We narrowed to 1,093 results for: pcs2

1. pCS2 hSmad1-MM

   Plasmid

   #22990

   Depositor

   Edward De Robertis

   Article

   Pera et al Genes Dev. 2003 Dec 15. 17(24):3023-8.

   Insert

   Smad1 (SMAD1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S214A, S206A, S195A, S187A

   Promoter

   Available Since

   July 21, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pCS2 hSmad1-GM

   Plasmid

   #22992

   Depositor

   Edward De Robertis

   Article

   Fuentealba et al Cell. 2007 Nov 30. 131(5):980-93.

   Insert

   Smad1 (SMAD1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S210A ,T202A, S191A, S198A

   Promoter

   Available Since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pCS2-wt-EGFP

   Plasmid

   #191117

   Purpose

   An wt EGFP control for Zebrafish

   Depositor

   Han Xiao

   Article

   Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

   Insert

   enhanced green fluorescence protein
   

   Use

   Tags

   His tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCS2xVlk1-3xFlag

   Plasmid

   #172773

   Purpose

   mRNA synthesis for injection into embryos

   Depositor

   Edward De Robertis

   Article

   Ding et al Dev Biol. 2017 Jun 15;426(2):176-187. do

   Insert

   Vlk1 (pkdcc.1.S Frog)
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCS2-XTbx5-GR

   Plasmid

   #117248

   Purpose

   mRNA synthesis

   Depositor

   Gerald Thomsen

   Article

   Horb et al Development. 1999 Apr;126(8):1739-51.

   Insert

   Tbx5-GR (tbx5-b Frog)
   

   Use

   Xenopus expression

   Tags

   Expression

   Mutation

   chimeric protein with human glucocorticoid recep…

   Promoter

   Available Since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCS2-MT-mitfa

   Plasmid

   #81231

   Purpose

   expression of zebrafish mitfa

   Depositor

   James Lister, David Raible

   Article

   Lister et al Development. 1999 Sep;126(17):3757-67.

   Insert

   mitfa (mitfa Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   6 myc tags

   Expression

   Mammalian

   Mutation

   contains amino acids 5-412

   Promoter

   sCMV IE94 promoter

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCS2+HA-hSeparase

   Plasmid

   #33018

   Depositor

   Marc Kirschner

   Article

   Stemmann et al Cell. 2001 Dec 14;107(6):715-26.

   Insert

   Separase (ESPL1 Human)
   

   Use

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   simian CMV IE94

   Available Since

   Nov. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pCS2+ Zf ctnna1L344P

   Plasmid

   #185407

   Purpose

   Zebrafish ctnna1 bearing a point mutation in the vinculin binding site. For in vitro transcription (SP6) or expression through CMV

   Depositor

   Nicolas David

   Article

   Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

   Insert

   ctnna1 (ctnna1 Zebrafish)
   

   Use

   In vitro synthesis of mrna

   Tags

   Expression

   Mammalian

   Mutation

   changed Leucine 344 to Proline

   Promoter

   Available Since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCS2+/C-Halo

   Plasmid

   #184418

   Purpose

   vector backbone to clone insert downstream of Halo tag (Halo tag codon optimized for Xenopus), for mRNA synthesis

   Depositor

   Ann Miller

   Article

   Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do

   Type

   Empty backbone

   Use

   For mrna synthesis

   Tags

   Halo-tag

   Expression

   Mutation

   Codon optimized for expression in Xenopus laevis …

   Promoter

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCS2+/N-SNAPf

    Plasmid

    #184415

    Purpose

    vector backbone to clone insert downstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesis

    Depositor

    Ann Miller

    Article

    Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do

    Type

    Empty backbone

    Use

    For mrna synthesis

    Tags

    SNAPf-tag

    Expression

    Mutation

    Codon optimized for expression in Xenopus laevis …

    Promoter

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCS2+/C-SNAPf

    Plasmid

    #184416

    Purpose

    vector backbone to clone insert upstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesis

    Depositor

    Ann Miller

    Article

    Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do

    Type

    Empty backbone

    Use

    For mrna synthesis

    Tags

    SNAPf-tag

    Expression

    Mutation

    Codon optimized for expression in Xenopus laevis …

    Promoter

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. E12 2/5-pCS2

    Plasmid

    #20913

    Depositor

    Stephen Tapscott

    Article

    Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

    Insert

    E12 2/5 (TCF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pCS2-ER-EmEGFP

    Plasmid

    #169000

    Purpose

    Express ER-EmEGFP in zebrafish

    Depositor

    Noboru Mizushima

    Article

    Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

    Insert

    ER-EGFP (encoding the signal sequence of human calreticulin mRNA and EGFP-KDEL) (CALR Synthetic, Human)
    

    Use

    Unspecified

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available Since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LRP6 dC-pCS2

    Plasmid

    #27258

    Depositor

    Xi He

    Article

    Tamai et al Nature. 2000 Sep 28. 407(6803):530-5.

    Insert

    low density lipoprotein receptor-related protein 6 (LRP6 Human)
    

    Use

    Xenopus injection

    Tags

    Expression

    Mammalian

    Mutation

    carboxyl intracellular region deleted Residue 14…

    Promoter

    Available Since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pCS2TAL3-DDD

    Plasmid

    #48637

    Purpose

    Expression of TALEN proteins with FokI-DDD domain

    Depositor

    David Grunwald

    Article

    Hu et al PLoS One. 2013 May 7;8(5):e63074. doi: 1

    Type

    Empty backbone

    Use

    TALEN

    Tags

    Flag

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCS2TAL3-RRR

    Plasmid

    #48636

    Purpose

    Expression of TALEN proteins with FokI-RRR domain

    Depositor

    David Grunwald

    Article

    Hu et al PLoS One. 2013 May 7;8(5):e63074. doi: 1

    Type

    Empty backbone

    Use

    TALEN

    Tags

    HA-1 and HA-2

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCS2z-tal1

    Plasmid

    #164656

    Purpose

    for the synthesis of zebrafish tal1 mRNA

    Depositor

    Didier Stainier

    Article

    Mattonet et al Sci Adv. 2022 Sep 2;8(35):eabn2082. doi:

    Insert

    tal1 (tal1 Zebrafish)
    

    Use

    Mrna synthesis

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCS2 xBtg-X

    Plasmid

    #24964

    Depositor

    Edward De Robertis

    Article

    Kuroda et al Genes Dev. 2005 May 1. 19(9):1022-7.

    Insert

    Btg-X (MGC76328.S Frog)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 18, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pCS2+5'MT-wbp2nl

    Plasmid

    #172467

    Purpose

    expresses myc-tagged version of wild-type protein

    Depositor

    Sally Moody

    Article

    Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

    Insert

    wbp2nl
    

    Use

    Xenopus expression

    Tags

    Myc

    Expression

    Mutation

    Promoter

    SP6

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCS2-hSeparase-wt

    Plasmid

    #33016

    Depositor

    Marc Kirschner

    Article

    Stemmann et al Cell. 2001 Dec 14;107(6):715-26.

    Insert

    Separase (ESPL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    simian CMV IE94

    Available Since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only