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EGFP-P2A-APEX2-ChBP

Plasmid

#108879

Purpose

Expression of APEX2 fused to a nanobody against mCherry (includes green cytoplasmic reporter)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

EGFP-P2A-APEX2-ChBP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-2xML1N(7Q)

Plasmid

#67666

Purpose

Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain (mutant version), [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

mKate2-P2A-APEX2-ML1Nmut*2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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MmCavin4-APEX2-P2A-mKate2

Plasmid

#170524

Purpose

Vector for expression of MmCavin4-APEX2-P2A-mKate2 in mammalian cells. Parton lab clone EJF

Depositor

Rob Parton

Article

Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

Insert

Cavin4 (Cavin4 Mouse)

Use

Tags

APEX2-P2A-mKate2

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pQCXIP-LAMP1-EGFP-APEX2-V5

Plasmid

#236402

Purpose

Expresses a fluorescent lysosome-localized APEX2

Depositor

Jared Rutter

Article

Cunningham et al J Cell Sci. 2025 Mar 13:jcs.263693. doi:

Insert

LAMP1-EGFP-APEX2-V5

Use

Retroviral

Tags

APEX2, EGFP, and V5

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pQCXIP-EGFP-APEX2-V5-EEA1

Plasmid

#236403

Purpose

Expresses a fluorescent endosome-localized APEX2

Depositor

Jared Rutter

Article

Cunningham et al J Cell Sci. 2025 Mar 13:jcs.263693. doi:

Insert

EGFP-APEX2-V5-EEA1

Use

Retroviral

Tags

APEX2, EGFP, and V5

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pQCXIP-Pex3-EGFP-APEX2-V5

Plasmid

#236401

Purpose

Expresses a fluorescent peroxisome-localized APEX2

Depositor

Jared Rutter

Article

Cunningham et al J Cell Sci. 2025 Mar 13:jcs.263693. doi:

Insert

Pex3(fragment)-EGFP-APEX2-V5

Use

Retroviral

Tags

APEX2, EGFP, and V5

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pQCXIP-EGFP-ADRP-APEX2-V5

Plasmid

#236405

Purpose

Expresses a fluorescent lipid droplet-localized APEX2

Depositor

Jared Rutter

Article

Cunningham et al J Cell Sci. 2025 Mar 13:jcs.263693. doi:

Insert

EGFP-ADRP-APEX2-V5

Use

Retroviral

Tags

APEX2, EGFP, and V5

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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FUGW-RUSH-LAMP1-V5-APEX2

Plasmid

#228524

Purpose

Lentiviral vector, expresses Strep-KDEL-IRES-SBP-(Rat)LAMP1-V5-APEX2 in mammalian cells.

Depositor

Ginny Farias

Article

Li et al Nat Commun. 2024 Dec;15: 10829. doi: 10.

Insert

LAMP1 (Lamp1 Rat)

Use

Lentiviral

Tags

Streptavidin Binding Protein (SBP), Streptavidin-…

Expression

Mammalian

Mutation

Promoter

Ubc

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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OMM-Flag-APEX2-SOPP3

Plasmid

#229073

Purpose

Expresses APEX2-SOPP3 targeted to outer mitochondrial membrane

Depositor

Weijun Pan

Article

Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

Insert

Express APEX2-SOPP3 in outer mitochondrial membrane (APEX2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-APEX2-SOPP3-3xNLS

Plasmid

#229074

Purpose

expresses APEX2-SOPP3 in the mammalian nucleus

Depositor

Weijun Pan

Article

Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

Insert

Express APEX2-SOPP3 in the mammalian nucleus (APEX2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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COX8mls-Flag-APEX2-SOPP3

Plasmid

#229075

Purpose

Expresses APEX2-SOPP3 in the mitochondrial matrix

Depositor

Weijun Pan

Article

Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

Insert

Express APEX2-SOPP3 in the mitochondrial matrix (APEX2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-SRRD-V5-APEX2 (in pLEX_307)

Plasmid

#214921

Purpose

constitutive expression of SRRD fused to V5 and APEX2 - used for APEX2 proximity biotinylation

Depositor

Ophir Shalem

Article

Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

Insert

SRRD (SRRD Human)

Use

Lentiviral

Tags

V5-APEX2

Expression

Mutation

Promoter

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS4-V5-APEX2-NES

Plasmid

#178704

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of V5-APEX2-NES in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

V5-APEX2-NES

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD6-V5-APEX2-NES

Plasmid

#178700

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of V5-APEX2-NES in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

V5-APEX2-NES

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS2-V5-APEX2-NES

Plasmid

#178702

Purpose

AAV vector for Cre-dependent transgene expression of V5-APEX2-NES in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

V5-APEX2-NES

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS3-V5-APEX2-NES

Plasmid

#178703

Purpose

AAV vector for Flp-dependent transgene expression of V5-APEX2-NES in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

V5-APEX2-NES

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD2-V5-APEX2-NES

Plasmid

#178696

Purpose

AAV vector for Cre-dependent transgene expression of V5-APEX-NES in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

V5-APEX2-NES

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD3-V5-APEX2-NES

Plasmid

#178697

Purpose

AAV vector for Flp-dependent transgene expression of V5-APEX2-NES in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

V5-APEX2-NES

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD4-V5-APEX2-NES

Plasmid

#178698

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of V5-APEX2-NES in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

V5-APEX2-NES

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-VTKD5-V5-APEX2-NES

Plasmid

#178699

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of V5-APEX2-NES in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

V5-APEX2-NES

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge