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Plasmid#182365PurposeExpresses Neq2X DNA polymeraseDepositorInsertNeq2X
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR-L1-PGK-TKNeo-L2
Plasmid#141008PurposeMutisite Gateway mediated vector constructionDepositorInsertTKNeo
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTK295
Plasmid#59564PurposeSIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak EGFPDepositorInsertSGP(14) 2xK-turn Kozak EGFP
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGNW4
Plasmid#122088Purposesuicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cellsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
PE-Cas9
Plasmid#174851PurposeExpress PE-Cas9 in mammalian cellsDepositorInsertPE-Cas9
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTK095
Plasmid#59560PurposeSIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4 8xMS2DepositorInsertSGP(14) Kozak L7Ae-P2A-EYFP 4xFF4 8xMS2
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 CYP2A13
Plasmid#67103PurposeStandard for cell-free expression benchmarking.DepositorInsertCYP2A13 (CYP2A13 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONRPEX18Tp-SceI-pheS
Plasmid#111608PurposeGateway compatible allelic exchange vector for Burkholderia sp.DepositorTypeEmpty backboneUseAllelic exchange in burkholderia sp.TagsExpressionMutationPromoterAvailable sinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSBY3_ligD
Plasmid#104624PurposeCRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligDDepositorInsertFnCpf1
UseCRISPRTagsExpressionBacterialMutationFnCpf1 codon-optimized C.glutamicumPromoterhsp60Available sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTK296
Plasmid#59565PurposeSIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak EGFP-PESTDepositorInsertSGP(14) 2xK-turn Kozak EGFP-PEST
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFBNb-cALFA
Plasmid#159990PurposeFor the protein expression with ALFA tag at C-terminus in insect cellsDepositorTypeEmpty backboneUseTagsHRV3C protease cleavage site-ALFA tag-8histidineExpressionInsectMutationPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJBL723
Plasmid#140393PurposeExpresses HucR protein under the control of T7-lacO promoterDepositorInsertT7-lacO-HucR-T
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330A_dCas9-1x2
Plasmid#63596PurposeExpresses dCas9 and gRNADepositorInserthumanized S. pyogenes dCas9
UseCRISPRTags3xFLAGExpressionMammalianMutationD1361YPromoterCBhAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTol2-hspDRv7_scGstlt
Plasmid#108870PurposescGESTALT V7 lineage barcode for zebrafishDepositorInsertscGESTALT lineage tracing target V7
UseTol2 integration into zebrafish genomeTagsExpressionMutationPromoterAvailable sinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS-TarHom-Tol2-kanaR-cryaa-dsRed
Plasmid#74152PurposeBAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker dsRedDepositorInsertTol2-kanaR-cryaa-dsRed
UseTagsExpressionMutationPromoterAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
Gata4-GFP donor
Plasmid#113115PurposeDonor plasmid for knock-in eGFP fusing to the c-terminal of mouse Gata4 coding sequenceDepositorInsertGata4 donor region including homology arms and eGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMK377 (Hygro-P2A-mCherry2)
Plasmid#121188PurposeN-terminal taggingDepositorInsertHygro-P2A-mCherry2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
LV anti-FITC-LaG16-Y186F-T2A-BFP
Plasmid#194931PurposeLV construct encoding Y186F mutant sNRR-based SynNotch receptorDepositorInsertantiFITC-LaG16-sNRR(Y186F)-SynNotch-Gal4-VP64-T2A-BFP
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYL322d1/eGFP-C
Plasmid#183152PurposePlant transient expression vector containing eGFP tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of eGFP.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
IBMc090
Plasmid#161937PurposeCarries engineered transposon for IBMDepositorInsertpTHSSd_1V-WTR1R2-BbsI(G)-kanR-lacZalpha-SapI-WTR1R2
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGNW6
Plasmid#122093Purposesuicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cellsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFREE2-SpCas9-sgRNA
Plasmid#179582PurposeExpresses spCas9 and gRNA that targets ColE1 plasmids for curingDepositorInsertCas9
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-RNF169
Plasmid#119004PurposeMammalian expression of human RNF169 and BFPDepositorInsertRNF169 (RNF169 Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG1171 FIREmate-ECFP-Giantin
Plasmid#205166PurposeExpresses FIREmate-ECFP-Giantin in mammalian cellsDepositorInsertFIREmate-ECFP-Giantin
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETNb-cALFA
Plasmid#159989PurposeFor the protein expression with ALFA tag at C-terminus in E. coliDepositorTypeEmpty backboneUseTagsHRV3C protease cleavage site-ALFA tag-8histidineExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pS24·Pm-GFP
Plasmid#122594PurposeExpresses GFP under the regulatory control of Pm. Expression needs additional xyls geneDepositorInsertPm promoter
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPmAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJ211_WT_low
Plasmid#128848PurposeLow copy number plasmid for expression of E. coli flagellin from its natural promoterDepositorInsertFlagellin
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
LV anti-FITC-LaG16-Y186A-T2A-BFP
Plasmid#194932PurposeLV construct encoding Y186A mutant sNRR-based SynNotch receptorDepositorInsertantiFITC-LaG16-sNRR(Y186A)-SynNotch-Gal4-VP64-T2A-BFP
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA1-boxB-MS2-PP7
Plasmid#75397PurposesgRNA1-boxB-MS2-PP7DepositorInsertsgRNA1-boxB-MS2-PP7
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterU6Available sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
Rc/CMV cyclin D3
Plasmid#10912DepositorInsertcyclin D3 (CCND3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
nCas9-NG_P2A-mCherry
Plasmid#219934PurposeMammalian expression of Sp-nCas9-NG with P2A-mCherry marker for use with MOBE systemsDepositorInsertSp-nCas9(D10A)-NG_P2A-mCherry
UseCRISPRTagsSV40 bpNLS and SV40 bpNLS-P2A-mCherryExpressionMammalianMutationnCas9(D10A)PromoterCMVAvailable sinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB6574
Plasmid#106132PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMK408 (mAID-Nluc Hygro)
Plasmid#121198PurposeC-terminal taggingDepositorInsertmAID-Nluc Hygro
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3A9cbetaTCR
Plasmid#69600PurposeTCR beta chain transgene expression plasmid which has a unique ClaI site for the insertion of a TCR beta chain VDJ exonDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
mSA-phi29
Plasmid#210815Purposein vitro transcription of mRNADepositorInsertmSA-Phi29
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-DEST-hSOX2-EGFP-PuroTK
Plasmid#141026PurposeReporter integrationDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-Olig2
Plasmid#45844DepositorInsertOlig2 (Olig2 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromotertet-ONAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFastBac StrepII msfGFP TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-Rgfp)
Plasmid#55221DepositorTypeEmpty backboneUseTagsStrepII and msfGFPExpressionInsectMutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTK297
Plasmid#59566PurposeSIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak mKate-G8-L7Ae 4xFF4DepositorInsertSGP(14) Kozak mKate-G8-L7Ae 4xFF4
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTW211b5
Plasmid#136927PurposeDirected evolution of SpCas9DepositorInsertCas9(N)-NpuN
UseCRISPRTagsExpressionBacterialMutationsee manuscriptPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only