We narrowed to 1,640 results for: plasmid for e coli
-
-
GB1-ALIX(P801G)
Plasmid#180025PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(1-868, P801G). P801G mutation facilitates expression in E. coli.DepositorInsertGB1-His6-TEV-ALIX(1-868, P801G) (PDCD6IP Human)
UseTagsGB1-His6-TEVExpressionBacterialMutationP801GPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-optimizedNedd4.2d
Plasmid#165103Purposebacterial expression of a GST fusion of Human Nedd4.2dDepositorInsertNedd4.2d (NEDD4L Human)
UseTagsGST from plasmid.ExpressionBacterialMutationcodon optimized for E. coli expressionPromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-7
Plasmid#163135PurposepLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the C-IgAP surface expression anchor, nanobody, and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsNanobody, C-IgAP autotransporterExpressionBacterialMutationPromoterP(rhaB)Available sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCosSAD1AprR
Plasmid#190786PurposeAn E. coli cloning vector for the integration of heterologous genes into a linear plasmid pSA3239 in Streptomyces lavendulae subsp. lavendulae CCM 3239 through homologous recombinationDepositorInsertsupstream region of the aur1 cluster for auricin
ampramycin resistance cassette
downstream region of the aur1 cluster for auricin
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.9RT2-Pct5.1-crRNA(cadR)-RT(ΔcadR)
Plasmid#191647PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cadR-targeting spacer) cadR-deleting repair template,used for cadR deletionDepositorInsertCymR
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.1RT1-Pct5.1-crRNA(dadR)-RT(ΔdadR)
Plasmid#191635PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer) dadR-deleting repair template, used for dadR deletionDepositorInsertCymR
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSETa-mEos3.2-A69T
Plasmid#98571Purposephotoconvertible fluorescent protein mEos3.2-A69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coliDepositorInsertmEos3.2-A69T
UseTags7xHisExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.AD102
Plasmid#131403PurposeE. coli periplasmic expression of conformational nanobody Nb.AD102 recognizing purified active-state A2a adenosine receptorDepositorInsertNanobody AD102
UseTags6xHis-tag and pelB leaderExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSETa-mKikGR-V69T
Plasmid#98574Purposephotoconvertible fluorescent protein mKikGR-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coliDepositorInsertmKikGR-V69T
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLei-sfGFP-V2*D134*Y152*
Plasmid#191112PurposeAn E. coli sfGFP reporter with two TAG at positions 2 & 134 & 152DepositorInsertsuperfolder green fluorescence protein
UseTagsHis tagExpressionBacterialMutationchanging V2 & D134 & Y152 to TAGPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB1-PRD(703-868, P801G)
Plasmid#180029PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G). P801G mutation facilitates expression in E. coli.DepositorInsertGB1-His6-TEV-ALIX(703-868, P801G) (PDCD6IP Human)
UseTagsGB1-His6-TEVExpressionBacterialMutationP801GPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL1-MBP-DQNAT
Plasmid#198215PurposepJL1 plasmid encoding E. coli maltose binding protein (MBP) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tagDepositorInsertMBP_DQNAT
UseTags6x His Tag and DQNAT glycosylation tagExpressionBacterialMutationPromoterT7Available sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.6RT4-Pct5.1-crRNA(hcdR)-RT(ΔhcdR)
Plasmid#191646PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(hcdR-targeting spacer) hcdR-deleting repair template, used for hcdR deletionDepositorInsertCymR
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE-(NSP12)-pcI-ts,ind+-(NSP7-NSP8)
Plasmid#160540PurposeExpresses codon optimized SARS-CoV-2 NSP7, NSP8, and NSP12 in E. coli.DepositorInsertsUseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 10, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pExp-RBD-CHis
Plasmid#195002PurposeProduction of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tagDepositorInsertRBD (S SARS-CoV-2)
UseTagsHis8ExpressionBacterialMutationPromoterT7lacAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS-BsmbI-LoxP-Ef1a-EM7-mCherry-Neo-PolyA-LoxP
Plasmid#192822PurposeGolden Gate compatible plasmid with mCherry and NeoDepositorInsertmCherry and Neo
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-NBK-1
Plasmid#207639PurposeA plasmid for the expression of M. mazei pyrrolysine aminoacyl-tRNA synthetase/tRNA pair (PylRS/tRNAPyl) in E. Coli.DepositorInsertPyl-tRNACUA and 2x pyrrolysyl-tRNA synthetase (MmPylRS)
UseTagsExpressionBacterialMutationThe evolved synthetase NBK-1 contains mutations Y…PromoteraraBADAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.4RT4-Pct5.1-crRNA(cgr2)-RT(Δcgr12)
Plasmid#191650PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cgr2-targeting spacer) cgr1/cgr2-deleting repair template, used for cgr1/cgr2 deletionDepositorInsertCymR
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
MERS-coV-NSP3-c001
Plasmid#173085PurposeExpression of recombinant fusion protein in E. coliDepositorInsertrep (orf1ab Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (MERS-CoV))
UseTagsmCeruleanExpressionBacterialMutationD1109-D1275, codon optimizedPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only
