We narrowed to 11,928 results for: pcDNA

1. pcDNA3.1-ERBB4 (R544W)

   Plasmid

   #29531

   Depositor

   Yardena Samuels

   Article

   Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

   Insert

   ERBB4 (ERBB4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed arginine 544 to tryptophan

   Promoter

   Available since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.1/3xmyc_NLRP11-NACHT

   Plasmid

   #131199

   Purpose

   mammalian expression

   Depositor

   Thomas Kufer

   Article

   Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

   Insert

   NACHT of NLRP11 (NLRP11 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   contains only the NACHT domain (amino acids 125-5…

   Promoter

   pCMV

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ORF24-2xStrep pCDNA4

   Plasmid

   #129742

   Purpose

   Expresses KSHV ORF24 with Cterm 2xStrep tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

   Insert

   ORF24 (ORF24 Kaposi's sarcoma-associated herpesvirus (HHV-8))
   

   Use

   Tags

   2xStrep Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA4/TO-ORF66-2xStrep

   Plasmid

   #130953

   Purpose

   Expresses KSHV ORF66 a.a. 1-429 (full length) with a C-terminal 2xStrep tag in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

   Insert

   ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
   

   Use

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   ORF66 a.a. 1-429 (full-length)

   Promoter

   CMV

   Available since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA5/FRT/TO V5 DNAJB5

   Plasmid

   #19527

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DnaJ (Hsp40) homolog, subfamily B, member 5 (DNAJB5 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-Gα13(EE)-YFP

   Plasmid

   #171374

   Purpose

   Plasmid encoding Gα13 internally-tagged with the Citrine variant of YFP in the b/c loop

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   Ga13(EE)-YFP
   

   Use

   Tags

   Glu-Glu

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. GET1_pcDNA6.2/EmGFP-Bsd

   Plasmid

   #176985

   Purpose

   Mammalian expression vector encoding GET1 and EmGFP-Bsd

   Depositor

   Roger Reeves

   Article

   Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

   Insert

   GET1 (GET1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1(+)MBII-85-5'/3'ss mut

   Plasmid

   #67645

   Purpose

   MBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 5' and 3' splice sites were mutated to consensus sequences.

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

   Insert

   MBII85 snoRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   5' and 3' splice sites mutations

   Promoter

   Available since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCDNA3_PKA-Cα(K8,9A)-mEGFP

   Plasmid

   #168490

   Purpose

   Mammalian expression of the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP.

   Depositor

   Haining Zhong

   Article

   Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

   Insert

   the K8,9A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDNA3 ATP5O(5’UTR)-SL-FF

    Plasmid

    #85491

    Purpose

    Firefly luciferase under the control of ATP5O 5'UTR followed by a stem-loop

    Depositor

    Ivan Topisirovic

    Article

    Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

    Insert

    5'UTR of ATP5O followed by stem loop (ATP5PO Human)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-HA-DNM2-W525L

    Plasmid

    #155263

    Purpose

    Mammalian expression of rat DNM2 fused to HA

    Depositor

    David Rubinsztein

    Article

    Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

    Insert

    dynamin 2 (Dnm2 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Tryptophan 525 to Leucine

    Promoter

    Available since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA5-FRT-TO-FH-Nsp15

    Plasmid

    #157721

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp15 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. Myc-gamma3 (in pCDNA3.1)

    Plasmid

    #118041

    Purpose

    GABAA receptor expression (gamma3 subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG3 (Gabrg3 Rat)
    

    Use

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Myc-gamma1 (in pCDNA3.1)

    Plasmid

    #118038

    Purpose

    GABAA receptor expression (gamma1 subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG1 (Gabrg1 Rat)
    

    Use

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA FLAG mTOR ST

    Plasmid

    #22999

    Depositor

    Jing Huang

    Article

    Lomenick et al Proc Natl Acad Sci U S A. 2009 Dec 22;10

    Insert

    mTOR (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S2035T

    Promoter

    Available since

    May 18, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pCDNA3 ATP5O(TISU)-FF

    Plasmid

    #85489

    Purpose

    Firefly luciferase under the control of ATP5O TISU

    Depositor

    Ivan Topisirovic

    Article

    Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

    Insert

    Full TISU element of ATP5O (ATP5PO Human)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    2nd and 3rd codon of luciferase were modified fro…

    Promoter

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA4/HisMaxB-YAP2

    Plasmid

    #18997

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3-SLAP-FLAG

    Plasmid

    #74500

    Purpose

    Overexpression of SLAP in mammalian cells

    Depositor

    Lars Rönnstrand

    Article

    Kazi et al PLoS One. 2012;7(12):e53509. doi: 10.137

    Insert

    SLAP (SLA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N18K, G74A, and T149A

    Promoter

    CMV

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA4/TO-ORF24-3xFLAG

    Plasmid

    #138423

    Purpose

    Expresses full-length ORF24 with a C-terminal 3xFLAG tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    ORF24 (ORF24 KSHV (HHV-8))
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA-GATA2-DNzf

    Plasmid

    #1291

    Depositor

    Gokhan Hotamisligil

    Article

    Tong et al Science 2000 Oct 6;290(5489):134-8.

    Insert

    GATA2 (GATA2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S263G and M434V

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

21. pCDNA HA FAK Y577F

    Plasmid

    #50507

    Depositor

    Kenneth Yamada

    Article

    Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

    Insert

    protein tyrosine kinase, mutated
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Y577F

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

22. pCDNA3-HA/Bmal L95E

    Plasmid

    #47344

    Purpose

    PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Ha

    Expression

    Mammalian

    Mutation

    L95E

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

23. pcDNA-mTRAF3ip3-CFP

    Plasmid

    #125181

    Purpose

    Expresses mouse TRAF3IP3 in mammalian cells

    Depositor

    Shao-Cong Sun

    Insert

    TRAF3ip3 (Traf3ip3 Mouse)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    K87R, M283I, S359P, A506G- please see depositor c…

    Promoter

    CMV

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1-Nluc(W12stop)

    Plasmid

    #180502

    Purpose

    Mammalian expression of Nluc(W12stop)

    Depositor

    Masaharu Somiya

    Article

    Somiya et al bioRxiv 2022.01.25.477620

    Insert

    Nluc(W12stop)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    W12(UGG) to amber stop codon(UAG)

    Promoter

    CMV

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA-Flag-Trib1

    Plasmid

    #131156

    Purpose

    Mammalian expression of flag-tagged mouse TRIB1

    Depositor

    Warren Pear, Karen Keeshan

    Article

    Dedhia et al Blood. 2010 Aug 26;116(8):1321-8. doi: 1

    Insert

    Trib1 (Trib1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCDNA CrkII Y221F

    Plasmid

    #50732

    Purpose

    mammalian expression of CrkII Y221F mutant

    Depositor

    Kenneth Yamada

    Article

    Takino et al J Cell Sci. 2003 Aug 1;116(Pt 15):3145-5

    Insert

    CrkII Y221F (CRK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y221F

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

27. pcDNA3-CARD14-D176H

    Plasmid

    #69919

    Purpose

    Expresses mutant FLAG-CARD14 in mammalian cells

    Depositor

    Francesca Capon

    Article

    Berki et al J Invest Dermatol. 2015 Jul 23. doi: 10.

    Insert

    CARD14 isoform 3 (CARD14 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    D176H

    Promoter

    CMV

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

28. pcDNA6-chCAR-MycHis

    Plasmid

    #87232

    Purpose

    Mammalian expression of chicken CAR

    Depositor

    Fritz Rathjen

    Insert

    CXADR (CXADR Chicken)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3-DKAR-Nuc

    Plasmid

    #45499

    Depositor

    Alexandra Newton

    Article

    Bossuyt et al J Biol Chem. 2011 Sep 23;286(38):33390-4

    Insert

    DKAR-Nuc
    

    Use

    Tags

    CFP, NLS, and YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

30. pcDNA5FRTTO-NHASt-codoptUL50

    Plasmid

    #135101

    Purpose

    Codon-optimized HSV-1 UL50 with N-terminal HAStrep tag

    Depositor

    Markus Landthaler

    Article

    Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

    Insert

    HSV-1 UL50 codon optimized
    

    Use

    Tags

    HA-Strep-Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3 HRPT2 136X

    Plasmid

    #11049

    Depositor

    Matthew Meyerson

    Article

    Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

    Insert

    HRPT2 136X (CDC73 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    truncation mutant with a stop codon introduced at…

    Promoter

    Available since

    Dec. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

32. pcDNA3-gap16-ClopHensor

    Plasmid

    #26006

    Depositor

    Daniele Arosio

    Article

    Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

    Insert

    gap16-ClopHensor
    

    Use

    Tags

    Gap16 and Strep-II

    Expression

    Mammalian

    Mutation

    N-term (16AA) of Gap-43

    Promoter

    Available since

    Sept. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA-Flag-EcoCas6

    Plasmid

    #106274

    Purpose

    Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    EcoCasE
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA-p160(1-200)

    Plasmid

    #43

    Depositor

    Bruce Spiegelman

    Article

    Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

    Insert

    p160 myb binding protein (Mybbp1a Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

35. pcDNA5-FRT-TO-FH-Nsp7

    Plasmid

    #157689

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp7 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pcDNA3.1(+)-FluoSTEP-EKAR

    Plasmid

    #181858

    Purpose

    Genetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    FluoSTEP-EKAR
    

    Use

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCDNA3.1-nAChr-beta3

    Plasmid

    #48811

    Purpose

    This report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.

    Depositor

    Jerry Stitzel, Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr Beta 3 subunit, isoform 1 (Chrnb3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    *

    Promoter

    CMV

    Available since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA5/FRT/TO DNAJB2b

    Plasmid

    #19470

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB2b (DNAJB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. a4 ecto pcDNA3.1 hygro(-)

    Plasmid

    #113406

    Purpose

    expression of human alpha 4 ectodomain with purification tags and C-terminal clasp

    Depositor

    Timothy Springer

    Article

    Li et al J Cell Biol. 2018 Jan 2;217(1):397-412.

    Insert

    human alpha 4 ectodomain
    

    Use

    Tags

    Strep II tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3 GATA1 K137R

    Plasmid

    #85694

    Purpose

    Mammalian expression of mouse GATA1 with K137R mutation

    Depositor

    Giannino Del Sal, Licio Collavin

    Article

    Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

    Insert

    GATA1 (Gata1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K137R (SUMO-1 attachment site)

    Promoter

    CMV

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only