161,407 results

1. pTRIP-CMV-mCherry-53BP1

   Plasmid

   #127658

   Purpose

   Over-express mCherry-53BP1 fragment to monitor 53BP1 foci.

   Depositor

   Nicolas Manel

   Article

   Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

   Insert

   53BP1 (TP53BP1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Nucleotides 3658-5133 of TP53BP1 (NM_005657.2) fu…

   Promoter

   Available since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOpen-GBDpol (exo)

   Plasmid

   #165572

   Purpose

   Robust and extremely thermostable polymerase with a half-life of 23 hours at 95 degrees C; offers 5x higher fidelity than Taq and robust performance. Ideal for GC-rich or looped sequences. Lacks exonuclease activity. Comparable to Deep Vent (exo-) DNA Polymerase at NEB

   Depositor

   FreeGenes Project, Drew Endy, Jennifer Molloy

   Insert

   Pyrococcus Sp. Heat-Stable (exo-) DNA Polymerase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pTF263 (pFA6a-TEV-6xGly-12xHis-His3MX)

   Plasmid

   #44069

   Depositor

   Tim Formosa

   Type

   Empty backbone

   Use

   Pcr-based yeast c-terminal tagging

   Tags

   12xHis tag, 6xGly linker, ADH1 transcription term…

   Expression

   Mutation

   Promoter

   Available since

   April 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

   Plasmid

   #60226

   Purpose

   Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Renilla luciferase
   Cre recombinase
   

   Use

   AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…

   Tags

   Cre-HA and Rluc-P2A

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and U6

   Available since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. p-dCas9-SSAP-MS2-BB_BbsI

   Plasmid

   #183826

   Purpose

   pU6-MS2-gRNA-backbone(BbsI)-CBH-dSpCas9-T2A-EBFP

   Depositor

   Le Cong

   Article

   Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

   Insert

   U6-guideRNA-dCas9-T2A-EBFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBH

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWZL hygro Flag HA TRAP220 wt

   Plasmid

   #17433

   Purpose

   Retroviral expression of Med1/TRAP220 in mammalian cells

   Depositor

   Kai Ge

   Article

   Ge et al Mol Cell Biol. 2008 Feb . 28(3):1081-91.

   Insert

   TRAP220 (MED1 Human)
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTRKH3-slpGFP

   Plasmid

   #27168

   Depositor

   Michela Lizier

   Article

   Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

   Insert

   surface (S)-layer protein promoter region fused with modified green fluorescent protein 5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+) Flag-His-ATM kd

   Plasmid

   #31986

   Depositor

   Michael Kastan

   Article

   Canman et al Science. 1998 Sep 11;281(5383):1677-9.

   Insert

   ATM kd (ATM Human)
   

   Use

   Tags

   Flag and His

   Expression

   Mammalian

   Mutation

   3'UTRf 9 nucl/D2870A N2875k. catalytically …

   Promoter

   Available since

   March 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT7TS-rich

   Plasmid

   #99050

   Purpose

   This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pT7TS. More sites for cloning are incorporated.

   Depositor

   Richard Bélanger

   Type

   Empty backbone

   Use

   Unspecified; Xenopus oocyte system

   Tags

   Expression

   Mutation

   Promoter

   pT7

   Available since

   Aug. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. PIK3C2GA

    Plasmid

    #42451

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PIK3C2GA (PIK3C2G Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    See Comments

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only