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Is3xP3-mCherry

Plasmid

#220091

Purpose

Expresses in ISE6 (Ixodes scapularis) cells, heat inducible. Promoter consists of 3 Pax 6 sites from 3xP3 with a minimal IsHSP70 promoter. Plasmid backbone derived from pGL-4.79 [Rluc] (Promega)

Depositor

Monika Gulia-Nuss

Article

Pham et al iScience. 2024 Jul 8;27(8):110468. doi:

Insert

3 Pax6 binding sites from 3xP3 with minimal HSP70 promoter from Ixodes scapularis

Use

Tags

Expression

Mutation

Promoter

Is3xP3

Available since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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ptia1l-hsp-GFP

Plasmid

#177837

Purpose

Donor plasmid for knock-in of hsp70-EGFP using CRISPR-Cas9

Depositor

David Kingsley

Article

Wucherpfennig et al Nat Ecol Evol. 2022 Sep 1. pii: 10.1038/

Insert

tia1l-sgRNA target site-hsp70-EGFP

Use

CRISPR

Tags

EGFP

Expression

Mutation

Promoter

hsp70

Available since

Sept. 2, 2022

Availability

Academic Institutions and Nonprofits only

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5538 pUG2-SSA1

Plasmid

#1247

Depositor

Susan Lindquist

Article

Glover et al Cell 1998 Jul 10;94(1):73-82.

Insert

Ssa1 (SSA1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pcDNA3 JDM37-GFPnb-mCh

Plasmid

#235666

Purpose

Expresses designed activator of Hsp70 tagged with a GFP nano body and mCherry

Depositor

David Baker

Article

Zhang et al Cell Chem Biol. 2025 Feb 4:S2451-9456(25

Insert

JDM37-GFPnb-mCh (PMCH Human, Synthetic)

Use

Tags

GFP nanobody and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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6559 pJC25YDJ1 #7

Plasmid

#1245

Depositor

Susan Lindquist

Article

Glover et al Cell 1998 Jul 10;94(1):73-82.

Insert

Ydj1 (YDJ1 Budding Yeast)

Use

Tags

his tag

Expression

Bacterial

Mutation

Promoter

Available since

July 18, 2005

Availability

Academic Institutions and Nonprofits only
6377 H10-104b

Plasmid

#1248

Depositor

Susan Lindquist

Article

Glover et al Cell 1998 Jul 10;94(1):73-82.

Insert

Hsp104 (HSP104 Budding Yeast)

Use

Tags

his tag

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
6379 H10-K218T

Plasmid

#1250

Depositor

Susan Lindquist

Article

Glover et al Cell 1998 Jul 10;94(1):73-82.

Insert

Hsp104 (K218T) (HSP104 Budding Yeast)

Use

Tags

his tag

Expression

Bacterial

Mutation

K218T

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
6380 H10-K620T

Plasmid

#1251

Depositor

Susan Lindquist

Article

Glover et al Cell 1998 Jul 10;94(1):73-82.

Insert

Hsp104 (K620T) (HSP104 Budding Yeast)

Use

Tags

his tag

Expression

Bacterial

Mutation

K620T

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO GFP HSPA1L

Plasmid

#19484

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

HSPA1L (HSPA1L Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HSPA1L

Plasmid

#19457

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

HSPA1L (HSPA1L Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HSPA2

Plasmid

#19458

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

HSPA2 (HSPA2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pT7C-HSPA1L

Plasmid

#177660

Purpose

Recombinant protein synthesis of human HSPA1L

Depositor

Jeff Kuret

Article

Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

Insert

HSPA1L (HSPA1L Human)

Use

Tags

6xHis-Thrombin

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP HSPA2

Plasmid

#19485

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

heat shock 70kDa protein 2 (HSPA2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP HSPA14

Plasmid

#19488

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

HSPA14 (HSPA14 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pET/LEGH

Plasmid

#108226

Purpose

Bacterial expression of EGFP with N-terminal lanthanide binding tag and C-terminal His6 tag

Depositor

Didier Picard

Article

Bhattacharya et al Sci Rep. 2018 Feb 12;8(1):2801. doi: 10.

Insert

LBT-EGFP-His6

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7, lacO

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HSPA14

Plasmid

#19461

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

HSPA14 (HSPA14 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pST96/LBT-MCS

Plasmid

#108227

Purpose

Bacterial expression expression of protein with N-terminal lanthanide binding tag

Depositor

Didier Picard

Article

Bhattacharya et al Sci Rep. 2018 Feb 12;8(1):2801. doi: 10.

Insert

LBT

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7, lacO

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pGL 4.79-mCherry

Plasmid

#220137

Purpose

promoterless vector

Depositor

Monika Gulia-Nuss

Article

Pham et al iScience. 2024 Jul 8;27(8):110468. doi:

Type

Empty backbone

Use

Ixodes scapularis

Tags

Expression

Mutation

Promoter

No promoter

Available since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pKM44

Plasmid

#154153

Purpose

Firefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter

Depositor

Kevin Maringer

Article

Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

Insert

Firefly luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

Drosophila melanogaster minimal Hsp70 promoter

Available since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5 HSPA14

Plasmid

#19515

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

heat shock 70kDa protein 14 (HSPA14 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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