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pOTTC469 - pAAV EF1a DIO eNpHR3.0-iRFP

Plasmid

#47631

Purpose

An AAV vector that expresses engineered halorhodopsin 3.0 (fused to iRFP) in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

Depositor

Brandon Harvey

Publication

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

eNpHR3.0

Use

AAV and Cre/Lox

Tags

iRFP

Expression

Mammalian

Promoter

EF1a

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pCAGG>nls-hCas9-nls

Plasmid

#99138

Purpose

Expresses Cas9 via the chicken beta actin promoter for electroporation in chicken embryos

Depositor

Marianne Bronner

Publication

Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

humanized Cas9

Use

CRISPR

Promoter

Chicken beta actin (CAG)

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-EF1a-DIO-PSD95-EGFP-WPRE

Plasmid

#133785

Purpose

Cre-dependent EGFP Fluorescent reporter for overexpressed postsynaptic marker PSD95 in mammalian cell

Depositor

Michael Stryker

Publication

Sun et al Proc Natl Acad Sci U S A. 2019 Oct 22;11

Insert

PSD95-EGFP (Dlg4 Rat)

Use

AAV and Cre/Lox

Tags

EGFP

Expression

Mammalian

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMSP12::Kaede

Plasmid

#30172

Depositor

Lalita Ramakrishnan

Publication

Davis et al Cell. 2009 Jan 9. 136(1):37-49.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Mycobacterium Strong Promoter (MSP)

Use

Mycobacteria expression

Tags

Kaede

Available since

June 3, 2011

Availability

Academic Institutions and Nonprofits only
pCfB2311 (SNR52p-gRNA.ADE2-SUP4t_natMX)

Plasmid

#83947

Purpose

gRNA cassette-carrying vector with natMX marker

Depositor

Irina Borodina

Publication

Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

Insert

gRNA targeting ADE2 gene

Expression

Yeast

Available since

Oct. 27, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-CaMKII-PinkyCaMP

Plasmid

#232858

Purpose

An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under CaMKII promoter

Depositor

Olivia Masseck

Publication

Fink et al Nat Methods. 2026 Apr 24. doi: 10.1038/s

Insert

PinkyCaMP

Use

AAV

Expression

Mammalian

Promoter

CaMKII

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pHybrid-Electra2

Plasmid

#179475

Purpose

Expresses Electra2 in bacterial cells and Electra2 and mScarlet in mammalian cells

Depositor

Kiryl Piatkevich

Publication

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Inserts

Electra2
mScarlet-I

Expression

Bacterial and Mammalian

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Lin28

Plasmid

#60345

Purpose

Mammalian Expression, Lentiviral

Depositor

Rudolf Jaenisch

Publication

Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

Insert

Lin28 (Lin28a Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Minimal CMV + TetO

Available since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
EYFP-Rab7

Plasmid

#20164

Depositor

Xiaowei Zhuang

Publication

Lakadamyali et al Cell. 2006 Mar 10. 124(5):997-1009.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rab7 (RAB7A Canis lupus familiaris)

Expression

Mammalian

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pMusheLL

Plasmid

#47023

Purpose

Mouse PV L1 and L2, Bicistronic, too large to self-package

Depositor

John Schiller

Publication

Handisurya et al Virology. 2012 Nov 25;433(2):385-94. doi

Insert

Mu L1+L2

Expression

Mammalian

Promoter

CMV

Available since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRVdGL-4Flpo

Plasmid

#98040

Purpose

Second-generation rabies viral vector genome

Depositor

Ian Wickersham

Publication

Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

Insert

Flpo

Expression

Mammalian

Promoter

CMV

Available since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti6/V5-DEST-MTHFD2

Plasmid

#31213

Depositor

Lynda Chin

Publication

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

MTHFD2 (MTHFD2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-DEST-ASF1B

Plasmid

#31205

Depositor

Lynda Chin

Publication

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

ASF1B (ASF1B Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
pCasperAUG-Gal4-X

Plasmid

#8378

Depositor

Leslie Vosshall

Publication

Vosshall et al Cell 2000 Jul 21;102(2):147-59.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Yeast Gal4 (GAL4 fused to first ~30 amino acids of Drosophila ADH, Budding Yeast)

Use

Drosophila p-element vector

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pCDNA3-Flag-SPRTN-wt

Plasmid

#110214

Purpose

Expression in mammalian cells of full-length wild-type SPRTN protein with a Flag-tag at N-terminus.

Depositor

Ivana Marinovic-Terzic, Kristijan Ramadan

Publication

Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

Insert

SPRTN (SPRTN Human)

Expression

Mammalian

Mutation

P296L (see depositor comments below)

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMS050

Plasmid

#91826

Purpose

Codon-optimized mScarlet-I donor for the SapTrap cloning system

Depositor

Bob Goldstein

Publication

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

mScarlet-I-C1

Use

CRISPR

Expression

Worm

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pRN3P_EGFP_m6A_Tracer

Plasmid

#139403

Purpose

in vitro transcription for mRNA

Depositor

Maria-Elena Torres-Padilla

Publication

Borsos et al Nature. 2019 May;569(7758):729-733. doi:

Insert

m6A-Tracer

Use

In vitro transcription

Tags

EGFP

Promoter

T3

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pdRBFC-B2-YN

Plasmid

#74721

Purpose

Express C-terminal YN fused flock house virus (FHV) B2 protein (2741-2956 nt) for dsRNA labeling

Depositor

Aiming Wang

Publication

Cheng et al Virology. 2015 Nov;485:439-51. doi: 10.1

Insert

Influenza A virus NS1 protein, partial

Use

Double-stranded rna labeling

Tags

HA and YFP N-terminal half domain

Expression

Plant

Promoter

CaMV 35S

Available since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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AAVS1-Neo-TRE-CMV-Cre-rtTA

Plasmid

#165457

Purpose

Tetracycline or doxycycline inducible CRE expression from AAVS1 locus

Depositor

Madeline Lancaster

Publication

Benito-Kwiecinski et al Cell. 2021 Apr 15;184(8):2084-2102.e19.

Insert

CRE

Expression

Mammalian

Promoter

Tight TRE promoter

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pZmUBQ:LUC

Plasmid

#131860

Purpose

Transient expression of firefly luciferase from the Zea mays ubiquitin promotor

Depositor

Paul Schulze-Lefert

Publication

Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

Insert

luciferase

Expression

Plant

Promoter

ZmUBQ

Available since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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