Addgene

Showing: 1581 - 1600 of 28341 results

Results per page:

Sort results by:

plex_307-mKate-ccdB-g418

Plasmid

#201125

Purpose

pLEX_307 plasmid with N-terminal mKate tag and g418 marker

Depositor

Alejandro Chavez

Type

Empty backbone

Use

Tags

mKate

Expression

Mammalian

Mutation

Promoter

EF-1α

Available since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-PGK-NXPH4 (neo)

Plasmid

#192078

Purpose

Lentivirus for expression of NXPH4

Depositor

Roland Friedel

Insert

NXPH4 (NXPH4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hPGK

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_CMVTRE3G_LYSET-Isoform2-I142V-Flag

Plasmid

#192006

Purpose

Lentiviral vector to generate flag-tagged LYSET(TMEM251)-isoform2 I142V mutant stable expressing cell line under CMVTRE3G promoter

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-Isoform2-I142V-Flag (LYSET Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMVTRE3G

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_CMVTRE3G_LYSET-Isoform2-R45W-Flag

Plasmid

#192005

Purpose

Lentiviral vector to generate flag-tagged LYSET(TMEM251)-isoform2 R45W mutant stable expressing cell line under CMVTRE3G promoter

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-Isoform2-R45W (LYSET Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMVTRE3G

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-Hsp25 S15A/S86A

Plasmid

#187702

Purpose

Mutant Phenotypical Analysis

Depositor

Mary Beckerle

Insert

Hsp25 (Hspb1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

S15A/S86A

Promoter

CMV

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Cherry-Plekhh1 5 silent mutations

Plasmid

#187373

Purpose

Expresses human Plekhh1 with 5 silent mutations labelled with Cherry

Depositor

Evelyne Coudrier

Article

Prosperi et al J Cell Sci. 2021 Dec 1;134(23). pii: 272

Insert

Plekhh1 with 5 silent mutations

Use

Tags

mCherry

Expression

Mammalian

Mutation

Five silent mutations at the Plekhh1 siRNA site (…

Promoter

CMV

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-Plekhh1-MyTH-FERM

Plasmid

#187370

Purpose

Expresses human Plekhh1-MyTH-FERM labelled with EGFP

Depositor

Evelyne Coudrier

Article

Prosperi et al J Cell Sci. 2021 Dec 1;134(23). pii: 272

Insert

Plekhh1-MyTH-FERM

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
myr-palm-mEYFP-(L)-mApple

Plasmid

#182863

Purpose

Hetero-dimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

myr-palm-mEYFP-mApple

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
myr-palm-mCherry2-(L)-mApple

Plasmid

#182858

Purpose

Hetero-dimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

myr-palm-mCherry2-mApple

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
JAM2_pLENTI-CAG-IRES-GFP

Plasmid

#176997

Purpose

Mammalian lentiviral expression vector encoding JAM2

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

JAM2 (JAM2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CAG

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Rcan1_pLENTI-CAG-IRES-GFP

Plasmid

#177000

Purpose

Mammalian lentiviral expression vector encoding Rcan1

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

Rcan1 (Rcan1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CAG

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
RWDD2B_pLENTI-CAG-IRES-GFP

Plasmid

#177001

Purpose

Mammalian lentiviral expression vector encoding RWDD2B

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

RWDD2B (RWDD2B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CAG

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CRYZL1_pLENTI-CAG-IRES-GFP

Plasmid

#177004

Purpose

Mammalian lentiviral expression vector encoding CRYZL1

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

CRYZL1 (CRYZL1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CAG

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
ETS2_pLENTI-CAG-IRES-GFP

Plasmid

#177005

Purpose

Mammalian lentiviral expression vector encoding ETS2

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

ETS2 (ETS2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CAG

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti6.2-coHPDL H258A 3xFlag-V5

Plasmid

#174130

Purpose

Expresses codon-optimized catalytically inactive HPDL with a 3xFlag-V5 tag in mammalian cells

Depositor

Michael Pacold

Article

Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

Insert

4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)

Use

Lentiviral

Tags

3xFLAG-V5

Expression

Mammalian

Mutation

Codon optimized, H258A

Promoter

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-PGK-RAP2A-WT-puro

Plasmid

#156170

Purpose

Lentiviral vector for expression of RAP2A-WT, with puromycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

wild type form

Promoter

human PGK

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX305-N-dTAG-CARD8-ZUC

Plasmid

#169990

Purpose

Mammalian expression of the dTAG-CARD8 ZUC (L162M-L537) fusion protein for controllable activation with dTAG ligands (dTAG13, dTAGV-1).

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Tags

dTAG

Expression

Mammalian

Mutation

Promoter

hPGK

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pVD1 Multiplexing Edit E3-E4-En-1 (GB2244)

Plasmid

#160566

Purpose

GBoligomers for the position [3_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

Insert

Multiplexing Edit (E3-E4-En-1)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGB3 alpha2 OplexA:mini35S:PhiC31int:Tnos (GB1529)

Plasmid

#160614

Purpose

TU for the regulated expression of the PhiC31 phage integrase gene driven by a synthetic promoter consisting of the LexA operator fused to the minimal 35S promoter.

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

PhiC31

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

mini35s

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX304-ARF5-T161A-mNeonGreen

Plasmid

#162026

Purpose

Fast cycling ARF mutant

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

ARF5 (ARF5 Human)

Use

Lentiviral

Tags

Expression

Mutation

T161A

Promoter

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge