We narrowed to 9,967 results for: EPO

1. YFP-Rpp20-C1

   Plasmid

   #134541

   Purpose

   Expresses YFP-Rpp20 fusion protein

   Depositor

   Susan Janicki

   Article

   Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

   Insert

   Rpp20
   

   Tags

   YFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-FNLSHiFi-P2A-Puro

   Plasmid

   #136902

   Purpose

   Lentivirus for constitutive expression of FNLSHiFi in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   FNLSHiFi
   

   Use

   Lentiviral

   Tags

   FLAG-NLS and NLS

   Mutation

   R691A

   Promoter

   EF1s

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTK_Citrine_Nanotag_5_neg_Control

   Plasmid

   #130558

   Purpose

   Enhancer cloning nanotag reporter vector

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

   Insert

   negative oligo sequence
   

   Use

   Enhancer cloning nanotag reporter vector

   Promoter

   thymidine kinase minimal promoter

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. p416GAL1-Zipp.-L-IFP-F[1] (Yeast)

   Plasmid

   #52895

   Purpose

   Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[1] by the linker (GGGGS) X 2. Zipper-L-IFP-F[1] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.

   Depositor

   Stephen Michnick

   Article

   Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

   Insert

   Zipp.-L-IFP-F[1]
   

   Tags

   L-IFP-F[1]

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

5. Myog'-2A-mCherryNLS-PGK-Purodtk

   Plasmid

   #69547

   Purpose

   Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

   Depositor

   Charles Gersbach

   Inserts

   2A-mCherry-NLS
   Myogenin 5' homology arm (Myog Mouse)
   Myogenin 3' homology arm (Myog Mouse)
   Puro-delta-tk
   

   Use

   Mouse Targeting

   Tags

   2x nuclear localization signal and PVT1-2A "…

   Mutation

   Silent V205V mutation in myogenin gene to prevent…

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. A3F-Cas9n-UGI

   Plasmid

   #119138

   Purpose

   Base editor made from APOBEC3F

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   APOBEC3F-Cas9 nickase-UGI
   

   Use

   CRISPR

   Mutation

   None

   Promoter

   CMV

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. A3C-Cas9n-UGI

   Plasmid

   #119136

   Purpose

   Base editor made from APOBEC3C

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   APOBEC3C-Cas9 nickase-UGI
   

   Use

   CRISPR

   Mutation

   None

   Promoter

   CMV

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOIMars1CyN

   Plasmid

   #75221

   Purpose

   Mammalian cell surface expression of fluorogen activating protein Mars1Cy

   Depositor

   Alan Waggoner

   Article

   Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

   Insert

   ss-HA-Mars1Cy-CD80(237-306)
   

   Tags

   HA epitope

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTK-EdnrbE2-Citrine

   Plasmid

   #127770

   Purpose

   Enhancer reporter construct containing the chicken EdnrB enhancer, E2 driving Citrine activity

   Depositor

   Tatjana Sauka-Spengler

   Article

   Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

   Insert

   ednrbE2
   

   Use

   Enhancer reporter construct

   Available Since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKI-cjVASA-tdTomato

    Plasmid

    #160872

    Purpose

    Marmoset Targeting

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Stem Cell Res. 2021 Mar 24;53:102308. do

    Insert

    tdTomato
    

    Use

    Marmoset targeting

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLI_C-tag TNF linker mCherry

    Plasmid

    #171181

    Purpose

    inducible expression of mCherry fused to C-tag TNF

    Depositor

    Veit Hornung

    Article

    Pinci et al J Biol Chem. 2020 Dec 25;295(52):18065-1

    Insert

    C-tag TNF linker mCherry
    

    Use

    Lentiviral

    Tags

    C-tag (internal) and mCherry

    Expression

    Mammalian

    Promoter

    tight TRE promoter

    Available Since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLI_BFP_C-tag TNF

    Plasmid

    #171187

    Purpose

    inducible expression of C-tag TNF

    Depositor

    Veit Hornung

    Article

    Pinci et al J Biol Chem. 2020 Dec 25;295(52):18065-1

    Insert

    C-tag TNF
    

    Use

    Lentiviral

    Tags

    C-tag (internal)

    Expression

    Mammalian

    Mutation

    ADAM cleavage site of TNF (AA73-76) was exchanged…

    Promoter

    tight TRE promoter

    Available Since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pILGFP3

    Plasmid

    #65451

    Purpose

    pILGFP3 was designed to test the promoter activity using the yeast enhanced green fluorescent protein (yEGFP) as reporter.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Type

    Empty backbone

    Tags

    yEGFP reporter

    Expression

    Yeast

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pITGFP3

    Plasmid

    #65452

    Purpose

    pITGFP3 was designed to test the promoter activity using the destabilized yeast enhanced green fluorescent protein (yEGFP-CLN2PEST) as reporter.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Type

    Empty backbone

    Tags

    yEGFP-CLN2PEST reporter

    Expression

    Yeast

    Available Since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

15. A3G-Cas9n-UGI

    Plasmid

    #119139

    Purpose

    Base editor made from APOBEC3G

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    APOBEC3G-Cas9 nickase-UGI
    

    Use

    CRISPR

    Mutation

    None

    Promoter

    CMV

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAP1638

    Plasmid

    #137089

    Purpose

    Plasmid for PCR-generated donor DNA for rapid tagging of proteins with 4*(GFP11)

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

    Insert

    4*(GFP11)
    

    Use

    Pcr template for donor dna

    Expression

    Bacterial

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAP1836

    Plasmid

    #137087

    Purpose

    Plasmid for PCR-generated donor DNA for rapid tagging of proteins with mCherry

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

    Insert

    mCherry
    

    Use

    Pcr template for donor dna

    Expression

    Bacterial

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3_AKAR5Δ

    Plasmid

    #119912

    Purpose

    Mammalian expression of the AKAR5Δ sensor with N-terminal His tag for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

    Depositor

    Haining Zhong

    Article

    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

    Insert

    AKAR5Δ
    

    Tags

    6xHis

    Expression

    Mammalian

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. p413GAL1-Zipp.-L-IFP-F[2] (Yeast)

    Plasmid

    #52893

    Purpose

    Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[2] by the linker (GGGGS) X 2. Zipper-L-IFP-F[2] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    Zipp.-L-IFP-F[2]
    

    Tags

    L-IFP-F[2]

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pUAS-kkv-mos::NG

    Plasmid

    #138958

    Purpose

    Used to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila where the active site is converted to the mosquito sequence. Expression by using Gal4

    Depositor

    Paul Adler

    Article

    Adler et al bioRxiv 2020

    Insert

    kkv (NEWENTRY Fly)
    

    Use

    Uas transgene vector

    Tags

    Neon Green fused to C terminus of Kkv

    Expression

    Insect

    Mutation

    8 bp that are in the region that encodes the cata…

    Promoter

    UAS

    Available Since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pNC1137

    Plasmid

    #41563

    Depositor

    Beverly Errede

    Article

    Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

    Insert

    Ura3-FUS1-UBIMdkGFP*-SpHis5-Tim9
    

    Mutation

    GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

    Available Since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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22. VV221: sdChR(C138S)-TS-eGFP-ER in fck

    Plasmid

    #58513

    Purpose

    Expresses sdChR with the C138S (step function opsin) mutation fused to eGFP under the a-CamKII promoter

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    sdChR(C138S)-TS-eGFP-ER
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    changed Cysteine 138 to Serine

    Promoter

    a-CamKII

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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23. pP2AeGFP-Mars1Cy

    Plasmid

    #75223

    Purpose

    Mammalian intracellular expression of fluorogen activating protein Mars1Cy and eGFP

    Depositor

    Alan Waggoner

    Article

    Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

    Insert

    EGFP-p2a-Mars1Cy
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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24. pRK5_tAKARγ

    Plasmid

    #119915

    Purpose

    Mammalian expression of the tAKARγ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

    Depositor

    Haining Zhong

    Article

    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

    Insert

    tAKARγ
    

    Expression

    Mammalian

    Available Since

    April 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. P199

    Plasmid

    #139717

    Purpose

    Module plasmid, individual component of Fungal Bioluminescent Pathway, Luz, Luz promoter is circadian controlled

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pMOD_D_pAtELF4:NnLuz:trbcSE9
    

    Use

    Luciferase

    Expression

    Plant

    Promoter

    AtELF4

    Available Since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. pBS-PtH

    Plasmid

    #133391

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (donor repair template for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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27. BII-C3HTK

    Plasmid

    #133388

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pKM149

    Plasmid

    #134178

    Purpose

    Bacterial expression of his-CENP-C 510-end

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Cell. 2014 Jul 17;158(2):397-411. doi: 1

    Insert

    his-CENP-C 510-end (CENPC Human)
    

    Expression

    Bacterial

    Mutation

    aa 510-end

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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29. pSB1A2QS

    Plasmid

    #138268

    Purpose

    MINPA Gene circuit expression in E. coli. GFP serves as a reporter for Plux/tet promoter activity. mCherry serves as reporter for Para/lac promoter activity

    Depositor

    Xiao Wang

    Article

    Wu et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

    Inserts

    AraC-TetR operon
    LuxR-LacI operon
    mCherry
    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Para/lac and Plux/tet

    Available Since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAP1837

    Plasmid

    #137086

    Purpose

    Plasmid for PCR-generated donor DNA for rapid tagging of proteins with mCherry

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

    Insert

    mCherry
    

    Use

    Pcr template for donor dna

    Expression

    Bacterial

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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