We narrowed to 11,913 results for: pcDNA

1. 3xHA-miniTurbo-NLS_pCDNA3

   Plasmid

   #107172

   Purpose

   expresses 3xHA-tagged miniTurbo in the mammalian nucleus

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   Available since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-AKAR4-NES

   Plasmid

   #64727

   Purpose

   Cytosol-targeted, FRET-based PKA activity reporter.

   Depositor

   Jin Zhang

   Article

   Herbst et al Mol Cell Biol. 2011 Oct;31(19):4063-75.

   Insert

   AKAR4-NES
   

   Use

   Tags

   Cerulean, NES, and cpVenus[E172]

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1.Gα11-LgB97

   Plasmid

   #134361

   Purpose

   Nanoluc complementation assay. Expression of Gα11 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gα11. Addition of the HA epitope at N terminus of Gα11.

   Depositor

   Julien Hanson

   Article

   Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

   Insert

   Gα11-LgB97 (GNA11 Human)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   T7

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1.Gα13-LgB106

   Plasmid

   #134364

   Purpose

   Nanoluc complementation assay. Expression of Gα13 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 106 and 107 of Gα13. Addition of the HA epitope at N term of Gα13.

   Depositor

   Julien Hanson

   Article

   Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

   Insert

   Gα13-LgB106 (GNA13 Human)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   T7

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Lyn-AKAR4

   Plasmid

   #61620

   Purpose

   Lipid raft-targeted FRET biosensor for monitoring PKA activity

   Depositor

   Jin Zhang

   Article

   Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

   Insert

   Lyn-AKAR4
   

   Use

   Tags

   Cerulean, N-terminal targeting sequence from Lyn …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-AKAP79-AKAR4

   Plasmid

   #181957

   Purpose

   Genetically encoded FRET-based PKA activity reporter fused to AKAP79.

   Depositor

   Jin Zhang

   Article

   Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

   Insert

   AKAP79-AKAR4 (AKAP5 Human)
   

   Use

   Tags

   AKAP79, Cerulean, HIS tag, and cpVenus(E172)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-GFP-BIRC3-SU

   Plasmid

   #160699

   Purpose

   Expresses GFP-BIRC3 with the short 3'UTR in mammalian cells

   Depositor

   Christine Mayr

   Article

   Lee et al Mol Cell. 2019 May 16;74(4):701-712.e9.

   Insert

   GFP-BIRC3 (BIRC3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-GFP-BIRC3-LU

   Plasmid

   #160700

   Purpose

   Expresses GFP-BIRC3 with the long 3'UTR in mammalian cells

   Depositor

   Christine Mayr

   Article

   Lee et al Mol Cell. 2019 May 16;74(4):701-712.e9.

   Insert

   GFP-BIRC3 (BIRC3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-FLAG-REL

   Plasmid

   #27253

   Depositor

   Thomas Gilmore

   Article

   Leeman et al Oncogene. 2008 Dec 4. 27(53):6770-81.

   Insert

   REL (REL Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 1, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA5/FRT/TO_H2B_HaloTag7

    Plasmid

    #169329

    Purpose

    Expression of H2B-HaloTag7 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag7 (H2BC21 Human)
    

    Use

    Tags

    HaloTag7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. p52 cFlag pcDNA3

    Plasmid

    #20019

    Depositor

    Stephen Smale

    Insert

    Nfkb2 (Nfkb2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Truncated at the p100 ligation site. It codes for…

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3-Myc-IQGAP1

    Plasmid

    #30118

    Depositor

    David Sacks

    Article

    Ho et al J Biol Chem. 1999 Jan 1. 274(1):464-70.

    Insert

    IQGAP1 (IQGAP1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA5/FRT/TO_H2B_HaloTag9_T2A_EGFP

    Plasmid

    #169332

    Purpose

    Nuclear expression of HaloTag9 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag9 (H2BC21 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.3-NL63-D14

    Plasmid

    #172666

    Purpose

    encodes NL63 spike protein, for pseudovirus production

    Depositor

    David Nemazee

    Article

    Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

    Insert

    HCNV63gp2 (HCNV63gp2 human coronavirus NL63)
    

    Use

    Lentiviral and Retroviral

    Tags

    NA

    Expression

    Mammalian

    Mutation

    c-terminal deletion of 14aa

    Promoter

    CMV

    Available since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-ExRai-AMPKAR

    Plasmid

    #192446

    Purpose

    Genetically encoded excitation-ratiometric fluorescent biosensor for monitoring AMPK activity in living cells.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    ExRai-AMPKAR
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-PTK7-VSV

    Plasmid

    #65250

    Purpose

    Expression of VSV tagged PTK7 in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Gartner et al PLoS One. 2014 Jan 7;9(1):e84472. doi: 1

    Insert

    PTK7 (PTK7 Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-PTK7DN-VSV

    Plasmid

    #65251

    Purpose

    Expression of VSV tagged dominant negative PTK7 in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Gartner et al PLoS One. 2014 Jan 7;9(1):e84472. doi: 1

    Insert

    PTK7DN (PTK7 Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    deleted AA 737-1071

    Promoter

    CMV

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA4/HisMax-Synectin

    Plasmid

    #35943

    Depositor

    Arie Horowitz

    Article

    Naccache et al Proc Natl Acad Sci U S A. 2006 Aug 22;10

    Insert

    Synectin (Gipc1 Mouse)
    

    Use

    Tags

    6xHIS and Xpress

    Expression

    Mammalian

    Mutation

    K111R

    Promoter

    CMV

    Available since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3-TLR5-CFP

    Plasmid

    #13019

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 5 (TLR5 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-N-Flag-Caspase-1

    Plasmid

    #75128

    Purpose

    Expresses N-terminal Flag-tagged Caspase-1 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    caspase 1 (Casp1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    P341S in Caspase-1 (please see depositor comments…

    Promoter

    CMV

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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