We narrowed to 4,850 results for: U6...

1. px330_SMAD exon 2 gRNA

   Plasmid

   #68350

   Purpose

   px330 with gRNA towards SMAD exon 2. Cas9 is expressed from a CAG promoter.

   Depositor

   Jannik Elverløv-Jakobsen

   Insert

   SMAD exon 2 gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. px330_P53 exon 8 gRNA

   Plasmid

   #68349

   Purpose

   px330 with gRNA towards P53 exon 8. Cas9 is expressed from a CAG promoter.

   Depositor

   Jannik Elverløv-Jakobsen

   Insert

   P53 exon 8 gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. px330_P53 exon 7 gRNA

   Plasmid

   #68351

   Purpose

   px330 with gRNA towards P53 exon 7. Cas9 is expressed from a CAG promoter.

   Depositor

   Jannik Elverløv-Jakobsen

   Insert

   P53 exon 7 gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. px330_PTEN exon 5 gRNA

   Plasmid

   #68348

   Purpose

   px330 with gRNA towards PTEN exon 5. Cas9 is expressed from a CAG promoter.

   Depositor

   Jannik Elverløv-Jakobsen

   Insert

   PTEN exon 5 gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. PasCas13b-gRNA-EGFP(W58stop)

   Plasmid

   #180507

   Purpose

   PasCas13b-gRNA targeting EGFP(W58stop) for RNA editing

   Depositor

   Masaharu Somiya

   Article

   Somiya et al bioRxiv 2022.01.25.477620

   Insert

   PasCas13b-gRNA targeting EGFP(W58stop)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. PasCas13b-gRNA-Nluc(W12stop)

   Plasmid

   #180506

   Purpose

   PasCas13b-gRNA targeting Nluc(W12stop) for RNA editing

   Depositor

   Masaharu Somiya

   Article

   Somiya et al bioRxiv 2022.01.25.477620

   Insert

   PasCas13b-gRNA targeting Nluc(W12stop)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV-gRNA-CTBP2-MGMT_1

   Plasmid

   #136419

   Purpose

   Lentiviral expression of gRNAs targeting intron 1 of human CTBP2 and intron 1 of human MGMT. Also constitutively expresses Puromycin fused to TagBFP.

   Depositor

   Massimo Squatrito

   Insert

   U6_sgRNA(CTBP2)_U6_sgRNA(MGMT)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKLV-gRNA-BTRC-MGMT_2

   Plasmid

   #136416

   Purpose

   Lentiviral expression of gRNAs targeting intron 2 of human BTRC and intron 1 of human MGMT. Also constitutively expresses Puromycin fused to TagBFP.

   Depositor

   Massimo Squatrito

   Insert

   U6_sgRNA(BTRC)_U6_sgRNA(MGMT)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6_empty-sgRNA(PP7)

   Plasmid

   #232432

   Purpose

   empty gRNA backbone which contains the PP7 aptamer in the scaffold tetraloop

   Depositor

   Gil Westmeyer

   Article

   Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

   Insert

   PP7-tagged sgRNA scaffold driven by human U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CMV-dCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-BGHpA

    Plasmid

    #171384

    Purpose

    Expression vector for encoding a human codon-optimized dCas13X.1-RESCUE-S driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

    Depositor

    Hui Yang

    Article

    Xu et al Nat Methods. 2021 May;18(5):499-506. doi

    Insert

    humanized dCas13X.1-RESCUE-S
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, U6

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV-minidCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-BGHpA

    Plasmid

    #171385

    Purpose

    Expression vector for encoding a human codon-optimized minidCas13X.1-RESCUE-S driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

    Depositor

    Hui Yang

    Article

    Xu et al Nat Methods. 2021 May;18(5):499-506. doi

    Insert

    humanized minidCas13X.1-RESCUE-S
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, U6

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6_rd6_pegRNA_(PP7-C4-Q1)

    Plasmid

    #232433

    Purpose

    pegRNA with optimized 3' modifications to correct the rd6 mutation

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    3'-PP7-tagged pegRNA for rd6 correction driven by human U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pU6_rd6_nsgRNA(PP7)

    Plasmid

    #232434

    Purpose

    nsgRNA for PE3b correction of the rd6 mutation, contains a PP7 in the scaffold tetraloop

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    PP7-tagged nicking gRNA (PE3b) for rd6 correction driven by human U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6_HEK3_nsgRNA(PP7)

    Plasmid

    #232444

    Purpose

    nsgRNA for PE3 facilitation of a +1 T to A prime edit on the HEK3 locus, contains a PP7 in the scaffold tetraloop

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    PP7-tagged nicking gRNA (PE3) for HEK3+1t>a edit driven by human U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pU6_rd12_pegRNA_(PP7-C4-Q1)

    Plasmid

    #232435

    Purpose

    pegRNA with optimized 3' modifications to correct the rd12 mutation

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    3'-PP7-tagged pegRNA for rd12 correction driven by human U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pU6_rd12_nsgRNA(PP7)

    Plasmid

    #232436

    Purpose

    nsgRNA for PE3b correction of the rd12 mutation, contains a PP7 in the scaffold tetraloop

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    PP7-tagged nicking gRNA (PE3b) for rd12 correction driven by human U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV KI Cloning Vector_hSyn mTagBFP2-CAAX2

    Plasmid

    #236245

    Purpose

    Cloning template for knock-in based strategy with U6 promoter, gRNA and donor tag cloning cassettes, and a fluorescent marker for the plasma membrane under hSyn promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    mTagBFP2 fused to the plasma membrane targeting sequence CAAX2
    

    Use

    AAV

    Tags

    mTagBFP2

    Expression

    Mutation

    Promoter

    U6 and human Synapsin1

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330.puro

    Plasmid

    #110403

    Purpose

    CRISPR/Cas9 vector with sgRNA expression and puromycin resistance

    Depositor

    Sandra Martha Gomes Dias

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO.1 LARP7-FLAG 7SK

    Plasmid

    #200733

    Purpose

    Lentiviral expression of Flag-Tagged human LARP7 and the snRNA 7SK

    Depositor

    Claudio Alarcón

    Insert

    LARP7 and 7SK (LARP7 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. px330_SMAD exon 9 gRNA

    Plasmid

    #68352

    Purpose

    px330 with gRNA towards SMAD exon 9. Cas9 is expressed from a CAG promoter.

    Depositor

    Jannik Elverløv-Jakobsen

    Insert

    SMAD exon 9 gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge