Addgene

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pIS1 IMP-1 long-mut-let-7

Plasmid

#21642

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

IGF-II mRNA-binding protein 1 (IGF2BP1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant let-7 microRNA binding sites

Promoter

Available since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pENTR-AtmiR173aTS-B/c

Plasmid

#227964

Purpose

Entry plasmid with AtmiR173aTS and a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acid Research 2025

Inserts

B/c
AtmiR173aTS

Use

Tags

Expression

Mutation

Promoter

None

Available since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pMDC32B-AtmiR173aTS-B/c

Plasmid

#227965

Purpose

Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. For expressing syn-tasiRNAs in Arabidopsis thaliana.

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acid Research 2025

Inserts

B/c
AtmiR173aTS

Use

Tags

Expression

Plant

Mutation

Promoter

35S and None

Available since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-NbmiR482aTS-B/c

Plasmid

#227966

Purpose

Entry plasmid with NbmiR482aTS and a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acid Research 2025

Inserts

B/c
NbmiR482aTS

Use

Tags

Expression

Mutation

Promoter

None

Available since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pMDC32B-NbmiR482aTS-B/c

Plasmid

#227967

Purpose

Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. For expressing syn-tasiRNAs in Nicotiana benthamiana.

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acid Research 2025

Inserts

B/c
NbmiR482aTS

Use

Tags

Expression

Plant

Mutation

Promoter

35S and None

Available since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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A1-LCD -3D+3N

Plasmid

#234614

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 3 aspartates mutated to asparagine

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_3DN (HNRNPA1 Human)

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

D214N, D242N, D250N

Promoter

T7

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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A1-LCD -4D+4N

Plasmid

#234611

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 4 aspartates mutated to asparagine

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_4DN (HNRNPA1 Human)

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

D214N, D242N, D250N, D262N

Promoter

T7

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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A1-LCD -4D+4V

Plasmid

#234610

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 4 aspartates mutated to valine

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_4DV (HNRNPA1 Human)

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

D214V, D242V, D250V, D262V

Promoter

T7

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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A1-LCD -3D+3V

Plasmid

#234613

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 3 aspartates mutated to valine

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_3DV (HNRNPA1 Human)

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

D214V, D242V, D250V

Promoter

T7

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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fabp10a:lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR; cryaa:mCherry

Plasmid

#230043

Purpose

Partial ablation and subsequent lineage tracing of regenerating hepatocytes in zebrafish.

Depositor

Sumeet Pal Singh

Insert

lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR

Use

Cre/Lox; Zebrafish expression

Tags

Expression

Mutation

Promoter

fabp10a

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRC1 CTFdeltaTA-FLAG

Plasmid

#217717

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

CELSR1 (CELSR1 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-2453

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-MBP-TEVpCS-ADGRB2 CTFdeltaTA-FLAG

Plasmid

#217715

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRB2 (BAI2 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-917

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-MBP-TEVpCS-ADGRA3 CTFdeltaTA-FLAG

Plasmid

#217713

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRA3 (ADGRA3 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-743

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-MBP-TEVpCS-ADGRV1 CTFdeltaTA-FLAG

Plasmid

#217743

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRV1 (ADGRV1 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-5896

Promoter

Available since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-MBP-TEVpCS-ADGRL4 CTFdeltaTA-FLAG

Plasmid

#217742

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRL4 (ADGRL4 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-412

Promoter

Available since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-MBP-TEVpCS-ADGRB3 CTFdeltaTA-FLAG

Plasmid

#217716

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRB3 (BAI3 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-862

Promoter

Available since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-MBP-TEVpCS-ADGRG4 CTF-FLAG

Plasmid

#217702

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRG4 (ADGRG4 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-2721

Promoter

Available since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-MBP-TEVpCS-ADGRD1 CTF-FLAG

Plasmid

#217687

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRD1 (ADGRD1 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-544

Promoter

Available since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-MBP-TEVpCS-ADGRC3 CTF-FLAG

Plasmid

#217686

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

CELSR3 (CELSR3 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-2516

Promoter

Available since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-MBP-TEVpCS-ADGRC2 CTF-FLAG

Plasmid

#217685

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

CELSR2 (CELSR2 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-2356

Promoter

Available since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-MBP-TEVpCS-ADGRC1 CTF-FLAG

Plasmid

#217684

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

CELSR1 (CELSR1 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-2447

Promoter

Available since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-MBP-TEVpCS-ADGRG1 CTFdeltaTA-FLAG

Plasmid

#217732

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRG1 (ADGRG1 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-388

Promoter

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-MBP-TEVpCS-ADGRC3 CTFdeltaTA-FLAG

Plasmid

#217719

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

CELSR3 (CELSR3 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-2522

Promoter

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-MBP-TEVpCS-ADGRB3 CTF-FLAG

Plasmid

#217683

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRB3 (BAI3 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-856

Promoter

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-MBP-TEVpCS-ADGRV1 CTF-FLAG

Plasmid

#217710

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRV1 (ADGRV1 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-5890

Promoter

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-MBP-TEVpCS-ADGRA2 CTFdeltaTA-FLAG

Plasmid

#217712

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRA2 (ADGRA2 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-752

Promoter

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-MBP-TEVpCS-ADGRC2 CTFdeltaTA-FLAG

Plasmid

#217718

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

CELSR2 (CELSR2 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-2362

Promoter

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-MBP-TEVpCS-ADGRD1 CTFdeltaTA-FLAG

Plasmid

#217720

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRD1 (ADGRD1 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-550

Promoter

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-MBP-TEVpCS-ADGRG1 CTF-FLAG

Plasmid

#217699

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRG1 (ADGRG1 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-382

Promoter

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-MBP-TEVpCS-ADGRG2 CTF-FLAG

Plasmid

#217700

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRG2 (ADGRG2 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-606

Promoter

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 2401 - 2430 of 20870 results

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