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pCX460

Plasmid

#229215

Purpose

CRISPR array plasmid for MAP1B (Spacers 25-48)

Depositor

Jennifer Doudna

Article

Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

Insert

CRISPR array plasmid for MAP1B (Spacers 25-48)

Expression

Mammalian

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pICSL60004

Plasmid

#117519

Purpose

rbcS-E9 terminator Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GCTT:E9t:CGCT:BsaI

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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mVenus-N1

Plasmid

#54640

Purpose

Localization: N1 Cloning Vector, Excitation: 515, Emission: 528

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

Type

Empty backbone

Tags

mVenus

Expression

Mammalian

Available Since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pAVEC0_N-mNeonGreen

Plasmid

#231256

Purpose

For expressing fusion proteins with N terminally tagged mNeonGreen in plants

Depositor

Jacob Brunkard

Article

Greenhalgh et al Plant Biotechnol J. 2025 Jun 26. doi: 10

Type

Empty backbone

Tags

mNeonGreen

Expression

Plant

Mutation

N/A

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR.hU6hH1

Plasmid

#32686

Purpose

dual shRNA expression cassette entry vector

Depositor

Ming-Sound Tsao

Article

Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

Type

Empty backbone

Use

RNAi

Expression

Mammalian

Promoter

hU6hH1

Available Since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pBI/Rabaptin-5

Plasmid

#171851

Purpose

inducible expression of Rabaptin-5 in mammalian cells via co-transfection with pTet-Off.

Depositor

Guangpu Li

Article

Zhu et al PLoS One. 2010 Feb 16;5(2):e9226. doi: 1

Insert

Rabaptin-5 (human)

Expression

Mammalian

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pKmK.P2

Plasmid

#125035

Purpose

Storage plasmid for K.marxianus promoter; type 2 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

PDC1 promoter

Use

Synthetic Biology

Expression

Yeast

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pKmK.T4

Plasmid

#125056

Purpose

Storage plasmid for K.marxianus terminator; type 4 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

PDC1 terminator

Use

Synthetic Biology

Expression

Yeast

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pJOE_pvdJ

Plasmid

#110142

Purpose

Suicide plasmid with repair template for knockout in P. putida

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Type

Empty backbone

Expression

Bacterial

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pTR47m4-GFP

Plasmid

#102436

Purpose

Formaldehyde biosensor plasmid with sGFP reporter and modified frm promoter

Depositor

Gregory Stephanopoulos

Article

Woolston et al Biotechnol Bioeng. 2017 Sep 16. doi: 10.

Inserts

Formaldehyde repressor protein (frmR)
Superfolder GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pKmK.P7

Plasmid

#125040

Purpose

Storage plasmid for K.marxianus promoter; type 2 part

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

TEF1 promoter

Use

Synthetic Biology

Expression

Yeast

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pTK222

Plasmid

#13532

Depositor

Wolf Frommer

Article

Kaper et al PLoS Biol. 2007 Oct;5(10):e257.

Insert

FLIPW-CTYT

Tags

See map

Expression

Mammalian

Available Since

July 27, 2007

Availability

Academic Institutions and Nonprofits only

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pLKO.1C

Plasmid

#139470

Purpose

Replacing the RSV promoter of pLKO.1 with the CMV promoter improves lentiviral production with third-generation viral packaging system.

Depositor

David Cobrinik

Article

Lee et al Biotechniques. 2020 Jun;68(6):349-352. d

Insert

stuffer

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

CMV

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pColE1_70a_deGFP_AmpR

Plasmid

#119161

Purpose

Expresses deGFP from a strong sigma 70 promoter

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

deGFP

Use

Crispr

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTHSSe_49

Plasmid

#109247

Purpose

pSC101 var7 BbsI cloning vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23105

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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A1-20B1(P004)

Plasmid

#184056

Purpose

This plasmid carries Yn situ preamplifier A1-20B1 sequence and is used for nickase based synthesis.

Depositor

C. Ron Yu

Article

Wu et al Cell Rep Methods. 2022 Apr 18;2(4):10020

Insert

A1-20B1 preamplifier

Use

Synthetic Biology

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDASH-DIS-α2

Plasmid

#242520

Purpose

Donor I vector in DASH and alpha2 vector in GoldenBraid/DASH, with SacB counter-selection, with attBTT and attBCC-FRT

Depositor

Jose Alonso, Anna Stepanova

Article

Zhao et al Plant Biotechnol J. 2025 Jun 10. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA1

Plasmid

#75388

Purpose

sgRNA1

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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BCJJ163

Plasmid

#117512

Purpose

AtRPS5a promoter Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GGAG:pRPS5a:AATG:BsaI

Expression

Plant

Promoter

AtRPS5a

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pTHSSe_42

Plasmid

#109240

Purpose

pSC101 BbsI cloning vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23105

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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