We narrowed to 4,952 results for: mcs

1. pcDNA6A-EGFR ECD (1-644)

   Plasmid

   #42666

   Depositor

   Mien-Chie Hung

   Article

   Hsu et al J Biol Chem. 2007 Apr 6;282(14):10432-40

   Insert

   EGFR ECD (1-644) (EGFR Human)
   

   Tags

   6xHis and Myc

   Expression

   Mammalian

   Available Since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 2xFLAG-2xSTREP TFDP2

   Plasmid

   #236445

   Purpose

   transient overexpression of TFDP2 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

   Insert

   TFDP2 (TFDP2 Human)
   

   Tags

   FLAG-FLAG-STREP-STREP

   Expression

   Mammalian

   Available Since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMarVF1

   Plasmid

   #99579

   Purpose

   Minitransposon delivery vector pMarVF1 (suicide vector) in V. fischeri ES114

   Depositor

   Mark Mandel

   Article

   Brooks JF et al Proc Natl Acad Sci U S A. 2014 Dec 2;111

   Insert

   V. fischeri mariner transposon (with erythromycin-resistance (ErmR) cassette)
   

   Use

   Suicide vector in v. fischeri es114

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJB02

   Plasmid

   #111959

   Purpose

   Expresses GST-GFP-DHFR; construct generated for pull-down and localization experiments

   Depositor

   Matthew Good

   Article

   Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

   Inserts

   mEGFP
   eDHFR
   

   Tags

   DHFR

   Expression

   Bacterial

   Mutation

   eDHFR;residues 2-159 and enhanced GFP with monome…

   Promoter

   T7

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET_RGG

   Plasmid

   #124929

   Purpose

   Expression of RGG domain from LAF-1 gene

   Depositor

   Daniel Hammer, Benjamin Schuster, Matthew Good

   Article

   Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

   Insert

   LAF-1 RGG domain (laf-1 Nematode)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   RGG domain

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSEM396 - mosTI unc-119 - NNN - operon - gfp - tbb-2

   Plasmid

   #182353

   Purpose

   GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Type

   Empty backbone

   Expression

   Worm

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXJ-His-PDCD1LG2

   Plasmid

   #232660

   Purpose

   transient expresses PD-L2 in mammalian cells

   Depositor

   Chen-Yang Shen

   Article

   Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

   Insert

   PDCD1LG2 (PDCD1LG2 Human)
   

   Tags

   His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJBL7045

   Plasmid

   #167216

   Purpose

   overexpression of CueR in E. coli for cell-free copper sensing

   Depositor

   Julius Lucks

   Article

   Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

   Insert

   CueR
   

   Expression

   Bacterial

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJBL7062

   Plasmid

   #167231

   Purpose

   cell-free cascaded copper sensor

   Depositor

   Julius Lucks

   Article

   Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

   Insert

   pCue-AKSIRV
   

   Use

   Synthetic Biology

   Tags

   6X His

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJBL7044

    Plasmid

    #167215

    Purpose

    overexpression of PbrR in E. coli for cell-free lead sensing

    Depositor

    Julius Lucks

    Article

    Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

    Insert

    PbrR
    

    Expression

    Bacterial

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJBL7061

    Plasmid

    #167230

    Purpose

    cell-free cascaded lead sensor

    Depositor

    Julius Lucks

    Article

    Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

    Insert

    pPbr-AKSIRV
    

    Use

    Synthetic Biology

    Tags

    6X His

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lyso-RA

    Plasmid

    #209875

    Purpose

    Dimerization dependent fluorescent protein RA anchored to cytosolic face of the lysosomal membrane with human LAMP1

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddRFP A
    

    Tags

    LAMP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA6A-EGFR ICD (645-1186)

    Plasmid

    #42667

    Depositor

    Mien-Chie Hung

    Article

    Hsu et al J Biol Chem. 2007 Apr 6;282(14):10432-40

    Insert

    EGFR ICD (645-1186) (EGFR Human)
    

    Tags

    6xHis and Myc

    Expression

    Mammalian

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pMXs-NKX2.2

    Plasmid

    #134670

    Purpose

    Retroviral expression of mouse Nkx2.2

    Depositor

    Marius Wernig

    Article

    Tanabe et al Development. 2022 Oct 15;149(20). pii: 2

    Insert

    Nkx2.2 (Nkx2-2 Mouse)
    

    Use

    Retroviral

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. human MTAP

    Plasmid

    #64077

    Purpose

    Expression of human 5'-methylthioadenosine phosphorylase

    Depositor

    Vern Schramm

    Article

    Guan et al Biochemistry. 2011 Nov 29;50(47):10408-1

    Insert

    5'-methylthioadenosine phosphorylase (MTAP Human, Synthetic)
    

    Tags

    Histidine tag and TEV

    Expression

    Bacterial

    Available Since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJBL7056

    Plasmid

    #167225

    Purpose

    orthogonal T7 RNAP sfGFP reporter

    Depositor

    Julius Lucks

    Article

    Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

    Insert

    pAKSIRV-sfGFP
    

    Use

    Synthetic Biology

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJL1 Aquamarine

    Plasmid

    #106285

    Purpose

    Expresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    Aquamarine
    

    Tags

    6xHis and TEV cleavage (N terminal on insert)

    Expression

    Bacterial

    Mutation

    T65S and H148G

    Promoter

    T7

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCOLA banana sensor sfGFP

    Plasmid

    #107367

    Purpose

    Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified banana DNA

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    banana-detecting toehold + sfGFP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE-UBC-TagRFP-T-ps

    Plasmid

    #178656

    Purpose

    Expression of palmitoylated TagRFP-T for live imaging of plasma membrane (protrusions, tunneing nanotubes), in mammalian cells

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al Proc Natl Acad Sci U S A. 2017 Nov 14;11

    Insert

    TagRFP-T with palmitoylation signal at N-terminus
    

    Use

    Lentiviral

    Tags

    Palmitoylation signal

    Promoter

    Ubc

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3 MDM2 S166D

    Plasmid

    #16234

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

    Insert

    MDM2 (MDM2 Human)
    

    Expression

    Mammalian

    Mutation

    Ser166 replaced by Asp

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only