We narrowed to 2,808 results for: ALC
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Plasmid#47778PurposeExpresses enzymatically monobiotinylated partial RH2b ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised RH2b (partial)
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
Pf92-bio
Plasmid#47728PurposeExpresses enzymatically monobiotinylated full-length Pf92 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised Pf92
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
RON6-bio
Plasmid#47785PurposeExpresses enzymatically monobiotinylated full-length RON6 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised RON6
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin replaced…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pU6_empty-sgRNA(PP7)
Plasmid#232432Purposeempty gRNA backbone which contains the PP7 aptamer in the scaffold tetraloopDepositorInsertPP7-tagged sgRNA scaffold driven by human U6 promoter
UseCRISPRTagsExpressionMutationPromoterU6Available sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV_ABE8e–superNLS
Plasmid#232429PurposeExpression plasmid for the ABE8e base editor with superNLSDepositorInsertABE8e–superNLS
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6_HEK3+1T>A_(PP7-C4-Q1)
Plasmid#232437PurposepegRNA with optimized 3' modifications to facilitate a +1 T to A prime edit in the HEK3 locusDepositorInsert3'-PP7-tagged pegRNA for rd12 correction driven by human U6 promoter
UseCRISPRTagsExpressionMutationPromoterU6Available sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6_rd6_pegRNA_(PP7-C4-Q1)
Plasmid#232433PurposepegRNA with optimized 3' modifications to correct the rd6 mutationDepositorInsert3'-PP7-tagged pegRNA for rd6 correction driven by human U6 promoter
UseCRISPRTagsExpressionMutationPromoterU6Available sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6_rd6_nsgRNA(PP7)
Plasmid#232434PurposensgRNA for PE3b correction of the rd6 mutation, contains a PP7 in the scaffold tetraloopDepositorInsertPP7-tagged nicking gRNA (PE3b) for rd6 correction driven by human U6 promoter
UseCRISPRTagsExpressionMutationPromoterU6Available sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6_HEK3_nsgRNA(PP7)
Plasmid#232444PurposensgRNA for PE3 facilitation of a +1 T to A prime edit on the HEK3 locus, contains a PP7 in the scaffold tetraloopDepositorInsertPP7-tagged nicking gRNA (PE3) for HEK3+1t>a edit driven by human U6 promoter
UseCRISPRTagsExpressionMutationPromoterU6Available sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
MSP1-bio
Plasmid#47709PurposeExpresses enzymatically monobiotinylated full-length MSP1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised MSP1 (MSP1 Plasmodium falciparum, Synthetic)
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pU6_rd12_pegRNA_(PP7-C4-Q1)
Plasmid#232435PurposepegRNA with optimized 3' modifications to correct the rd12 mutationDepositorInsert3'-PP7-tagged pegRNA for rd12 correction driven by human U6 promoter
UseCRISPRTagsExpressionMutationPromoterU6Available sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6_rd12_nsgRNA(PP7)
Plasmid#232436PurposensgRNA for PE3b correction of the rd12 mutation, contains a PP7 in the scaffold tetraloopDepositorInsertPP7-tagged nicking gRNA (PE3b) for rd12 correction driven by human U6 promoter
UseCRISPRTagsExpressionMutationPromoterU6Available sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-H2B-Maroon-P2A-3xnls-Tq-Ca-FLITS
Plasmid#145027PurposeLentiviral vector for a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changes - co-expresses a nuclear markerDepositorInsert3xnls-Tq-Ca-FLITS
UseLentiviralTags3xnls-Tq-Ca-FLITS and H2B-MaroonExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
PF3D7_1136200-bio
Plasmid#47730PurposeExpresses enzymatically monobiotinylated full-length PF3D7_1136200 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised PF3D7_1136200
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin replaced…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
EBL1-bio
Plasmid#47792PurposeExpresses enzymatically monobiotinylated full-length EBL1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised EBL1
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
Rabbit Cav 2.2 W391A pMT2
Plasmid#58734Purposeexpression of rabbit CaV2.2 calcium channels with a mutation (W391) in the α1-interacting domain (AID) in the I-II loop of CaV2.2DepositorInsertCav2.2 (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationW391APromoterSV40Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
TLP-bio
Plasmid#47787PurposeExpresses enzymatically monobiotinylated full-length TLP ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised TLP
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
PF3D7_1431400-bio
Plasmid#47791PurposeExpresses enzymatically monobiotinylated full-length PF3D7_1431400 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised PF3D7_1431400
UseTagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLX304_miRFP670nano-P2A-ERM-gCam6f
Plasmid#214927PurposeExpress miRFP670nano P2A ERM-gCaMP6f (fluorescent proteins and genetic calcium sensor, gCaMP6f for normalizing factor and sensing calcium ions at cytosol exposed to ERM, respectively)DepositorInsertmiRFP670nano-P2A-ERM-gCam6f
UseLentiviralTagsExpressionMammalianMutationPromoterCMVpAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-hMICU1EF
Plasmid#199180PurposeThis plasmid is used to express a EF-hand disabled human MICU1 protein in E. coli. The protein is fused with a maltose binding protein in the N-terminus, and also contains a C-terminal His6 tag.DepositorInsertMICU1 (MICU1 Human)
UseTagsHis6 and MBP, Thrombin site, TEV siteExpressionMutationD421A, E432K, F433W, F453WPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-MCU-Flag-TetOFF
Plasmid#185657PurposeTet/dox-repressible expression of C-terminus Flag tagged human mitochondrial calcium uniporterDepositorInsertMitochondrial calcium uniporter (MCU Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterpTight TREAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSET-RCaMP1h
Plasmid#42874Purposea single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRubyDepositorInsertRed Fluorescent Calcium binding Protein
UseTagsEK (Enterokinase) Recognition Site, His Tag (6x),…ExpressionBacterialMutationPromoterT7Available sinceFeb. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pU6_B2M_sgRNA(PP7)
Plasmid#232438PurposesgRNA to facilitate a splice donor site disruption via adenine base editing in the B2M locus, contains a PP7 aptamer in the scaffold tetraloopDepositorInsertPP7-tagged gRNA for B2M disruption via splice donor consensus disruption via ABE8e or Cas9 driven by human U6 promoter
UseCRISPRTagsExpressionMutationPromoterU6Available sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-SomaGCaMP7
Plasmid#182942PurposeCre-dependent expression of cell body-targeted GCaMP7f, under CAG promoter. As bright as conventional GCaMP7f.DepositorInsertSoma-GCaMP7
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
elavl3:SomaGCaMP7f
Plasmid#158760PurposeCell body-targeted GCaMP7f, under HuC promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.DepositorInsertSomaGCaMP7
UseSynthetic BiologyTagsExpressionMutationPromoterHuC promoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FLEX-SomaGCaMP7
Plasmid#182941PurposeCre-dependent expression of cell body-targeted GCaMP7f, under EF1a promoter. As bright as conventional GCaMP7f.DepositorInsertSoma-GCaMP7
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-Soma-jGCaMP8f
Plasmid#182943PurposeCell body-targeted jGCaMP8f, under synapsin promoter.DepositorInsertSoma-jGCaMP8f
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-MCU-mCerulean
Plasmid#185809PurposeHuman mitochondrial calcium uniporter with C-terminus mCerulean tagDepositorInsertMitochondrial calcium uniporter (MCU Human)
UseLentiviralTagsmCeruleanExpressionMammalianMutationPromoterCMVAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-SomaGCaMP7
Plasmid#158759PurposeCell body-targeted GCaMP7f, under synapsin promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.DepositorInsertSomaGCaMP7
UseAAVTagsExpressionMammalianMutationPromotersynapsin promoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-SomaGCaMP6f2
Plasmid#158756PurposeCell body-targeted GCaMP6f, under synapsin promoter: GCaMP6f followed by a linker, and the EE-RR coiled-coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.DepositorInsertSomaGCaMP6f2
UseAAVTagsExpressionMammalianMutationPromotersynapsin promoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-SomaGCaMP6f2
Plasmid#158757PurposeCell body-targeted GCaMP6f, under CAG promoter: GCaMP6f followed by a linker, and the EE-RR coiled coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.DepositorHas ServiceAAV8 and AAV9InsertSomaGCaMP6f2
UseAAVTagsExpressionMammalianMutationPromoterCAG promoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-SomaGCaMP6f1
Plasmid#158755PurposeCell body-targeted GCaMP6f under synapsin promoter: GCaMP6f followed by a linker, the tail region of AnkyrinG, and an ER export motif (GCaMP6f-27-AnkTail-motif-ER2). Not as bright as SomaGCaMP6f2.DepositorInsertSomaGCaMP6f1
UseAAVTagsExpressionMammalianMutationPromotersynapsin promoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-nullCoChR-GCaMP6f-Kv2.1
Plasmid#158758PurposeCell body-targeted GCaMP6f, under synapsin promoter: nullCoChR (the optogenetic protein CoChR mutated to have 0 current), followed by GCaMP6f and the KV2.1 motif (nullCoChR-GCaMP6f-Kv2.1).DepositorInsertnullCoChR-GCaMP6f-Kv2.1
UseAAVTagsExpressionMammalianMutationPromotersynapsin promoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-FRB-MCU-Flag-TetOFF
Plasmid#185659PurposeTet/dox-repressible expression of N-terminus FRB tagged human mitochondrial calcium uniporter with C-terminus Flag epitopeDepositorInsertMitochondrial calcium uniporter (MCU Human)
UseLentiviralTagsFRB and FlagExpressionMammalianMutationPromoterpTight TREAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMJA149
Plasmid#128524PurposeExpresses mutant Ber e 1 in PichiaDepositorInsertmutant Ber e 1
UseTagsExpressionYeastMutationPromoterAOX1Available sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMJA151
Plasmid#128526PurposeExpresses mutant Ber e 1 in PichiaDepositorInsertmutant Ber e 1
UseTagsExpressionYeastMutationPromoterAOX1Available sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMJA159
Plasmid#128534PurposeExpresses mutant Ber e 1 in PichiaDepositorInsertmutant Ber e 1
UseTagsExpressionYeastMutationPromoterAOX1Available sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMJA157
Plasmid#128532PurposeExpresses mutant Ber e 1 in PichiaDepositorInsertmutant Ber e 1
UseTagsExpressionYeastMutationPromoterAOX1Available sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMJA156
Plasmid#128531PurposeExpresses mutant Ber e 1 in PichiaDepositorInsertmutant Ber e 1
UseTagsExpressionYeastMutationPromoterAOX1Available sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMJA155
Plasmid#128530PurposeExpresses mutant Ber e 1 in PichiaDepositorInsertmutant Ber e 1
UseTagsExpressionYeastMutationPromoterAOX1Available sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only