Addgene

We narrowed to 358 results for: gag pol

Showing: 281 - 320 of 358 results

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pQE-9-RTXMCR-TLY-X2D-C

Plasmid

#225975

Purpose

Repeats-In-Toxin (RTX) protein consensus sequence GGAGDDTLY repeated nine times with C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXMCR-TLY-X2D-C

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pQE-9-RTXMCR-TLY-X2E-C

Plasmid

#225976

Purpose

Repeats-In-Toxin (RTX) protein consensus sequence GGAGEDTLY repeated nine times with C-terminal Capping Domain

Depositor

Danielle Mai

Article

Chang et al Polym Chem. 2024. doi: https://doi.org/1

Insert

RTXMCR-TLY-X2E-C

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1-CENPB

Plasmid

#120208

Purpose

gRNA targeting the stem loop of CENPB poly(A) site

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB poly(A) site

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shLuc.mKO2

Plasmid

#85224

Purpose

shLuc (Target TTACGCTGAGTACTTCGA) for silencing luciferase gene as a control and express monomeric Kusabira-Orange2.

Depositor

Sandra Martha Gomes Dias

Article

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Insert

Firefly Luciferase

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shGBP1.2.mKO2

Plasmid

#85210

Purpose

TRCN0000116120 (Target TGAGACGACGAAAGGCATGTA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.

Depositor

Sandra Martha Gomes Dias

Article

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Insert

GBP1 (guanylate binding protein 1) (GBP1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-shRNA-ctrl

Plasmid

#85741

Purpose

shRNA ctrl

Depositor

Hongjun Song

Article

Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

Insert

shRNA ctrl

Use

AAV

Tags

EYFP

Expression

Mutation

Promoter

Available since

April 22, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-shRNA-Tet1

Plasmid

#85742

Purpose

shRNA against mouse Tet1

Depositor

Hongjun Song

Article

Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

Insert

shRNA Tet1

Use

AAV

Tags

EYFP

Expression

Mutation

Promoter

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB8622

Plasmid

#126912

Purpose

Plasmid containing gRNA expression cassette targeting ADE2 in Saccharomyces cerevisiae

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

ADE2 gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO-cfNHERF2_4

Plasmid

#195227

Purpose

shRNA to canine NHERF2.

Depositor

Keith Mostov, David Bryant

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

NHERF2 (SLC9A3R1 canine)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO-cfNHERF2_5

Plasmid

#195228

Purpose

shRNA to canine NHERF2.

Depositor

Keith Mostov, David Bryant

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

NHERF2 (SLC9A3R1 canine)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pMpGE_En03-sgRNA-Target1 (MpRTN1)

Plasmid

#186291

Purpose

Gateway entry vector for sgRNA (target 1: MpRTN1). Transient expression of sgRNA (Target 1: MpRTN1) for MpRTN1 in plant cells

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

sgRNA-Target1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGE010-sgRNA-Target1 (MpRTN1)

Plasmid

#186295

Purpose

Binary vector for CRISPR/Cas9 (target 1: MpRTN1) in plants (for Agrobacterium-mediated genetic transformation)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

sgRNA-Target1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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B53

Plasmid

#74006

Purpose

gRNA for B108

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

gRNA

Use

CRISPR

Tags

none

Expression

Mutation

Promoter

U6

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-PM-BAF180-SH3

Plasmid

#41933

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BAF180

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only
AAV-shRNA_Tet3

Plasmid

#85740

Purpose

shRNA against mouse Tet3

Depositor

Hongjun Song

Article

Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

Insert

Tet3 (Tet3 Mouse)

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB8792

Plasmid

#126910

Purpose

Template for PCR amplification of ADE2 gRNA biobrick for targeting Saccharomyces cerevisiae ADE2 gene

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

ADE2 gRNA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB5191

Plasmid

#126911

Purpose

Plasmid containing gRNA expression cassettes targeting the X-4 and XII-5 integration sites in Saccharomyces cerevisiae

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

X-4 and XII-5 gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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CAG-EGFP-Otx2shRNA2

Plasmid

#73982

Purpose

Otx2 shRNA2 (based on mir155) was inserted into the 3'UTR of EGFP.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

EGFP-Otx2-shRNA2

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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LEPG_shSik3.308

Plasmid

#125878

Purpose

Retroviral expression of mouse Sik3 shRNA with GFP and puromycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Insert

shRNA targeting Sik3

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

MSCV-LTR

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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TLCV2-sgHPDL #3

Plasmid

#174165

Purpose

knock out HPDL in human cell lines

Depositor

Michael Pacold

Article

Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

Insert

sgRNA against HPDL

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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CAG-mCherry-Otx2shRNA2

Plasmid

#73980

Purpose

Otx2 shRNA2 (mir155 backbone) was inserted into the 3'UTR of mCherry.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

shRNA that targets the mouse Otx2 gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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Cdk12_intron3_sg2_pX330

Plasmid

#127174

Purpose

sgRNA that cuts within intron 3 of mouse Cdk12 genomic locus in pX330 backbone

Depositor

Phil Sharp

Article

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

Mouse Cdk12 Intron 3 sgRNA

Use

CRISPR and Mouse Targeting

Tags

Expression

Mutation

Promoter

U6 Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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Cdk12_intron4_sg1_pX458

Plasmid

#127175

Purpose

sgRNA that cuts within intron 4 of mouse CDK12 genomic locus in pX458 backbone

Depositor

Phil Sharp

Article

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

Mouse Cdk12 Intron 4 sgRNA

Use

CRISPR and Mouse Targeting

Tags

Expression

Mutation

Promoter

U6 Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pXPR_023d-sgCiBIRC6-1

Plasmid

#202447

Purpose

Expression of a CRISPRi guide targeting BIRC6

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 gRNA (BIRC6 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003-sgBIRC6-4

Plasmid

#202438

Purpose

Expression of a guide RNA targeting BIRC6

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 gRNA (BIRC6 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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gRNA-ura-HYB

Plasmid

#64330

Purpose

ura3 deletion gRNA cassette carried by pRS42H

Depositor

Yong-Su Jin

Article

Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

Insert

gBlock product of ura3 deletion gRNA cassette

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
pGM63

Plasmid

#220169

Purpose

CRISRPi guide RNA against TOMM70A

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TOMM70A (sgRNA) (TOMM70 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV.CBA.YFP.miR-E_shSrpk3-1

Plasmid

#180400

Purpose

Producing AAV that encodes mouse Srpk3 shRNA-1 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shSrpk3-1 (Srpk3 Mouse)

Use

AAV and RNAi

Tags

Expression

Mutation

Promoter

CBA

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV.CBA.YFP.miR-E_shSrpk3-3

Plasmid

#180402

Purpose

Producing AAV that encodes mouse Srpk3 shRNA-3 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shSrpk3-3 (Srpk3 Mouse)

Use

AAV and RNAi

Tags

Expression

Mutation

Promoter

CBA

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330_gRNA_IG-DMR-1

Plasmid

#159931

Purpose

Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

Depositor

Anton Wutz

Article

Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

Insert

gRNA mouse IG-DMR

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U6, CBh

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330_gRNA_IG-DMR-2

Plasmid

#159932

Purpose

Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

Depositor

Anton Wutz

Article

Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

Insert

gRNA mouse IG-DMR

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U6, CBh

Available since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.23-CD69 CaRE3 (plus strand)

Plasmid

#91844

Purpose

Luciferase reporter for CD69 enhancer (IGI-P0621)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

CD69 CaRE3 (plus strand) (CD69 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Missing 1 base from 13 base polyT tract beginning…

Promoter

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.23-CD69 CaRE3 (minus strand)

Plasmid

#91845

Purpose

Luciferase reporter for CD69 enhancer (IGI-P0622)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

CD69 CaRE3 (minus strand) (CD69 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Missing 1 base from 13 base polyT tract beginning…

Promoter

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-MCS-shCHD5 #1

Plasmid

#68876

Purpose

CHD5 shRNA expressed from Adeno-associated viral (AAV) vector

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5

Use

AAV and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only
pLKO.1P MUTYH_1

Plasmid

#160785

Purpose

Suppress MUTYH

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shMUTYH_1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV.CBA.YFP.miR-E_shTgm5-1

Plasmid

#180394

Purpose

Producing AAV that encodes mouse Tgm5 shRNA-1 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shTgm5-1 (Tgm5 Mouse)

Use

AAV and RNAi

Tags

Expression

Mutation

Promoter

CBA

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1P RTEL1_1

Plasmid

#160799

Purpose

Suppress RTEL1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shRTEL1_1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1P PRIM2_1

Plasmid

#160795

Purpose

Suppress PRIM2

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPRIM2_1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1P NTHL1_2

Plasmid

#160788

Purpose

Suppress NTHL1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shNTHL1_2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1P EXO5_2

Plasmid

#160784

Purpose

Suppress EXO5

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shEXO5_2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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