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GFP-RhoB

Plasmid

#23225

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

RhoB (Rhob Rat)

Tags

GFP

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3-6XHis-human SUMO 2

Plasmid

#133771

Purpose

express 6Xhis- SUMO 2 protein

Depositor

Martine Roussel

Article

Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

SUMO 2 (SUMO2 Human)

Tags

6XHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-iRFP670

Plasmid

#45453

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP670

Expression

Bacterial

Mutation

T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

Promoter

araBAD

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pZW16 (CK2alpha')

Plasmid

#27087

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha' (CSNK2A2 Human)

Tags

HA

Expression

Mammalian

Available Since

June 21, 2012

Availability

Academic Institutions and Nonprofits only

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pDONR223-FLT1

Plasmid

#23912

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FLT1 (FLT1 Human)

Use

Gateway Cloning

Mutation

S755P

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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PKC delta CAT

Plasmid

#16388

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC delta (Prkcd Mouse)

Tags

HA

Expression

Mammalian

Mutation

CAT: catalytic domain, aa 334-674

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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EGFP-Tau(WT)

Plasmid

#187023

Purpose

Expresses EGFP tagged human 2N4R tau (WT) in mammalian cells with CMV promoter

Depositor

George Bloom

Article

Rajbanshi et al Alzheimers Dement. 2023 Jan 12. doi: 10.

Insert

MAPT (MAPT Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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PCV-Cas9

Plasmid

#123643

Purpose

Expresses Cas9 with an N-terminal HUH-tag called PCV2

Depositor

Wendy Gordon

Article

Aird et al Commun Biol. 2018 May 31;1:54. doi: 10.1

Inserts

PCV2 (ORF1 )
Cas9

Tags

(EAAAK)8 linker and His6 + SUMO

Expression

Bacterial

Mutation

2-116

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pB-CAGGS-dCas9-KRAB

Plasmid

#110822

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB

Depositor

Alejandro Chavez, George Church

Article

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9-KRAB

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CAGGS

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-SENP8

Plasmid

#18066

Depositor

Edward Yeh

Article

Gao et al Nat Cell Biol. 2006 Oct . 8(10):1171-7.

Insert

SENP8 (SENP8 Human)

Tags

FLAG

Expression

Mammalian

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pCL-MFG-BRCA1

Plasmid

#12341

Depositor

Inder Verma

Article

Ruffner et al Proc Natl Acad Sci U S A. 1997 Jul 8. 94

Insert

BRCA1 (BRCA1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pLJC5-3XMyc-EGFP-PEX26

Plasmid

#139059

Purpose

Myc-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.

Depositor

David Sabatini

Article

Ray et al iScience. 2020 May 22;23(5):101109. doi:

Insert

3XMyc-EGFP-PEX26

Use

Lentiviral

Tags

Myc

Promoter

UBC

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pLNCX2-Scrib

Plasmid

#24600

Depositor

Senthil Muthuswamy

Article

Zhan et al Cell. 2008 Nov 28. 135(5):865-78.

Insert

Scribble (SCRIB Human)

Expression

Mammalian

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV4-3HA

Plasmid

#24165

Depositor

Warner Greene

Article

Sun et al Mol Cell Biol. 1996 Mar . 16(3):1058-65.

Type

Empty backbone

Tags

3xHA

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
pMXs-IP-EGFP-ULK1

Plasmid

#38193

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn FLExFRT mGFP-2A-Synaptophysin-mRuby

Plasmid

#71761

Purpose

mGFP and Synaptophysin-mRuby expression from Flp-expressing neurons

Depositor

Liqun Luo

Article

Beier et al Cell. 2015 Jul 30;162(3):622-34. doi: 10

Inserts

mGFP
Synaptophysin

Use

AAV

Tags

Palmitoylation signal and mRuby

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ150

Plasmid

#69301

Purpose

Gateway entry vector with pcoCas9 (Plant codon optimized)

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

pcoCas9 (plant codon-optimized)

Use

CRISPR

Tags

3XFLAG

Mutation

NLS fusion

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pET-His6-ERK2-MEK1_R4F_coexpression

Plasmid

#39212

Depositor

Melanie Cobb

Article

Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

Inserts

Mek1 R4F (MAP2K1 Human)
Erk2 (Mapk1 Rat)

Tags

His6 and RBS

Expression

Bacterial

Mutation

MEK1(R4F) = S218E, S222D and deletion of AA32-51

Promoter

T7 and T7 via RBS

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1his p66shc (corrected)

Plasmid

#32574

Depositor

Toren Finkel

Article

Nemoto et al Science. 2002 Mar 29. 295(5564):2450-2.

Insert

p66shc (SHC1 Human)

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pCDHblast MCSNard OST-LMNAd50

Plasmid

#22662

Depositor

Tom Misteli

Article

Pegoraro et al Nat Cell Biol. 2009 Oct . 11(10):1261-7.

Insert

LaminAd50 (=Progerin)

Use

Lentiviral

Tags

Onestrep Tag

Expression

Mammalian

Mutation

50AA deletion creating Progerin instead of mature…

Available Since

Dec. 29, 2009

Availability

Academic Institutions and Nonprofits only

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