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Showing: 2401 - 2430 of 22251 results

  7. SSTR3-DuET

    Plasmid
    #213378
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    SSTR3 (SSTR3 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213378-plasmid-map-sequence-id-430734

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  9. pET-scFv-T

    Plasmid
    #67843
    Purpose
    pET-30-based vector dedicated to efficient scFv expression, which circumvents the problem of in-frame amber codons in scFvs.
    Depositor
    Joanna Bereta
    Article
    Ossysek et al Immunol Lett. 2015 Jul 25. pii: S0165-24
    Insert
    antibody 1D8 light kappa chain
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    67843-plasmid-map-sequence-id-117725

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  13. pRedCmOcr

    Plasmid
    #176226
    Purpose
    A helper plasmid for expression of the artificial T7ocr-gam-bet-exo operon. The plasmid carries the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage.
    Depositor
    Sergey Sineoky
    Article
    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89
    Insert
    Lambda Red-T7ocr operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PrhaB and PL
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    176226-plasmid-map-sequence-id-347002

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  17. pBA-mKeima-PIM

    Plasmid
    #174650
    Purpose
    Low level expression of mKeima-PIM. Can be used for ratiometric detection of inducible aggregate acidification after addition of AP20187. mKeima fluorophore allows for simultaneous GFP-tagged imaging.
    Depositor
    Lukas Kapitein
    Article
    Janssen et al J Cell Sci. 2021 Oct 1;134(19). pii: 271
    Insert
    xXFKBP homo-mKeima-2x FKBP homo-2x FKBP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Val24Glu, Tyr80Cys, Ala95Thr mutations in second …
    Promoter
    Chicken beta-actin
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    174650-plasmid-map-sequence-id-346673

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  19. NTSR1-DuET

    Plasmid
    #213356
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    NTSR1 (NTSR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213356-plasmid-map-sequence-id-430917

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  21. SSTR2-DuET

    Plasmid
    #213377
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    SSTR2 (SSTR2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213377-plasmid-map-sequence-id-430733

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  24. eSpCas9(1.1)_No_FLAG_NPM1_G6

    Plasmid
    #178091
    Purpose
    Expresses the NPM1 G6 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with NPM1_mNeonGreen_Donor to tag NPM1 with mNeonGreen. pX330-like plasmid.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    NPM1 G6 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 promoter
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178091-plasmid-map-sequence-id-350141

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  29. pJJR83

    Plasmid
    #75028
    Purpose
    mCherry^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Mike Boxem
    Article
    Ramalho et al Development. 2020 Jul 22;147(14):dev1880
    Insert
    mCherry^SEC^3xFlag
    Use
    CRISPR
    Tags
    3xFLAG and C. elegans codon optimized mCherry
    Expression
    Worm
    Mutation
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75028-plasmid-map-sequence-id-141918

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Showing: 2401 - 2430 of 22251 results