Addgene

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T7-RelA(K310R)

Plasmid

#23250

Depositor

Warner Greene

Article

Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

Insert

NFkB RelA (RELA Human)

Use

Tags

T7 gp10

Expression

Mammalian

Mutation

K310R

Promoter

Available since

March 15, 2010

Availability

Academic Institutions and Nonprofits only
pCAG-Gdf2

Plasmid

#163595

Purpose

Expresses GDF-2 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Gdf2 (Gdf2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Gdf6

Plasmid

#163598

Purpose

Expresses GDF-6 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Gdf6 (Gdf6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLP-pR-himar-apraR

Plasmid

#199996

Purpose

Landing Pad with T7 RNAP circuit variant T15. pC194+Tn903 seed promoter. pR-himar transposase. In dap auxotrophic conjugation donor

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

Landing Pads

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM60 mUlk1 (wt) barcoded

Plasmid

#48799

Purpose

expresses wt Ulk1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

ULK1 (Ulk1 Mouse)

Use

Lentiviral

Tags

FLAG and barcode: GGATCC

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only
pCAG-Bmp2

Plasmid

#163587

Purpose

Expresses BMP-2 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp2 (Bmp2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
tetO-Myocd

Plasmid

#46033

Depositor

John Gearhart

Article

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

Myocd (Myocd Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

July 9, 2013

Availability

Academic Institutions and Nonprofits only

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pLJM60-S6K1(wt) barcoded

Plasmid

#48801

Purpose

expresses wt S6K1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

S6K1 (Rps6kb1 Rat)

Use

Lentiviral

Tags

FLAG and barcode: GGATCC

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only
pCAG-Bmp7

Plasmid

#163591

Purpose

Expresses BMP-7 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp7 (Bmp7 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT3-FLAG-KLF3

Plasmid

#49102

Purpose

Expresses KLF3 in mammalian cells

Depositor

Merlin Crossley

Article

Himeda et al Mol Cell Biol. 2010 Jul;30(14):3430-43.

Insert

Krüppel-Like Factor 3(KLF3)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Bmp5

Plasmid

#163589

Purpose

Expresses BMP-5 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp5 (Bmp5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTYF-sGFAP-IRES-tdTomato

Plasmid

#166920

Purpose

Empty plasmid backbone; includes IRES-tdTomato and sGFAP promoter

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

Type

Empty backbone

Use

Lentiviral

Tags

tdTomato

Expression

Mammalian

Mutation

Promoter

sGFAP

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Bmp10

Plasmid

#163593

Purpose

Expresses BMP-10 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp10 (Bmp10 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
T7-RelA(KR)

Plasmid

#23251

Depositor

Warner Greene

Article

Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

Insert

NFkB RelA (RELA Human)

Use

Tags

T7 gp10

Expression

Mammalian

Mutation

K218/221R, K310R

Promoter

Available since

March 15, 2010

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-hEFA6A

Plasmid

#206412

Purpose

Mammalian expression of hEFA6A

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Ueda et al FEBS Lett. 2013 Jun 5;587(11):1617-23. d

Insert

EFA6A (PSD Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaPH

Plasmid

#22930

Purpose

Mammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PH domain

Depositor

Alexandra Newton

Article

Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.

Insert

PHLPP1-deltaPH (PHLPP1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Includes 127-1205 aa. Deleted amino-terminal PH d…

Promoter

CMV

Available since

Feb. 3, 2010

Availability

Academic Institutions and Nonprofits only
pET21a - SodA

Plasmid

#89612

Purpose

Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase A protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Superoxide dismutase A

Use

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Mutation

Promoter

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1-hEFA6B

Plasmid

#206413

Purpose

Mammalian expression of hEFA6B

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Ueda et al FEBS Lett. 2013 Jun 5;587(11):1617-23. d

Insert

EFA6B (PSD4 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBroad-T7RNAP-apraR

Plasmid

#199993

Purpose

T7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) apraA

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

T7RNAP Circuit

Use

Tags

Expression

Bacterial

Mutation

copFnull

Promoter

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
T7-RelA(K221R)

Plasmid

#23248

Depositor

Warner Greene

Article

Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

Insert

NFkB RelA (RELA Human)

Use

Tags

T7 gp10

Expression

Mammalian

Mutation

K221R

Promoter

Available since

March 15, 2010

Availability

Academic Institutions and Nonprofits only