We narrowed to 5,913 results for: pcDNA 3.1
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Plasmid#78583PurposeExpresses GFP p6 with K27R mutation in mammalian cellsDepositorInserteGFP p6
UseTagsExpressionMammalianMutationHIV-1 HXB2 p6 with conservative mutations (G78A, …PromoterAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
RGS-6xHis-BLMP-1-pcDNA3.1-
Plasmid#52514Purposeexpresses C. elegans BLMP-1 in mammalian cells with RGS-6xHis tag at N-terminusDepositorInsertblmp-1 (blmp-1 Nematode)
UseTags6xHisExpressionMammalianMutationN75S mutation compared GenBank reference sequence…PromoterCMVAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
Human NCKX4-AS-short (pcDNA3.1+)
Plasmid#75210PurposeMammalian expression of SLC24A4DepositorInsertSLC24A4 (SLC24A4 Human)
UseTagsExpressionMammalianMutationalternatively spliced exon absentPromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
RGS-6xHis-DRE-1-pcDNA3.1-
Plasmid#52517Purposeexpresses C. elegans DRE-1 in mammalian cells with RGS-6xHis tag at N-terminusDepositorInsertDre-1 (dre-1 Nematode)
UseTagsRGS-6xHisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Nup88 (551-741)
Plasmid#64252PurposeMammalian expression of C-terminal domain of Nup88DepositorInsertNup88 (NUP88 Human)
UseTagshis and mycExpressionMammalianMutationC-terminal domain (551-741)PromoterCMVAvailable sinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-nAChr-beta3-CFP
Plasmid#50485Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorInsertnAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
UseTagsCFPExpressionMammalianMutationCFP fusion in M3-M4 loop (after residue P379). O…PromoterCMVAvailable sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HaloCKAR2.2-T2A-EGFP
Plasmid#216493PurposeExpression of chemigenetic PKC biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cellsDepositorInsertHaloCKAR2.2
UseTagsT2A-EGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HaloAktKAR2.2-T2A-EGFP
Plasmid#216494PurposeExpression of chemigenetic Akt biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cellsDepositorInsertHaloAktKAR2.2
UseTagsT2A-EGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HaloEKAR2.2-T2A-EGFP
Plasmid#216495PurposeExpression of chemigenetic ERK biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cellsDepositorInsertHaloEKAR2.2
UseTagsT2A-EGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HaloAKAR2.0-T2A-EGFP
Plasmid#216488PurposeExpression of chemigenetic PKA biosensor (high basal brightness, medium dynamic range) and EGFP transfection marker in mammalian cellsDepositorInsertHaloAKAR2.0
UseTagsT2A-EGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HaloAKAR2.1-T2A-EGFP
Plasmid#216489PurposeExpression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) and EGFP transfection marker in mammalian cellsDepositorInsertHaloAKAR2.1
UseTagsT2A-EGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HaloAKAR2.2-T2A-EGFP
Plasmid#216490PurposeExpression of chemigenetic PKA biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cellsDepositorInsertHaloAKAR2.2
UseTagsT2A-EGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-IARS2-H112A/P115A
Plasmid#232338PurposeExpresses human IARS2 with putative isoleucine binding deficiency mutations (H112A and P115A) in mammalian cells, also carrying synonymous mutations described in Addgene #232340DepositorInsertIARS2 (IARS2 Human)
UseTagsExpressionMammalianMutationPutative isoleucine binding deficiency mutation H…PromoterCMVAvailable sinceJune 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-IARS2-K664A/K667A
Plasmid#232339PurposeExpresses human IARS2 with putative tRNA binding deficiency mutations (K664A and K667A) in mammalian cells, also carrying synonymous mutations described in Addgene #232340DepositorInsertIARS2 (IARS2 Human)
UseTagsExpressionMammalianMutationPutative tRNA binding deficiency mutation K664A A…PromoterCMVAvailable sinceJune 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SARS-CoV-2-S-ectodomain
Plasmid#235984PurposeInternally tagged SARS-CoV-2 spike ecto-domainDepositorInsertSpike protein (S SARS-CoV-2)
UseTags8xHis and Twin-Strep tag and internal Twin-Strep …ExpressionMammalianMutationaa 1-1208, D614G, RRAR684-684GSAS, and DK987-987P…PromoterCMVAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SARS2-Spike-HiBiT
Plasmid#191212PurposeTransient expression in mammalian cells of SARS-CoV-2 (A1) spike protein tagged on the C-terminus with HiBiT, the high affinity small fragment of split nanoluciferase assayDepositorInsertSARS-CoV-2 spike_WT (S SARS CoV-2)
UseTagsHiBiT (high affinity small nanoluciferase fragmen…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-DDR1-F866Y-V5/HIS
Plasmid#236009Purposeexpression of the F866Y kinase defective mutant variant of human DDR1 that might be associated with lung AdenocarcinomaDepositorInserthuman DDR1-F866Y receptor tyrosine kinase, full length (DDR1 Human)
UseTagsV5/HisExpressionMammalianMutationF866Y substitutionPromoterCMVAvailable sinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PDGFRA-V561D-V5/HIS
Plasmid#234762PurposeExpression of the V561D mutant variant of human PDGFRA, which is associated with Gastrointestinal Stromal Tumor and GIST-Plus Syndrome, , constantly activatedDepositorInsertPDGFRA-V561D receptor tyrosine kinase, full length (PDGFRA Human)
UseTagsV5/HisExpressionMammalianMutationV561D substitutionPromoterCMVAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PDGFRA-P577S-V5/HIS
Plasmid#234760PurposeExpression of the P577S mutant variant of human PDGFRA, which might be associated with MelanomaDepositorInsertPDGFRA-P577S receptor tyrosine kinase, full length (PDGFRA Human)
UseTagsV5/HisExpressionMammalianMutationP577S substitutionPromoterCMVAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PDGFRA-R487L-V5/HIS
Plasmid#234761PurposeExpression of the R487L mutant variant of human PDGFRA, which might be associated with Gastrointestinal Stromal TumorDepositorInsertPDGFRA-R487L receptor tyrosine kinase, full length (PDGFRA Human)
UseTagsV5/HisExpressionMammalianMutationR487L substitutionPromoterCMVAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only