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Plasmid#90129PurposeGateway Mid-entry clone containing promoter sequence specific for nrpb1b driving expression of eGFPDepositorInsertnrp1bP1Egfp
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMEmafbbP1gfp
Plasmid#90128PurposeGateway Mid-entry clone containing promoter sequence specific for maffbb driving expression of eGFPDepositorInsertmaffbbP1Egfp
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
p5Enrp1bE1
Plasmid#90111Purpose5' Gateway entry clone containing endothelial specific enhancer sequence of nrp1bDepositorInsertnrp1bE1
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-mTurquoise2
Plasmid#98886PurposeProduces a fusion between mTurquoise2 and mNeonGreen. It can be used as a positive control for FRETDepositorInsertmNeonGreen-mTurquoise2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
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His6_MBP_FXR1P
Plasmid#198694PurposeExpression of human FXR1 protein in E. coliDepositorInsertFXR1P (FXR1 Human)
UseTagsMBPExpressionBacterialMutationPromoterT7Available sinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymNeongreen-URA3
Plasmid#168059PurposeReplaces Addgene Plasmid 125703. Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection; Contains the linker region at the 5’ end of the FPDepositorInsertymNeonGreen
UseTagsymNeonGreenExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
env8 reverse J1/13
Plasmid#99831PurposeGFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.DepositorInsertenv8 reverse J1/13
UseTagsGFPuvExpressionBacterialMutationJ1/13 sequence is reversedPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ac5-STABLE2-puro
Plasmid#208032PurposeEnables expression of 2 ORFs (with or without tags) and selection with puromycin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-puro with Actin5C promoter.DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCF806-reci
Plasmid#186711PurposeExpresses dox-controlled miR-E shRNAs (UT4GEPIR). Cloning recipient (reci).DepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
p5154
Plasmid#175390PurposeHigh Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts <15kb) into dual site mouse strains (Bxb-GT/GA)DepositorTypeEmpty backboneUseMouse Targeting; Cloning vector, to be used to de…TagsExpressionMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmcherry-LCa
Plasmid#53972PurposeExpresses the light chain of clathrinDepositorInsertclathrin light chain
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX330A_D10A-1x2
Plasmid#58772PurposeExpresses Cas9 nickase and gRNADepositorInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTags3xFLAGExpressionMammalianMutationD10A nickase-converting mutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHIV-H2B-eGFP
Plasmid#91776PurposeExpresses H2B protein tagged with eGFPDepositorInsertHuman Histone H2B / eGFP (H2BC11 Human)
UseLentiviralTagseGFPExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
Pink Flamindo
Plasmid#102356Purposebiosensor for cAMP in mammalian cellsDepositorInsertPink Flamindo
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV Puro
Plasmid#68469PurposeRetroviral plasmid with multiple cloning site and puromycin resistanceDepositorInsertPuromycin Resistance
UseRetroviralTagsExpressionMammalianMutationPromoterpGKAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;cldn5bP1Egfp
Plasmid#90158Purposecontains cldn5b specific gene promoter driving expression of eGFPDepositorInsertcldn5bP1Egfp (cldn5b Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNCS-mClover3
Plasmid#74236PurposeConstitutive expression of mClover3 in bacteriaDepositorHas ServiceCloning Grade DNAInsertmClover3
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tet-on circRNA Expression Vector
Plasmid#92351PurposeThe vector can ef_ciently produce a circRNA (circmCherry) from a split EGFP gene after Dox treatment.DepositorInsertmCherry and split EGFP
UseLentiviralTagsExpressionMammalianMutationPromoterpTetAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMK243 (Tet-OsTIR1-PURO)
Plasmid#72835PurposeExpresses OsTIR1 under the control of the Tet promoterDepositorInsertAAVS1-Tet-OsTIR1-PURO-AAVS1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Flag Rab12 Q101L
Plasmid#86788Purposemammalian expression of Rab12 Q101L, constitutively GTP-bound mutantDepositorInsertRab12 (RAB12 Human)
UseTagsFlagExpressionMammalianMutationQ101L, constitutively GTP-bound mutantPromoterCMVAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPLV_C1
Plasmid#186415PurposeEndogenous murine RNase inhibitor production for cell-free lysateDepositorInsertMurine RNase Inhibitor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDest-635
Plasmid#159582PurposeGateway Destination vector for protein expression in baculovirusDepositorTypeEmpty backboneUseTagsHis6ExpressionInsectMutationPromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIRE4-Bpa
Plasmid#155342PurposePlasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.DepositorInserthumanized EBpaRS/BstYam
UseTagsExpressionMammalianMutationPromoterPGK/U6Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-PTE2A
Plasmid#111773PurposeQuadcistronic construct: Co-express four genes by three 2A peptides -- P2A, T2A and E2ADepositorTypeEmpty backboneUseCloning intermediateTagsExpressionMutationPromoterT7Available sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGWB508-cTP-sfGFP
Plasmid#213863PurposeBinary vector for the expression of sfGFP flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)DepositorInsertPlastid transit peptide fused sfGFP
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Apex2-Venus-CAAX
Plasmid#168511PurposeExpression of Apex2 fused to Venus-CAAX for targeting to plasma membrane. Useful for correlated light-eletron microscopy experiments in neurons.DepositorInsertApex2
UseTagsVenus-CAAXExpressionMammalianMutationPromoterCAGAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMT-flp
Plasmid#67274PurposeConjugally transferable Flp recombinase plasmid for unmarked mutagenesisDepositorInserttetA, flp, repA101, oriR101
UseFlp recombinase plasmid, temperature sensitiveTagsHis-SUMO (flp)ExpressionMutationPromotertetA promoter of plasmid pACYC184 originAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
Influenza-FPV-HA-mEGFP
Plasmid#127810PurposeExpression vector Influenza A virus FPV (H7N1) hemagglutinin mEGFP fusion protein, plasma membrane localizationDepositorInsertInfluenza A virus FPV (H7N1) hemagglutinin mEGFP fusion
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGWB508-cTP-mCherry
Plasmid#213862PurposeBinary vector for the expression of mCherry flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)DepositorInsertPlastid transit peptide fused mCherry-UnaG
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only