We narrowed to 5,537 results for: mcs

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pcDNA3 MDM2 S166D S186D

Plasmid

#16236

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

Insert

MDM2 (MDM2 Human)

Expression

Mammalian

Mutation

Ser166 and Ser186 replaced by Asp

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
HSC1-GiP

Plasmid

#58254

Purpose

This self-inactivated retroviral vector has an internal human EF1alpha promoter driving the expression of a bicistronic EGFP ires Puromycin cassette

Depositor

James Ellis

Article

Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

Insert

Enhanced Green Fluorescence Protein, Puromycin resistance gene

Use

Retroviral

Expression

Mammalian

Promoter

Human EF1-alpha

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pMarVF1

Plasmid

#99579

Purpose

Minitransposon delivery vector pMarVF1 (suicide vector) in V. fischeri ES114

Depositor

Mark Mandel

Article

Brooks JF et al Proc Natl Acad Sci U S A. 2014 Dec 2;111

Insert

V. fischeri mariner transposon (with erythromycin-resistance (ErmR) cassette)

Use

Suicide vector in v. fischeri es114

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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(401) pAAV CMV-ZsGreen U6-gRNA

Plasmid

#163021

Purpose

Fluorescent reporter for Sa gRNA (Bsa1 sites)

Depositor

Tonia Rex

Article

Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

Insert

Zs Green

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pET_RGG

Plasmid

#124929

Purpose

Expression of RGG domain from LAF-1 gene

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

LAF-1 RGG domain (laf-1 Nematode)

Tags

His

Expression

Bacterial

Mutation

RGG domain

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pJL1-sfGFP-DQNAT

Plasmid

#198210

Purpose

pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing an optimal DQNAT sequon followed by a 6x-His tag

Depositor

Michael Jewett

Article

Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

Insert

sfGFP_DQNAT

Tags

6x His Tag and DQNAT glycosylation tag

Expression

Bacterial

Promoter

T7

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pNP1 sfGFP toehold switch

Plasmid

#107355

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 sfGFP trigger is co-expressed

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

toehold 7 + sfGFP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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JDW 1475 (pLenti-BLAST-GATA4-P2A-H2A-mCherry)

Plasmid

#233549

Purpose

A CMV driven human GATA4 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

GATA4 (GATA4 Human)

Use

Lentiviral

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pJB02

Plasmid

#111959

Purpose

Expresses GST-GFP-DHFR; construct generated for pull-down and localization experiments

Depositor

Matthew Good

Article

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Inserts

mEGFP
eDHFR

Tags

DHFR

Expression

Bacterial

Mutation

eDHFR;residues 2-159 and enhanced GFP with monome…

Promoter

T7

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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Lyso-RA

Plasmid

#209875

Purpose

Dimerization dependent fluorescent protein RA anchored to cytosolic face of the lysosomal membrane with human LAMP1

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddRFP A

Tags

LAMP1

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pSEM396 - mosTI unc-119 - NNN - operon - gfp - tbb-2

Plasmid

#182353

Purpose

GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA6A-EGFR ICD (645-1186)

Plasmid

#42667

Depositor

Mien-Chie Hung

Article

Hsu et al J Biol Chem. 2007 Apr 6;282(14):10432-40

Insert

EGFR ICD (645-1186) (EGFR Human)

Tags

6xHis and Myc

Expression

Mammalian

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3 2xFLAG-2xSTREP TFDP2

Plasmid

#236445

Purpose

transient overexpression of TFDP2 in mammalian cells

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

TFDP2 (TFDP2 Human)

Tags

FLAG-FLAG-STREP-STREP

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pXJ-His-PDCD1LG2

Plasmid

#232660

Purpose

transient expresses PD-L2 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

PDCD1LG2 (PDCD1LG2 Human)

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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human MTAP

Plasmid

#64077

Purpose

Expression of human 5'-methylthioadenosine phosphorylase

Depositor

Vern Schramm

Article

Guan et al Biochemistry. 2011 Nov 29;50(47):10408-1

Insert

5'-methylthioadenosine phosphorylase (MTAP Synthetic, Human)

Tags

Histidine tag and TEV

Expression

Bacterial

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pMXs-NKX2.2

Plasmid

#134670

Purpose

Retroviral expression of mouse Nkx2.2

Depositor

Marius Wernig

Article

Tanabe et al Development. 2022 Oct 15;149(20). pii: 2

Insert

Nkx2.2 (Nkx2-2 Mouse)

Use

Retroviral

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pJL1 Aquamarine

Plasmid

#106285

Purpose

Expresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

Aquamarine

Tags

6xHis and TEV cleavage (N terminal on insert)

Expression

Bacterial

Mutation

T65S and H148G

Promoter

T7

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCOLA banana sensor sfGFP

Plasmid

#107367

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified banana DNA

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

banana-detecting toehold + sfGFP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 MDM2 S166D

Plasmid

#16234

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

Insert

MDM2 (MDM2 Human)

Expression

Mammalian

Mutation

Ser166 replaced by Asp

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pSEM391 - mosTI unc-119 - Pmex-5 - NNN - tbb-2

Plasmid

#182351

Purpose

Germline expression cloning vector for mosTI(unc-119). Transgene inserted between Pmex-5 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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