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pCS2_RFP_2xRecEnhSTOP_EcoRI_EYFP

Plasmid

#250547

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a flourenscent reporter containing an EcoRI site with two editible stop codons

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

RFP_2xRecEnhSTOP_EcoRI_EYFP

Use

Synthetic Biology

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_RFP_1xRecEnhSTOP_EcoRI_EYFP

Plasmid

#250548

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a flourenscent reporter containing an EcoRI site with one editible stop codon

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

RFP_1xRecEnhSTOP_EcoRI_EYFP

Use

Synthetic Biology

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_RFP_2xRecEnhancedSTOP_1xBoxB_EYFP

Plasmid

#250545

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a flourenscent reporter containing a box-b hairpin with two editible stop codon

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

RFP_2xRecEnhancedSTOP_1xBoxB_EYFP

Use

Synthetic Biology

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_drePum1_HA_dreAdar1

Plasmid

#250541

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of zebrafish Pum1 fused to the catalytic domain of zebrafish Adar1

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

drePum1_HA_dreAdar1

Use

Synthetic Biology

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_EYFP_3xBoxB_Recorder_SV40

Plasmid

#250534

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a recorder containing 3 box-b hairpins

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

EYFP_3xBoxB_Recorder_SV40

Use

Synthetic Biology

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_RFP_1xRecEnhancedSTOP_1xBoxB_EYFP

Plasmid

#250543

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a flourenscent reporter containing a box-b hairpin with one editible stop codon

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

RFP_1xRecEnhancedSTOP_1xBoxB_EYFP

Use

Synthetic Biology

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_RFP_1xRecEnhancedEDITED_1xBoxB_EYFP

Plasmid

#250544

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of a flourenscent reporter containing a box-b hairpin without an editible stop codon

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

RFP_1xRecEnhancedEDITED_1xBoxB_EYFP

Use

Synthetic Biology

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_N22_HA_dreAdar1_E488Q

Plasmid

#250533

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of the catalytic domain of zerbafish Adar1 (E488Q)

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

N22_HA_dreAdar1_E488Q

Use

Synthetic Biology

Mutation

E488Q

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_N22_HA_dreAdar1

Plasmid

#250532

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of N22 peptide fused to the catalytic domain on zebrafish Adar1

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

N22_HA_dreAdar1

Use

Synthetic Biology

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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DiapAR10

Plasmid

#245843

Purpose

Archael (Diapherotrites AR10) 16S rRNA expression

Depositor

Roland Hatzenpichler

Article

Van Beek et al Microbiol Resour Announc. 2025 Dec 11;14

Insert

16S rRNA

Use

Other

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS-V5-Scip

Plasmid

#225110

Purpose

Scip expression in Chick

Depositor

Polyxeni Philippidou

Article

Kc et al Commun Biol. 2024 Sep 17;7(1):1166. doi:

Insert

Scip (Pou3f1 Mouse)

Use

Avian expression

Mutation

WT

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pGEM-Isce-Tol2-Tn5-neo

Plasmid

#176619

Purpose

for generating PCR templates with Tol2 repeats and Isce sites for BAC recombination

Depositor

Nathan Lawson

Article

Shih et al Development. 2021 Nov 9. pii: 273393. do

Insert

I-SceI sites, Tol2 repeats and Tn5-neo cassette

Use

Unspecified

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pX330A_FokI-1x5

Plasmid

#63605

Purpose

Expresses FokI-dCas9 and gRNA

Depositor

Takashi Yamamoto

Article

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Insert

FokI-dCas9

Use

CRISPR

Expression

Mammalian

Promoter

CBh

Available Since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Rem2 RNAiR2 short

Plasmid

#51596

Purpose

expresses RNAiR myc-tagged rat Rem2 (short version, missing 69 AA at N terminus)

Depositor

Suzanne Paradis

Article

Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

Insert

Rem2 (Rem2 Rat)

Tags

myc

Expression

Mammalian

Mutation

silent mutations at shRNA 1 and 2 recognition sit…

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-myc-Rem2 R236A/L263A

Plasmid

#51589

Purpose

expresses RNAiR myc-tagged Rem2 non-Ca channel interacting

Depositor

Suzanne Paradis

Article

Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

Insert

Rem2 (Rem2 Mouse)

Tags

myc

Expression

Mammalian

Mutation

R236A and L263A; silent mutations at shRNA 1 and …

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Rem2 S69A/S334A

Plasmid

#51588

Purpose

expresses RNAiR myc-tagged Rem2 non-14-3-3 interacting

Depositor

Suzanne Paradis

Article

Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

Insert

Rem2 (Rem2 Mouse)

Tags

myc

Expression

Mammalian

Mutation

S69A and S334A; silent mutations at shRNA 1 and 2…

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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Sup35 Sc/Ca NM Chimera S17R His7 Cys

Plasmid

#15601

Depositor

Susan Lindquist

Article

Tessier et al Nature. 2007 May 31. 447(7144):556-61.

Insert

Sup35 NM (SUP35 Budding Yeast, Candida albicans)

Tags

7xHis and Cysteine

Expression

Bacterial

Mutation

S17R. Serine 17 mutated to arginine.

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

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WT Ecm2

Plasmid

#169922

Purpose

Ecm2 +/-300bp of up- and downstream seq. cloned into the NotI and SalI sites of pRS416

Depositor

Aaron Hoskins

Article

van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

Insert

Ecm2

Expression

Bacterial and Yeast

Availability

Academic Institutions and Nonprofits only

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MSCV-EGFP

Plasmid

#91975

Purpose

EGFP reporter cell line control (no miR-19 sites)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

EGFP

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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nontarget S. pyogenes scaffold CARGO array

Plasmid

#191319

Purpose

Contains an array of nontargeting guide RNAs

Depositor

Joanna Wysocka

Article

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Insert

Array of guide RNAs

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pMTS-hFluc

Plasmid

#177716

Purpose

Firefly luciferase reporter fused to a mitochondria signal sequence for mitochondrial targeting

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

hFluc

Tags

Mitochondrial Targeting Sequence MTS SS

Expression

Bacterial, Mammalian, and Y…

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pTrcHisA-rat-PTP1B

Plasmid

#35987

Depositor

Anna Huttenlocher

Article

Cortesio et al J Cell Biol. 2008 Mar 10;180(5):957-71.

Insert

protein tyrosine phosphatase 1B (Ptpn1 Rat)

Tags

His and Xpress Epitope Tag

Expression

Bacterial

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

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MSCV-IRES-Thy1.1 DEST

Plasmid

#17442

Purpose

Destination vector derived from MSCV-IRES-Thy1.1. Please note that this plasmid contains attB1 and attB2 sites for use in a BP reaction

Depositor

Anjana Rao

Article

Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only

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PiggyBac dCas9-VPR

Plasmid

#191269

Purpose

piggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFP

Depositor

Joanna Wysocka

Article

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Inserts

VPR-dCas9-HA-tagBFP
rtTA3-IRES-PURO

Use

CRISPR

Tags

HA and tagBFP

Expression

Mammalian

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pSV Sport SREBP-1c dom neg

Plasmid

#8885

Depositor

Bruce Spiegelman

Article

Kim et al Genes Dev. 1996 May 1. 10(9):1096-107.

Insert

SREBP-1c (Srebf1 Rat)

Expression

Mammalian

Mutation

Y305A amino acid substitution in the active versi…

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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PiggyBac dCas9-KRAB

Plasmid

#191314

Purpose

piggyBac integration and dox-inducible expression of KRAB-dCas9-HA-tagBFP

Depositor

Joanna Wysocka

Article

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Inserts

KRAB-dCas9-HA-tagBFP
rtTA3-IRES-PURO

Use

CRISPR

Tags

HA and tagBFP

Expression

Mammalian

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pSCALPS_miR-146aSponge_mCherry_GFP

Plasmid

#149718

Purpose

Lentiviral vector expressing a miR-146a decoy sponge

Depositor

Silvia Monticelli

Article

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

14X miR-146a binding sites

Use

Lentiviral

Expression

Mammalian

Mutation

G to T mutation in position 75 of the insert

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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Galt1-HT7-mGold

Plasmid

#230027

Purpose

Expressing fluorescent HaloTag7 fusion protein in mammalian cells with localization in the Golgi apparatus.

Depositor

Pablo Rivera-Fuentes

Insert

Galt1-HaloTag7-mGold (B4GALT1 Human, Synthetic)

Tags

HaloTag,mGold

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Galt1-Grx1-roGFP1-iE

Plasmid

#230029

Purpose

Expressing Grx1-roGFP1-iE fluorescent fusion protein in mammalian cells with localization in the Golgi apparatus.

Depositor

Pablo Rivera-Fuentes

Insert

Galt1-Grx1-roGFP1-iE (B4GALT1 Human, Synthetic)

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Galt1-sCGrx1p-HA

Plasmid

#230028

Purpose

Expressing sCGrx1p protein fused to HA tag in mammalian cells with localization in the Golgi apparatus.

Depositor

Pablo Rivera-Fuentes

Insert

Galt1-sCGrx1p-HA (B4GALT1 Human, Synthetic)

Tags

HA

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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