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Showing: 281 - 320 of 473 results

  5. pTFG005_Spike_Inducible_3xFlag

    Plasmid
    #191578
    Purpose
    Inducible expression of SARS-CoV-2 Spike protein with C-terminal 3x FLAG tag under TRE3G promoter and cloned into a 2nd generation lentiviral transfer plasmid (modified pLVX with Hygro resistance).
    Depositor
    Joshua Leonard
    Article
    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10
    Insert
    SARS-CoV-2 Spike Protein (S Synthetic)
    Use
    Lentiviral
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    191578-plasmid-map-sequence-id-442483

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  17. pHIE323-LPIV Puro-P2A-miRFP720 ZF6x12-C-DsRed-Express2(x6) phEF1α-BlastR

    Plasmid
    #201539
    Purpose
    Landing pad integration vector used to generate ZF6 synTF reporter line (HIE156), assembled with pPD1178 (destination vector), pHIE324-329 (TUPV1-6), pHIE280 (TUPV7), pPD1157 (linker)
    Depositor
    Joshua Leonard
    Article
    Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:
    Inserts
    PuromycinR-P2A-miRFP720
    ZF6x12-C-DsRed-Express2(x6)
    BlasticidinR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    None, ZF6x12(C)_YB_TATA Minimal Promoter, and hEF…
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    201539-plasmid-map-sequence-id-401324

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  20. pTFG027_Spike_sACE2-resistant_D614G_F486S_3xFlag

    Plasmid
    #191572
    Purpose
    Mutated SARS-CoV-2 Spike under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. Encodes F486S mutation. For expression and pseudotyping lentiviral particles.
    Depositor
    Joshua Leonard
    Article
    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10
    Insert
    SARS-CoV-2 Spike Protein (S Synthetic)
    Use
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    D614G and F486S (confers reduced binding affinity…
    Promoter
    CMV
    Available since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191572-plasmid-map-sequence-id-441975

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  21. L2088-Mut-GPA-NLuc-Biosensor-Retrovirus-TD138

    Plasmid
    #222876
    Purpose
    Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). Mixed WT/V84R Glycophorin A (GPA) scaffold.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA(V84R)-NanoLuc114-T2A-FKBP-GPA(WT)-NanoLuc11S
    Use
    Retroviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    FRB chain: V84R in GPA
    Promoter
    MSCV LTR
    Available since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    222876-plasmid-map-sequence-id-441621

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  22. pTFG011_Spike_Parental_D614G_3xFlag

    Plasmid
    #191571
    Purpose
    SARS-CoV-2 Spike protein under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. For expression and pseudotyping lentiviral particles.
    Depositor
    Joshua Leonard
    Article
    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10
    Insert
    SARS-CoV-2 Spike Protein (S Synthetic)
    Use
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    D614G mutation. Last 19 amino acids truncated.
    Promoter
    CMV
    Available since
    July 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    191571-plasmid-map-sequence-id-440477

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  23. pUC57-mini v2-HF24_F4

    Plasmid
    #195718
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#4 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195718-plasmid-map-sequence-id-385728

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  24. pUC57-mini v2-HF24_F20

    Plasmid
    #195734
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#20 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 16, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195734-plasmid-map-sequence-id-387873

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  25. pUC57-mini v2-HF24_F5

    Plasmid
    #195719
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#5 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 16, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195719-plasmid-map-sequence-id-387872

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  26. pUC57-mini v2-HF24_F12

    Plasmid
    #195726
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#12 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 16, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195726-plasmid-map-sequence-id-387868

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  27. pUC57-mini v2-HF24_F3

    Plasmid
    #195717
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#3 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 16, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195717-plasmid-map-sequence-id-387867

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  28. pUC57-mini v2-HF24_F24

    Plasmid
    #195738
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#24 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 16, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195738-plasmid-map-sequence-id-387865

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  29. pUC57-mini v2-HF24_F7

    Plasmid
    #195721
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#7 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 16, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195721-plasmid-map-sequence-id-387861

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  30. pUC57-mini v2-HF24_F8

    Plasmid
    #195722
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#8 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 16, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195722-plasmid-map-sequence-id-387859

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  31. pUC57-mini v2-HF24_F14

    Plasmid
    #195728
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#14 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 16, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195728-plasmid-map-sequence-id-387858

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  32. pUC57-mini v2-HF24_F13

    Plasmid
    #195727
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#13 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195727-plasmid-map-sequence-id-385601

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  33. pUC57-mini v2-HF24_F22

    Plasmid
    #195736
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#22 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195736-plasmid-map-sequence-id-385599

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  34. pUC57-mini v2-HF24_F10

    Plasmid
    #195724
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#10 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195724-plasmid-map-sequence-id-385596

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  35. pUC57-mini v2-HF24_F6

    Plasmid
    #195720
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#6 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195720-plasmid-map-sequence-id-385595

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  36. pUC57-mini v2-HF24_F15

    Plasmid
    #195729
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#15 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195729-plasmid-map-sequence-id-385594

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  37. pUC57-mini v2-HF24_F17

    Plasmid
    #195731
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#17 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195731-plasmid-map-sequence-id-385593

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  38. pUC57-mini v2-HF24_F19

    Plasmid
    #195733
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#19 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195733-plasmid-map-sequence-id-385592

    Enlarge

  39. pUC57-mini v2-HF24_F2

    Plasmid
    #195716
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#2 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195716-plasmid-map-sequence-id-385591

    Enlarge

  40. pUC57-mini v2-HF24_F11

    Plasmid
    #195725
    Purpose
    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.
    Depositor
    Eric Cantor
    Article
    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2
    Insert
    Fragment#11 of 24 fragment splits in lacI/lacZ cassette
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    195725-plasmid-map-sequence-id-385589

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Showing: 281 - 320 of 473 results