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We narrowed to 18,759 results for: nls

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Showing: 3201 - 3240 of 18759 results

  2. CMPE-ePPEplus

    Plasmid
    #205242
    Purpose
    For multiplex plant prime editing in wheat plants or monocotyledons protoplasts
    Depositor
    Zong Yuan
    Publication
    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:
    Insert
    Csy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Detailed in manuscript
    Available since
    Oct. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    205242-plasmid-map-sequence-id-408832

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  4. pCM61

    Plasmid
    #261133
    Purpose
    For N-terminal tagging a protein of interest with VN173 (split-YFP) and mTagBFP expression. No promoter. Can be integrated at single-copy into genome using RMCE.
    Depositor
    John Calarco
    Publication
    Martin et al bioRxiv 2025.02.28.640866
    Insert
    AID::nls::mTagbfp2::2a::AID::VN173::tbb-2 3' in pLF3FShC
    Expression
    Worm
    Promoter
    none
    Available since
    Sept. 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    261133-plasmid-map-sequence-id-520987

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  5. pCM60

    Plasmid
    #261132
    Purpose
    For N-terminal tagging a protein of interest with VC155 (split-YFP) and mCherry expression. No promoter. Can be integrated at single-copy into genome using RMCE.
    Depositor
    John Calarco
    Publication
    Martin et al bioRxiv 2025.02.28.640866
    Insert
    AID::nls::mcherry::2a::AID::VC155::tbb-2 3' in pLF3FShC
    Expression
    Worm
    Promoter
    none
    Available since
    Sept. 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    261132-plasmid-map-sequence-id-520976

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  6. pCM62

    Plasmid
    #261130
    Purpose
    For C-terminal tagging a protein of interest with VC155 (split-YFP) and mCherry expression. No promoter. Can be integrated at single-copy into genome using RMCE.
    Depositor
    John Calarco
    Publication
    Martin et al bioRxiv 2025.02.28.640866
    Insert
    AID::nls::mcherry::2a::AID::VC155::tbb-2 3' in pLF3FShC
    Expression
    Worm
    Promoter
    none
    Available since
    Sept. 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    261130-plasmid-map-sequence-id-520970

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  7. pCM63

    Plasmid
    #261131
    Purpose
    For C-terminal tagging a protein of interest with VN173 (split-YFP) and mTagBFP expression. No promoter. Can be integrated at single-copy into genome using RMCE.
    Depositor
    John Calarco
    Publication
    Martin et al bioRxiv 2025.02.28.640866
    Insert
    AID::nls::mTagbfp2::2a::AID::VN173::tbb-2 3' in pLF3FShC
    Expression
    Worm
    Promoter
    none
    Available since
    Aug. 31, 2026
    Availability
    Academic Institutions and Nonprofits only
    261131-plasmid-map-sequence-id-520102

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  8. pEB2_4_CusR-VIII

    Plasmid
    #236219
    Purpose
    CusR-based expression system: Plasmid with cusR-mTurquoise2-VP64-SV40 under RET2p and mCherry under 8xCusR_BSsENO1cp with homologous regions for genome integration into HO locus.
    Depositor
    Yvonne Nygård
    Publication
    Blick et al Synth Biol (Oxf). 2025 Dec 4;10(1):ysaf0
    Inserts
    cusR
    mCherry, SSA1 terminator
    Use
    Synthetic Biology
    Tags
    mTurquoise2-VP64-NLS
    Expression
    Bacterial
    Promoter
    8xCusR_BSsENO1cp and RET2p
    Available since
    Aug. 18, 2026
    Availability
    Academic Institutions and Nonprofits only
    236219-plasmid-map-sequence-id-519035

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  9. GLuc VLP

    Plasmid
    #182213
    Purpose
    YIp for expressing MPyV deltaVP1 (GAL1 promoter) and Gaussia luciferase tagged with VP2C (GAL10 promoter).
    Depositor
    Claudia Vickers
    Inserts
    VP2C-GLuc
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    GAL1 and GAL10
    Available since
    March 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    182213-plasmid-map-sequence-id-357719

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  10. Akaluc VLP

    Plasmid
    #182212
    Purpose
    YIp for expressing MPyV deltaVP1 (GAL1 promoter) and Akaluc tagged with VP2C (GAL10 promoter).
    Depositor
    Claudia Vickers
    Inserts
    VP2C-Akaluc
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    Use
    Synthetic Biology; Tem imaging
    Expression
    Yeast
    Promoter
    GAL1 and GAL10
    Available since
    March 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    182212-plasmid-map-sequence-id-357717

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  14. JPF0515

    Plasmid
    #124006
    Purpose
    Encodes pCMV-C driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Publication
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pCMV-C-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    Expression
    Mammalian
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124006-plasmid-map-sequence-id-252389

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  15. JPF0516

    Plasmid
    #124007
    Purpose
    Encodes pCMV-D driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Publication
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pCMV-D-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    Expression
    Mammalian
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124007-plasmid-map-sequence-id-252391

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  16. JPF0517

    Plasmid
    #124008
    Purpose
    Encodes pCMV-E driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Publication
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pCMV-E-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    Expression
    Mammalian
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124008-plasmid-map-sequence-id-252393

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  17. JPF0518

    Plasmid
    #124009
    Purpose
    Encodes pCMV-F driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Publication
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pCMV-F-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    Expression
    Mammalian
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124009-plasmid-map-sequence-id-252413

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  18. JPF0519

    Plasmid
    #124010
    Purpose
    Encodes pCMV-G driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Publication
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pCMV-G-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    Expression
    Mammalian
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124010-plasmid-map-sequence-id-252415

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  20. 3xFN-Tel-TAL/pMXs-neo

    Plasmid
    #63591
    Purpose
    Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat. A retroviral expression vector with the neomycin resistance gene.
    Depositor
    Hodaka Fujii
    Publication
    Fujita et al Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038
    Insert
    3xFVN-Tel-TAL
    Use
    Retroviral and TALEN
    Tags
    3xFLAG-tag, V5-tag, and nuclear localization sign…
    Expression
    Mammalian
    Promoter
    LTR
    Available since
    April 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    63591-plasmid-map-sequence-id-108371

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  23. pCMV-T7-nSpCas9(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES24)

    Plasmid
    #185492
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpCas9(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Publication
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpCas9(D10A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    CMV and T7
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185492-plasmid-map-sequence-id-363405

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  24. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-gs-UGI-NLS(SV40)(LbBE1.4) (RTW1293)

    Plasmid
    #114086
    Purpose
    CAG promoter expression plasmid for human codon optimized LbBE1.4
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.4)
    Tags
    2x SV40 NLS and SV40 NLS
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114086-plasmid-map-sequence-id-231034

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  27. 5aa SunTag VP64 EVD

    Plasmid
    #115483
    Purpose
    CRISPR-Cas9 SunTag system to target VP64 to the EVD/ATR loci each with two guide RNAs
    Depositor
    Steven Jacobsen
    Publication
    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:
    Insert
    g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    March 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    115483-plasmid-map-sequence-id-238821

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  29. pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALentry

    Plasmid
    #69233
    Purpose
    R1335K mutant SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)
    Depositor
    Scot Wolfe
    Publication
    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n
    Insert
    SpCas9
    Use
    CRISPR
    Tags
    2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…
    Expression
    Mammalian
    Mutation
    Arginine 1335 is changed to Lysine
    Available since
    Nov. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    69233-plasmid-map-sequence-id-122312

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  31. AAV2 ITR_phU6_gRNA scaffold-DMD sg1_phU6_gRNA scaffold-DMD sg16_pMHCK7-3x Flag-NLS-enOsCas12f1-NLS_pA_AAV2 ITR

    Plasmid
    #197028
    Purpose
    AAV vector encoding a human codon-optimized enOsCas12f1 driven by MHCK7 promoter, DMD-targeting guide RNAs compatible with enOsCas12f1 driven by hU6
    Depositor
    Xiangfeng Kong
    Publication
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Insert
    humanized enOsCas12f1
    Use
    AAV
    Mutation
    D52R / T132R
    Promoter
    MHCK7, hU6
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197028-plasmid-map-sequence-id-388968

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  36. AAV-SYN-NLS-SaCas9-NLS-3xHA-bGHpA-U6-BsaI-sgRNA

    Plasmid
    #218710
    Purpose
    AAV vector plasmid expressing Cas9 from Staphylococcus aureus (SaCas9) under the human synapsin (SYN) promoter and its sgRNA under the U6 promoter
    Depositor
    Bart Nieuwenhuis
    Inserts
    hSaCas9
    Chimeric guide for SaCas9
    Use
    AAV and CRISPR
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218710-plasmid-map-sequence-id-433357

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  39. pL6puro-JeT-pmRevErbA-miRFP713-NLS-Pest

    Plasmid
    #240110
    Purpose
    A lentiviral (infrared) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by synthtic JeT promoter.
    Depositor
    Achim Kramer
    Publication
    Gabriel et al bioRxiv 2025.06.30.661926
    Insert
    murine Nr1d1 promoter+miRFP713-NLS-Pest-Reporter (Nr1d1 Mouse)
    Use
    Lentiviral
    Mutation
    added JeT Promoter
    Available since
    Sept. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    240110-plasmid-map-sequence-id-485468

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  40. pL6puro-pSV40(3)-pmRevErbA-Venus-NLS-Pest

    Plasmid
    #240114
    Purpose
    A lentiviral (yellow) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by SV40 promoter fragment.
    Depositor
    Achim Kramer
    Publication
    Gabriel et al bioRxiv 2025.06.30.661926
    Insert
    murine Nr1d1 promoter+Venus-NLS-Pest-Reporter (Nr1d1 Mouse)
    Use
    Lentiviral
    Mutation
    added SV40-Enhancer
    Available since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    240114-plasmid-map-sequence-id-481774

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Showing: 3201 - 3240 of 18759 results