-
Plasmid#195101PurposeVector to introduce human CTCF cDNA tagged with eGFP and two AIDs in the cells. Blasticidin selection (2A-fusion). Auxin-inducible degron system.DepositorInsertCTCF (CTCF Human)
UseTagsAID, HA, and eGFPExpressionMammalianMutationPromoterpCAGGSAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3290
Plasmid#144766PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCTCF (CTCF Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_NPTN_CLUAP1
Plasmid#205869PurposeExpress mEGFP-tagged fusion protein, NPTN_CLUAP1 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CTCF
Plasmid#116728PurposeLentiviral expression of CTCFDepositorInsertCTCF (CTCF Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_CTCF_WT
Plasmid#81789PurposeGateway Donor vector containing CTCF , part of the Target Accelerator Plasmid Collection.DepositorInsertCTCF (CTCF Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGfa2(ABDx3)-nLac
Plasmid#53133Purposeexpression of nuclear LacZ driven by Gfa2 promoter with 3 additional copies of the gfa28 ABD segment; has about 100 times higher activity than pGfa2-nLac in transfected cellsDepositorInsertGfa2 (GFAP Human)
UseMouse TargetingTagsLacZ, NLS, and mP1ExpressionMammalianMutation-2163 to +47, with 3 copies of 3 copies of the gf…Promoterhuman Gfa2Available sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
Ankyrin-B scFv [N105/17]
Plasmid#190524PurposeMammalian Expression of Ankyrin-B scFV. Derived from hybridoma N105/17.DepositorInsertAnkyrin-B (Homo sapiens) recombinant scFV (ANK2 Mouse)
UseTagsHA, Sortase, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ankyrin-B scFv [N105/13]
Plasmid#190523PurposeMammalian Expression of Ankyrin-B scFV. Derived from hybridoma N105/13.DepositorInsertAnkyrin-B (Homo sapiens) recombinant scFV (ANK2 Mouse)
UseTagsHA, Sortase, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGfa(B3)-nLac
Plasmid#53132Purposeexpression of nuclear LacZ driven by Gfa2 promoter with 3 additional B sites, has about 75 times higher activity than pGfa2-nLac in transfected cellsDepositorInsertGfa2 (GFAP Human)
UseMouse TargetingTagsLacZ, NLS, and mP1ExpressionMammalianMutation-2163 to +47, with 3 copies of the gfa2 B segment…Promoterhuman Gfa2Available sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_CTCF_p.R377H
Plasmid#81566PurposeGateway Donor vector containing CTCF , part of the Target Accelerator Plasmid Collection.DepositorInsertCTCF (CTCF Human)
UseGateway entry vectorTagsExpressionMutationR377HPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_CTCF_p.N568K
Plasmid#81612PurposeGateway Donor vector containing CTCF , part of the Target Accelerator Plasmid Collection.DepositorInsertCTCF (CTCF Human)
UseGateway entry vectorTagsExpressionMutationN568KPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_CTCF_p.R377C
Plasmid#81584PurposeGateway Donor vector containing CTCF , part of the Target Accelerator Plasmid Collection.DepositorInsertCTCF (CTCF Human)
UseGateway entry vectorTagsExpressionMutationR377CPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_CTCF_p.P552S
Plasmid#81514PurposeGateway Donor vector containing CTCF , part of the Target Accelerator Plasmid Collection.DepositorInsertCTCF (CTCF Human)
UseGateway entry vectorTagsExpressionMutationP552SPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Anti-Ankyrin-B [N105/17R]
Plasmid#177494PurposeMammalian Expression Plasmid of anti-Ankyrin-B (Human). Derived from hybridoma N105/17.DepositorInsertanti-Ankyrin-B (Homo sapiens) recombinant mouse monoclonal antibody (ANK2 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Anti-Ankyrin-B [N105/13R]
Plasmid#177493PurposeMammalian Expression Plasmid of anti-Ankyrin-B (Human). Derived from hybridoma N105/13.DepositorInsertanti-Ankyrin-B (Homo sapiens) recombinant mouse monoclonal antibody (ANK2 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Anti-Ankyrin-B [N105/17R-2b]
Plasmid#225410PurposeMammalian Expression Plasmid of anti-Ankyrin-B (Human) IgG2b R-mAb. Derived from hybridoma N105/17.DepositorInsertAnti-Ankyrin-B (Homo sapiens) recombinant (Mouse) monoclonal antibody. (ANK2 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-Ankyrin-B [N105/13R-2b]
Plasmid#219483PurposeMammalian Expression Plasmid of anti-Ankyrin-B scaffold protein (Human) subclass-switched IgG2b R-mAb. Derived from hybridoma N105/13.DepositorInsertAnti-Ankyrin-B scaffold protein (Homo sapiens) recombinant mouse monoclonal antibody. (ANK2 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-Ankyrin-B [N105/13-rat IgG2a] - Chimeric
Plasmid#227040PurposeMammalian expression plasmid of anti-Ankyrin-B (Homo sapiens) rat IgG2a R-mAb. Derived from hybridoma N105/13.DepositorInsertAnti-Ankyrin-B (Homo sapiens) recombinant mouse monoclonal antibody. (ANK2 Chimera: M. musculus (mouse)/R. norvegicus (rat))
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEMS1375
Plasmid#29176PurposePleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).DepositorInsertPle88 (GFAP Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSExpressionMutationPromoterAvailable sinceNov. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEMS1377
Plasmid#29178PurposePleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).DepositorInsertPle90 (GFAP Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSExpressionMutationPromoterAvailable sinceNov. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEMS1376
Plasmid#29177PurposeExpression of the eGFP reporter gene was not detected in the brain using eGFP. Untested in the eye.DepositorInsertPle89 (GFAP Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSExpressionMutationPromoterAvailable sinceNov. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEMS1121
Plasmid#29045PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorInsertPle90 (GFAP Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-Cre-NLSExpressionMutationPromoterAvailable sinceJan. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEMS1559
Plasmid#29264PurposePleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748) and de Leeuw et al., MTM, 2014 (PMID: 24761428).DepositorInsertPle88 (GFAP Human)
UsePleiades promoter project [sic, pleaides plieades]TagsIntron-LacZ-NLSExpressionMutationPromoterAvailable sinceOct. 21, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBiT3.1-N_COP1:1-731
Plasmid#211039PurposeMammalian expression of full-length human COP1. N-terminal HiBiT tag.DepositorInsertCOP1:M1-V713 (COP1 Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationwild typePromoterCMVAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL_WDR19:1-636
Plasmid#210933PurposeInsect expression of human WDR19 fragment M1 to G636. N-terminal 6X His tag with TEV cleavage.DepositorInsertWDR19:M1-G636 (WDR19 Human)
UseTagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBiT3.1-C_COP1:1-731
Plasmid#211156PurposeMammalian expression of full-length human COP1. C-terminal HiBiT tag.DepositorInsertCOP1:M1-V713 (COP1 Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL_COP1:386-731
Plasmid#210887PurposeInsect expression of human COP1 fragment A386 to V731. N-terminal 6X His tag with TEV cleavage.DepositorInsertCOP1:A386-V731 (COP1 Human)
UseTagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
Dynein-2_complex
Plasmid#132536PurposeFull length human (Hs) IFT dynein (dynein-2) complex for Sf9 expression. Contains His-ZZ-TEV-SNAPf-HC, WDR60, WDR34-Strep, LIC3, TCTEX-1, TCTEX1D2, LC8-1, LC8-2, TCTEX-3, Rbl-1, Rbl-2, LC8-like.DepositorInsertsUseTags8x His, Linker-TEV-linker-TEV, SNAPf, Strep, and …ExpressionInsectMutationCodon optimised for Sf9 expressionPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1-6AlanineHE1
Plasmid#165098Purposebacterial expression of a GST fusion of Human Uba1 with a 6alanine linker between the GST encoded by the plasmid and the inserted Uba1DepositorInsertUba1 (UBA1 Human)
UseTagsGST-6-alanineExpressionBacterialMutationNonePromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3XFlag-S2P
Plasmid#32966PurposeFor mammalian expression of human S2P with a 3x FLAG tagDepositorInsertS2P (MBTPS2 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
-
pHIS-GB1-BP1
Plasmid#133427PurposeHuman BP1 fragment of SREBF1 with GB1 solubility tag in vector for in vitro expression, with T7 promoter.DepositorInsertSREBF1 (SREBF1 Human)
UseTagsExpressionBacterialMutationSREBP1a amino acids 17-42 fused to GB1 solubilit…PromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
WDR5 (2H9N)
Plasmid#25229PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertWDR5_01 (WDR5 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ube1/PET21d
Plasmid#34965DepositorInsertUbe1 (UBA1 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
WDR5 (2H9P)
Plasmid#25231PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertWDR5_01 (WDR5 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
WDR5 (2H9M)
Plasmid#25228PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertWDR5_01 (WDR5 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
WDR5 (2H9O)
Plasmid#25230PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertWDR5_01 (WDR5 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-hIBA1a-GFP-miR124T
Plasmid#214147PurposeAAV vector to restrict GFP expression in microglia.DepositorInsertGFP
UseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGfa2-luc3
Plasmid#53129Purposeluciferase reporter for human gfa2 promoterDepositorInsertgfa2 promoter fragment (GFAP Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationcontains -2163 to +47 (the human gfa2 segment), w…Promotergfa2Available sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
WDR5 (2GNQ)
Plasmid#25489PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertWDR5 (WDR5 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
WDR5 (2H9L)
Plasmid#25227PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertWDR5_01 (WDR5 Human)
UseTagsHisExpressionBacterialMutationlacking residues 1–23PromoterAvailable sinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
3UR4
Plasmid#60121PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertWDR5WDR5:APC041-G05:C36415 (WDR5 Human)
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
APC041-G05
Plasmid#58759Purposebacterial expression of PDB 4O45DepositorInsertPDB:4O45 (WDR5 Human)
UseTagsHisExpressionBacterialMutationBacterial expression for structure determination;…PromoterT7Available sinceAug. 5, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV.GFA104.PI.eGFP.WPRE.bGH
Plasmid#100896PurposeAAV expression of EGFP in astrocytesDepositorHas ServiceAAV5InsertEGFP
UseAAVTagsExpressionMammalianMutationPromoterGFA104 (GFAP promoter)Available sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
WDR5
Plasmid#36900PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertWDR5 (WDR5 Human)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationmutation version of plasmid #60121 3UR4PromoterAvailable sinceAug. 8, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
4IA9
Plasmid#51320PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertWDR5 (WDR5 Human)
UseTags6xHisExpressionBacterialMutationTruncation: See CommentsPromoterT7Available sinceFeb. 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
4QL1
Plasmid#61942PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertWDR5:APC041-G05:C36415 (WDR5 Human)
UseTags6x His and TEV cleavage siteExpressionBacterialMutationSee commentsPromoterT7Available sinceMarch 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
His-Tev-UbE1
Plasmid#186804PurposeCodon-optimized bacterial expression plasmid. Expresses ubiquitin activating enzyme His-Tev-UbE1.DepositorInsertHis-Tev-UbE1 (UBA1 Human)
UseTagsHis6-TevExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-2xFLAG-SREBP-1a
Plasmid#26801DepositorInsertSREBP-1a (SREBF1 Human)
UseTags2xFLAGExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-2xFLAG-SREBP-1c
Plasmid#26802DepositorInsertSREBP-1c (SREBF1 Human)
UseTags2XFLAGExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only