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Plasmid#232319PurposepZP05 was constructed by cloning the oriFn-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, facilitating replication in Fusobacterium nucleatumDepositorInsertOri-repA
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-AVO1
Plasmid#232889PurposePlasmid expressing Cas9 and gRNA AATGATGACGACCATACCAG which targets the AVO1 gene.DepositorInsertAVO1 gRNA (AVO1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-YFR054C
Plasmid#232900PurposePlasmid expressing Cas9 and gRNA GCTCAAAGAAACAATTAGAG which targets the YFR054C gene.DepositorInsertYFR054C gRNA (YFR054C Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-ZIM17
Plasmid#232901PurposePlasmid expressing Cas9 and gRNA AAATGTCTCACTTTGCAGTG which targets the ZIM17 gene.DepositorInsertZIM17 gRNA (ZIM17 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-SRP14
Plasmid#232896PurposePlasmid expressing Cas9 and gRNA GAAAACAAAATGCTCAACAG which targets the SRP14 gene.DepositorInsertSRP14 gRNA (SRP14 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-TLG1
Plasmid#232897PurposePlasmid expressing Cas9 and gRNA GATGAAAACGAAGACGTGAG which targets the TLG1 gene.DepositorInsertTLG1 gRNA (TLG1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-VHT1
Plasmid#232898PurposePlasmid expressing Cas9 and gRNA TGCGGCCACACTGAAATACG which targets the VHT1 gene.DepositorInsertVHT1 gRNA (VHT1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-ADE13
Plasmid#232887PurposePlasmid expressing Cas9 and gRNA GTAGCAGCAAAAGAAGACAA which targets the ADE13 gene.DepositorInsertADE13 gRNA (ADE13 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-LAS17
Plasmid#232892PurposePlasmid expressing Cas9 and gRNA AATACCCTGAATTCTGCCGG which targets the LAS17 gene.DepositorInsertLAS17 gRNA (LAS17 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML104-LEU2-2
Plasmid#232883PurposePlasmid expressing Cas9 and gRNA GGTAGTGTTAGACCTGAACA which targets the LEU2 gene.DepositorInsertLEU2 gRNA (LEU2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS313-rad53-5004
Plasmid#232902PurposePlasmid carrying the rad53-5004 allele with its native promoter and terminator.DepositorInsertRAD53 (RAD53 Budding Yeast)
UseTagsExpressionYeastMutationT600C, A680G, A1094T, C1615T, C2228APromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-INO80
Plasmid#232890PurposePlasmid expressing Cas9 and gRNA GGAAGTAGAATCATTCGTAG which targets the INO80 gene.DepositorInsertINO80 gRNA (INO80 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415 GPDpro-RNQ1-CFP
Plasmid#224887PurposeLow copy plasmid for constitutive yeast expression of RNQ1 tagged with CFP using copper. This is construct can be used as an IPOD marker (Kaganovich et al., 2008).DepositorInsertRNQ1 (RNQ1 Budding Yeast)
UseTagsCFPExpressionYeastMutationPromoterGPDAvailable sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
INT_Cargo_T7_gutB
Plasmid#212139PurposeCargo plasmid for integrating PT7 expression cassette of xylitol dehydrogenase in in genome.Plasmid can be removed by incubating cells at 37 C.DepositorInsertxylitol dehydrogenase
UseTagsExpressionBacterialMutationPromoterPT7Available sinceMarch 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCT03
Plasmid#212150PurposePlasmid expressing alanine dehydrogenase under inducible promoter PT7 control. The enzyme is thermophilic and works best at 50 C.DepositorInsertalanine dehydrogenase
UseTags6x HisExpressionBacterialMutationPromoterPT7Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
SynCAM4/pCDNA3
Plasmid#197328PurposePlasmid expressing an antigen targeting NECL4/SynCAM4DepositorInsertSynCAM4 (Cadm4 Mouse)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-mCherry-WPRE-UbC-Emerald
Plasmid#211797PurposeLentiviral vector plasmid expressing mCherry under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsmCherry
Emerald
UseLentiviralTagsExpressionMammalianMutationPromoterSFFV and UbCAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
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Kv1.1/RBG4
Plasmid#196943PurposePlasmid expressing an antigen targeting the Kv1.1 potassium channel subunitDepositorInsertKv1.1 (Kcna1 Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
KChIP2/RBG4; 9QM3/RBG4
Plasmid#196944PurposePlasmid expressing an antigen targeting the KChIP2b potassium channel subunitDepositorInsertKChIP2 (Kcnip2 Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only