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We narrowed to 475 results for: piggybac vectors

Showing: 281 - 320 of 475 results

  3. PB-CT3G-CERP2-MG-IRES2-mCherry

    Plasmid
    #175507
    Purpose
    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mCherry (CMVe-hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G-mCMV
    Available since
    Oct. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    175507-plasmid-map-sequence-id-345876

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  5. PB-CT3G-ERP2-MG-IRES2-mNeonGreen

    Plasmid
    #175506
    Purpose
    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G-mCMV
    Available since
    Oct. 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    175506-plasmid-map-sequence-id-345872

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  7. pPB.DEST-Illusia(YYEE)

    Plasmid
    #222594
    Purpose
    Piggybac expression vector with a phospho-mimetic mutant (YYEE) of the Illusia FRET reporter for integrin phosphorylation
    Depositor
    Johanna Ivaska
    Article
    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038
    Insert
    Illusia(YYEE)
    Use
    Transposon-based stable expression
    Tags
    Expression
    Mammalian
    Mutation
    Mutations in the NPxY sites equivolent to Y783E, …
    Promoter
    CAG
    Available since
    May 29, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    222594-plasmid-map-sequence-id-450070

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  8. pPB.DEST-Illusia(Y783F)

    Plasmid
    #222596
    Purpose
    Piggybac expression vector with a phospho-mutant (Y783F) variant of the Illusia FRET reporter for integrin phosphorylation
    Depositor
    Johanna Ivaska
    Article
    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038
    Insert
    Illusia(Y783F)
    Use
    Transposon-based stable expression
    Tags
    Expression
    Mammalian
    Mutation
    Mutation in the NPxY(783) site equivolent to Y783…
    Promoter
    CAG
    Available since
    May 29, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    222596-plasmid-map-sequence-id-450084

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  9. pPB.DEST-Illusia(Y795F)

    Plasmid
    #222598
    Purpose
    Piggybac expression vector with a phospho-mutant (Y795F) variant of the Illusia FRET reporter for integrin phosphorylation
    Depositor
    Johanna Ivaska
    Article
    Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038
    Insert
    Illusia(Y795F)
    Use
    Transposon-based stable expression
    Tags
    Expression
    Mammalian
    Mutation
    Mutation in the NPxY(795) site equivolent to Y795…
    Promoter
    CAG
    Available since
    May 29, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    222598-plasmid-map-sequence-id-450087

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  13. PB/pHSP-tTA-TRE-tTA-pEF1α-PuroR

    Plasmid
    #206213
    Purpose
    PiggyBac-based transposon vector plasmid which encodes an expression unit of tetracycline-responsive transactivator under control of HSP promoter with transcriptional amplification
    Depositor
    Masamichi Kamihira
    Article
    Kitano et al Cytotechnology. 2021 Jun;73(3):353-362.
    Insert
    tetracycline-responsive transactivator
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    206213-plasmid-map-sequence-id-409623

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  16. BII-gR-PnGW

    Plasmid
    #133355
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications), co-expressed with GFP-NLS
    Depositor
    Blair Madison
    Article
    Fischer et al Am J Physiol Gastrointest Liver Physiol.
    Insert
    GFP-NLS
    Use
    Tags
    HA-tagged GFP
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133355-plasmid-map-sequence-id-267392

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  17. LiOn*-CAG∞RFP

    Plasmid
    #154019
    Purpose
    Vector based on the LiOn integration-coupled translational switch and devoid of TTAA sequences, expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase
    Depositor
    Jean Livet
    Article
    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d
    Insert
    mRFP1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    All TTAA in vector backbone were replaced by sile…
    Promoter
    CAG
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    154019-plasmid-map-sequence-id-308117

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  19. pB-Tet-Off_FLEX_Luc-P2A-H2A-mCherry (JDW 970)

    Plasmid
    #229824
    Purpose
    A PiggyBac vector with a cre-dependent dual luciferase / nuclear mCherry reporter. In the presence of cre, this tet-off vector will be ubiquitously expressed in the absence of dox/tet
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.
    Insert
    Luciferase-P2A-H2A-mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229824-plasmid-map-sequence-id-460485

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  21. LZ10 PBREBAC-H2BHalo

    Plasmid
    #91564
    Purpose
    piggybac based mammalian expression vector for histone H2B HaloTag protein. Uses the EF1 promoter, confers Amp resistance in bacteria, and carrying NeoR for mammalian selection.
    Depositor
    James Zhe Liu
    Article
    Li et al Elife. 2016 Aug 3;5. pii: e17056. doi: 1
    Insert
    histone H2B-HaloTag
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    June 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    91564-plasmid-map-sequence-id-180643

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  33. ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-hSHH

    Plasmid
    #179586
    Purpose
    ePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette with SHH expression (Red module-dsRed/Green module-NeonGreen T2A SHH)
    Depositor
    Ali Hemmati Brivanlou
    Article
    De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:
    Insert
    NLS-NG-T2A-hSHH
    Use
    Piggybac
    Tags
    NeonGreen
    Expression
    Mutation
    Promoter
    TRE
    Available since
    Feb. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    179586-plasmid-map-sequence-id-354597

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  36. BII-gR-BtW-eSpCas9

    Plasmid
    #133358
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression
    Depositor
    Blair Madison
    Article
    Fischer et al Am J Physiol Gastrointest Liver Physiol.
    Insert
    eSpCas9
    Use
    Tags
    Flag-tagged eSpCas9
    Expression
    Mammalian
    Mutation
    eSpCas9 mutations
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    133358-plasmid-map-sequence-id-269429

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  39. JPF0510

    Plasmid
    #124001
    Purpose
    Encodes pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pEF1a-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124001-plasmid-map-sequence-id-252358

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  40. BII-gR-PtW-eSpCas9

    Plasmid
    #133357
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression
    Depositor
    Blair Madison
    Article
    Fischer et al Am J Physiol Gastrointest Liver Physiol.
    Insert
    eSpCas9
    Use
    Tags
    HA-tagged eSpCas9
    Expression
    Mammalian
    Mutation
    eSpCas9 mutations
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    133357-plasmid-map-sequence-id-267549

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Showing: 281 - 320 of 475 results