag-IKBKE

Plasmid

#15292

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

IKBKE (IKBKE Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
pBabe-Puro-Myr-Flag-IKBKE

Plasmid

#15293

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

IKBKE (IKBKE Human)

Use

Retroviral

Tags

Myr-Flag

Expression

Mammalian

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
pBabe-Puro-cRAF-W22

Plasmid

#15270

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CRAF (RAF1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

C-terminal deletion

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
pGL3-noSV40-humanEC1.45_min1-2

Plasmid

#173952

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe-Neo-Flag-IKBKE

Plasmid

#15265

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

IKBKE (IKBKE Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
p(HA)HIF1alpha(401delta603)R27G

Plasmid

#52215

Purpose

mammalian expression of HA tagged HIF1alpha with a deletion of the N-terminal activation domain and R27G mutation

Depositor

Eric Huang

Article

Koshiji et al EMBO J. 2004 May 5;23(9):1949-56. Epub 2

Insert

HIF1alpha (401delta603) R27G (HIF1A Human)

Tags

HA

Expression

Mammalian

Mutation

aa 401-603 deleted, removes the oxygen-dependent …

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only