We narrowed to 10,377 results for: plasmids 101
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Plasmid#205247PurposeIn vitro transcription plasmid for production of dSpCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #63798.DepositorInsertdSpCas9-VPR
UseCRISPRAvailable SinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGDP1 NDM-1
Plasmid#112883PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGDP4 tet(A)
Plasmid#112887PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.DepositorInserttet(A)
Tags6xHisExpressionBacterialMutationSequence was codon optimized for E. coli K12 usin…PromoterPlacAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Dlx-FLEX-hM4Di-mCherry
Plasmid#209701PurposeExpresses hM4Di-mCherry under the control of distalless homeobox 5 and 6 (mDlx) enhancer in Cre-recombinase-dependent mannerDepositorInserthM4D(Gi)
UseAAVTagsmCherryAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 aph(3'')-Ia
Plasmid#112879PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXR004: CasRx pre-gRNA cloning backbone
Plasmid#109054PurposehU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRsDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRExpressionMammalianPromoterhU6Available SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV PIG Pu.1
Plasmid#66982PurposeRetroviral expression plasmid for mouse Pu.1 expression; containing bicistronic IRES-GFPDepositorAvailable SinceJuly 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDL17
Plasmid#106483PurposepDL17 is based on conditionally replicating backbone derived from pQE-30 and expresses gam, bet and exo genes under control of PrhaB promoter for efficient dsDNA integration into E. coli chromosomeDepositorInsertsgam
bet
exo
lacI
rhaR
rhaS
ExpressionBacterialAvailable SinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)
Plasmid#171129PurposeLwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)DepositorInsertU6 promoter entry plasmid for LwaCas13a crRNA cloning
UseCRISPRExpressionMammalianPromoterU6Available SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
p4B5-EGFP
Plasmid#140324PurposeDetection of Dengue virus infection in live cellsDepositorInsertDENV2-NGC NS4b5(10 a.a.)-NLS-eGFP
TagseGFPExpressionMammalianMutation2 conservative n.t. differences near 5' end …PromoterCMVAvailable SinceMay 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-NBL10
Plasmid#69971PurposeCre-conditionally expresses the anti-GFP Nanobody-Rpl10a fusionDepositorInsertNBL10
UseAAV and Cre/LoxTagsHAExpressionMammalianPromoterEF1aAvailable SinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTS2395-Tier1-PhCMV-Igk_mRuby2_KDEL
Plasmid#169533PurposeTier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 localized to the ER (PhCMV-Igk-mRuby2-KDEL-pA).DepositorInsertPCMV-driven ER-localized monomeric red fluorescent protein mRuby2
ExpressionMammalianPromoterPhCMVAvailable SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV Ef1a DIO GeNL_SS hGtACR1
Plasmid#233511PurposeLuciferase opsin fusion protein (luminopsin) for bioluminescent optogeneticsDepositorInsertsmNeonGreen
ISN-IE
hGtACR1
UseAAVTagsGaussia luciferase signal sequenceAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-hCYP26A1P-E4-mCherry
Plasmid#135478PurposeHuman short form promoter of human gene in pGL3-Basic-mCherry plasmid vector (Namely, E4.2)DepositorInsertmCherry Open reading Frame
UseUnspecifiedPromoterShort from promoter of human CYP26A1 (312 bp)Available SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTS1051-Tier1-PhCMV-4xCox8
Plasmid#169490PurposeTier-1 vector encoding PhCMV-driven four-fold repeat of a mitochondrial matrix localization signal derived from mitochondrial matrix protein Cox8 (PhCMV-4xCox8-pA)DepositorInsertPCMV-driven mitochondrial localization signal from cytochrome c oxidase subunit 8
ExpressionMammalianPromoterPhCMVAvailable SinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2394-Tier1-PhCMV-3xNLS_mTagBFP2
Plasmid#169531PurposeTier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 with nuclear localization (PhCMV-3xNLS-mTagBFP2-pA).DepositorInsertPCMV-driven nucleus-localized monomeric Tag blue fluorescent protein 2
ExpressionMammalianPromoterPhCMVAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mCherry.3xFLAG-WPRE
Plasmid#127861PurposepAAV plasmid expressing an mCherry.3xFLAG fusion protein under the hSyn promoterDepositorInsertmCherry
UseAAVTags3xFLAGPromoterhSynAvailable SinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVH268-Tier1-OTetO7-PCMVmin2-SEAP-p2A-iRFP670
Plasmid#169591PurposeTier-1 vector encoding PTetO7-driven SEAP-p2A-iRFP670 expression (OTetO7-PCMVmin-2-SEAP-p2A-iRFP670-pA).DepositorInserttetracycline-controlled SEAP and iRFP expression
ExpressionMammalianPromoterTetO7-PCMVmin-2Available SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1026-Tier1-PhCMV-3xNLS-iRFP670
Plasmid#169532PurposeTier-1 vector encoding PhCMV-driven iRFP670 that is localized to the nucleus (PhCMV-3xNLS-iRFP670-pA).DepositorInsertPCMV-driven nucleus-localized far-red fluorescent protein iRFP670
ExpressionMammalianPromoterPhCMVAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2381-Tier1-PhCMV-Igk_TM
Plasmid#169487PurposeTier-1 vector encoding PhCMV-driven transmembrane domain from Junctophilin-1 (TM) for membrane localization of fusion proteins attached to a secretion signal Igk (PhCMV-IgkSS-TM-pA).DepositorInsertPCMV-driven membrane localization
ExpressionMammalianPromoterPhCMVAvailable SinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only