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pcDNA5/FRT/TO-Intron-CterFlag-MePCE

Plasmid

#113549

Purpose

Mammalian expression plasmid that can be used with the Flp-In T-REx system.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Tags

Flag

Expression

Mammalian

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF16_P2A_Hygro_Barcode

Plasmid

#120502

Purpose

Barcoded lentiviral vector to express KLF16 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF16 (KLF16 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAC149-pCR8-dCas9VP160

Plasmid

#48221

Purpose

dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP160 activation domain

Use

CRISPR; Gateway donor

Tags

HA Tag and VP160

Mutation

D10A;H840A nuclease-deficient

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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px330-CD4

Plasmid

#136938

Purpose

NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human CD4; induce CD4+ deletion rearrangement by pairing w/ px330-GAPDH

Depositor

Jeremy Stark

Article

Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

Insert

sgRNA targeting CD4 (CD4 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shOrai1

Plasmid

#89933

Purpose

Knockdown human Orai1 expression

Depositor

Shengyu Yang

Article

Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

Insert

Orai1 shRNA (ORAI1 Human)

Use

Retroviral

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pLNCX2-STIM1

Plasmid

#89817

Purpose

Express HUMAN STIM1

Depositor

Shengyu Yang

Article

Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

Insert

STIM1 (STIM1 Human)

Use

Retroviral

Tags

NO TAG

Available Since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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AllCB shRNA-1

Plasmid

#160065

Purpose

Knock Down all Collybistin isoforms

Depositor

Angel de Blas

Article

George et al J Neurochem. 2021 May;157(4):1032-1051.

Insert

ARHGEF9 (Arhgef9 Rat)

Use

RNAi

Promoter

mouse U6 promoter

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pEE060

Plasmid

#176817

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

611

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE050

Plasmid

#176807

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

601

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-BE3-FNLS(RA)

Plasmid

#112671

Purpose

CMV expression vector for BE3-FNLS construct (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3-FNLS

Tags

3x FLAG

Expression

Mammalian

Mutation

NLS sequence at the N-terminus and D10A

Promoter

CMV

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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mCh-LgBiT-2A-EGFP-Vin-PILATeS-CT

Plasmid

#216763

Purpose

PiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS-CT (700 pM) negative control in mammalian cells. Requires transposase

Depositor

Alex Dunn

Article

Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

Insert

mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS-CT

Tags

EGFP and mCherry

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pET-KIX (586-672)

Plasmid

#99336

Purpose

Bacterial expression of mouse CBP KIX domain

Depositor

Peter Wright

Article

Radhakrishnan et al J Mol Biol. 1999 Apr 16;287(5):859-65.

Insert

CBP KIX (586-672) (Crebbp Mouse)

Expression

Bacterial

Promoter

T7

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pTwist-WIV1-CoV

Plasmid

#164438

Purpose

Encodes WIV1-CoV Spike Protein for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

WIV1-CoV Spike

Expression

Mammalian

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shSTIM1

Plasmid

#89816

Purpose

Knockdown human STIM1 expression

Depositor

Shengyu Yang

Article

Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

Insert

STIM1 shRNA (STIM1 Human)

Use

Retroviral

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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EF1a_MESP1_P2A_Hygro_Barcode

Plasmid

#120459

Purpose

Barcoded lentiviral vector to express MESP1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MESP1 (MESP1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2-FLAG-hEcto/Tif1g-CAmut

Plasmid

#20903

Depositor

Stefano Piccolo

Article

Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

Insert

Ectodermin (TRIM33 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Point mutagenesis of C125 and C128 to Alanine (RI…

Available Since

May 15, 2009

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-ChRmine-eYFP-Kv2.1-WPRE

Plasmid

#130993

Purpose

AAV vector to drive the expression of soma-targeted ChRmine-eYFP under the control of human synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

EYFP-Kv2.1

Promoter

hSyn

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 myc-NL-GW

Plasmid

#113446

Purpose

NanoLuc Gateway shuttle vector for N-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Luciferase; Gateway shuttle vector

Tags

cmyc-NanoLuc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-E6AP Hect

Plasmid

#165102

Purpose

bacterial expression of a GST fusion of Human E6AP Hect domain

Depositor

Arthur Haas

Article

Ronchi et al Methods Mol Biol. 2012;832:197-218. doi:

Insert

E6AP Hect (UBE3A Human)

Tags

GST from plasmid.

Expression

Bacterial

Mutation

E6AP Hect domain Amino acids 495-738 (5'QLNP…

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 GW-NL-myc

Plasmid

#113447

Purpose

NanoLuc Gateway shuttle vector for C-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Luciferase; Gateway shuttle vector

Tags

NanoLuc-cmyc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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